SPG11 (NM_001160227) Human Untagged Clone

CAT#: SC327719

SPG11 (untagged)-Human spastic paraplegia 11 (autosomal recessive) (SPG11) transcript variant 2



  "NM_001160227" in other vectors (2)

CNY 26,790.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal SPG11 Antibody
    • 100 ug

CNY 5,808.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ALS5; CMT2X; KIAA1840
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001160227, the custom clone sequence may differ by one or more nucleotides
ATGGCTGCAGAGGAAGGGGTCGCGAGTGCTGCTTCCGCCGGCGGTAGCTGGGGCACCGCG
GCCATGGGGCGGGTTCTACCGATGCTGTTGGTGCCAGTCCCCGCCGAGGCGATGGGGCAG
CTCGGCTCCCGGGCGCAGCTGCGCACACAGCCGGAGGCTCTGGGGAGCCTGACGGCTGCG
GGCAGCCTCCAAGTGCTTTCTTTGACGCCTGGCAGCCGGGGCGGGGGTCGCTGCTGCCTG
GAGGGCCCCTTCTGGCACTTTCTATGGGAGGATTCTCGTAACAGCAGCACACCAACTGAA
AAGCCCAAACTGCTCGCTCTTGGTGAAAATTATGAACTGCTTATCTATGAATTTAATTTG
AAAGATGGAAGATGTGATGCAACCATTTTGTATAGCTGTAGTAGGGAGGCATTGCAAAAG
CTCATTGACGATCAAGATATCAGTATTTCCTTATTGTCTTTGAGAATCCTGTCATTTCAC
AATAACACATCATTACTGTTCATCAACAAATGTGTCATCCTACATATTATATTTCCTGAA
AGAGATGCTGCAATTAGAGTACTCAACTGTTTCACACTTCCCTTGCCTGCACAGGCAGTG
GACATGATTATTGACACGCAGCTCTGCAGAGGAATTCTTTTTGTTTTGAGTAGTTTAGGC
TGGATCTACATTTTTGATGTTGTGGATGGTACATATGTAGCTCATGTGGATTTAGCACTT
CACAAAGAAGACATGTGTAATGAGCAGCAACAGGAGCCAGCCAAGATTTCTTCATTTACT
TCACTGAAAGTTTCTCAAGACCTCGATGTTGCAGTGATTGTCAGCTCCTCCAACTCCGCA
GTTGCTCTTAACTTAAATTTGTATTTCAGGCAACACCCAGGACACCTACTGTGTGAAAGA
ATACTAGAAGATCTTCCTATTCAAGGACCTAAGGGCGTAGATGAAGATGATCCTGTTAAC
TCTGCCTACAACATGAAACTGGCCAAGTTTTCCTTCCAAATTGATAGGTCTTGGAAAGCC
CAGCTATCATCATTGAATGAAACAATAAAGAACTCCAAACTGGAGGTTTCCTGTTGTGCT
CCATGGTTCCAGGATATTTTGCATTTGGAGTCACCTGAATCTGGTAACCACAGTACAAGT
GTGCAGAGCTGGGCCTTCATTCCACAGGACATAATGCATGGGCAATATAATGTTCTACAG
AAAGATCATGCCAAGACCAGTGATCCAGGAAGATCATGGAAAATAATGCACATCAGTGAA
CAAGAGGAACCCATAGAGCTTAAATGTGTGTCTGTGACAGGATTCACTGCACTGTTTACT
TGGGAAGTGGAAAGGATGGGCTATACCATTACCCTCTGGGATTTGGAGACCCAGGGCATG
CAGTGTTTTTCCCTTGGCACAAAGTGTATTCCTGTAGACAGTAGTGGAGACCAGCAGCTG
TGCTTTGTTTTGACAGAGAATGGACTCTCTCTGATTTTGTTTGGTTTGACTCAAGAAGAG
TTTTTAAACAGACTCATGATCCATGGAAGTGCCAGCACTGTGGACACTCTTTGTCATCTC
AATGGCTGGGGAAGGTGCTCAATTCCCATACATGCACTAGAGGCCGGGATAGAAAATCGT
CAGCTGGACACAGTAAATTTCTTTTTGAAGAGCAAGGAAAATCTTTTTAATCCATCCTCA
AAATCTTCTGTATCTGATCAGTTTGATCACTTGTCATCCCATTTATATTTAAGAAATGTG
GAAGAGCTGATACCAGCATTGGATTTACTTTGCTCGGCAATTAGAGAAAGTTATTCTGAA
CCCCAAAGCAAACACTTTTCAGAACAATTGCTTAATCTTACACTGTCTTTCCTTAACAAC
CAAATAAAGGAGCTTTTCATTCACACTGAAGAACTAGATGAACATCTGCAAAAAGGAGTG
AACATTTTGACTAGCTACATTAATGAACTTCGAACCTTCATGATAAAGTTTCCTTGGAAG
CTAACAGATGCTATAGATGAATATGATGTACATGAAAATGTCCCCAAAGTAAAGGAGAGC
AATATATGGAAGAAACTCAGCTTTGAGGAAGTTATTGCCAGCGCCATTTTAAACAACAAA
ATACCAGAGGCACAGACTTTCTTCAGGATTGATAGTCATTCTGCTCAAAAACTTGAGGAG
CTTATTGGCATAGGCCTAAATTTGGTCTTTGACAATTTAAAAAAGAACAATATAAAGGAA
GCCTCTGAACTTTTGAAGAATATGGGGTTTGATGTAAAAGGCCAATTGCTCAAGATCTGC
TTCTATACAACTAATAAAAATATACGTGACTTTTTGGTTGAAATTTTAAAAGAAAAAAAT
TATTTTTCTGAAAAAGAGAAAAGAACTATAGACTTCGTGCATCAAGTTGAGAAGCTTTAT
TTGGGACATTTCCAAGAAAATATGCAAATCCAGTCATTTCCCAGGTACTGGATAAAGGAA
CAAGATTTTTTCAAGCACAAGTCTGTTTTGGACTCATTCCTGAAATATGATTGTAAAGAT
GAATTTAACAAACAGGACCATAGAATTGTGTTAAATTGGGCTCTGTGGTGGGATCAACTA
ACACAAGAATCCATCCTTCTCCCCAGGATAAGTCCAGAAGAATACAAATCATATTCCCCT
GAAGCCCTCTGGAGATACCTCACAGCTCGCCATGATTGGTTAAACATTATCTTATGGATT
GGAGAATTTCAAACCCAGCATAGTTATGCTTCACTTCAGCAGAACAAATGGCCCCTTCTG
ACTGTTGATGTTATTAACCAGAATACTTCCTGTAACAACTACATGAGGAATGAAATTTTA
GATAAGCTGGCCAGGAATGGGGTTTTTTTGGCATCTGAACTGGAAGACTTTGAATGCTTC
CTCCTAAGACTGAGCCGTATTGGAGGTGTAATACAGGATACCCTCCCTGTTCAAAACTAC
AAGACCAAAGAAGGTTGGGATTTCCATTCTCAATTCATTCTCTATTGTTTGGAGCACAGT
CTGCAGCATCTTCTTTATGTCTACCTTGACTGTTACAAACTTAGTCCTGAAAATTGTCCC
TTTTTGGAAAAAAAAGAGTTACATGAAGCACACCCTTGGTTTGAATTTTTAGTTCAGTGT
CGACAAGTTGCCAGTAACTTAACAGATCCCAAACTGATCTTCCAGGCTAGCCTTGCAAAT
GCTCAGATTTTGATTCCCACCAATCAGGCCAGTGTAAGCAGTATGCTATTGGAAGGACAT
ACCCTCCTGGCCCTTGCTACTACAATGTATTCTCCTGGGGGTGTCAGTCAGGTTGTTCAG
AATGAAGAAAATGAAAACTGTTTGAAGAAAGTGGATCCCCAGCTATTGAAGATGGCATTA
ACTCCTTACCCCAAGCTAAAAACTGCTCTCTTCCCACAGTGCACTCCTCCTAGTGTCCTG
CCATCTGATATTACAATCTACCACCTTATTCAGTCATTATCACCCTTTGATCCTAGCAGA
TTGTTTGGCTGGCAGTCTGCTAACACACTAGCTATAGGAGATGCATGGAGTCATCTCCCA
CATTTCTCTAGCCCTGACCTGGTTAATAAATATGCTATAGTGGAACGTCTGAATTTTGCT
TATTATTTACATAATGGGCGGCCATCATTTGCATTTGGTACTTTTCTGGTCCAGGAATTA
ATCAAGAGCAAGACTCCCAAGCAGCTGATCCAGCAAGTAGGCAATGAAGCCTATGTTATA
GGGCTCTCCTCCTTCCACATACCTTCAATAGGAGCTGCATGTGTTTGTTTCTTAGAATTG
CTTGGCCTTGACAGCCTCAAGCTCAGAGTTGATATGAAAGTGGCCAATATAATTTTGAGC
TACAAGTGCAGAAATGAAGATGCTCAGTACAGCTTTATCAGAGAGTCTGTAGCCGAAAAA
CTATCTAAACTAGCTGATGGTGAAAAGACAACCACAGAAGAATTGCTTGTTCTCTTAGAA
GAAGGTACATGGAACAGCATTCAGCAACAGGAAATAAAGAGGTTATCCAGTGAATCTAGC
AGCCAATGGGCATTAGTGGTGCAGTTCTGCAGGCTACACAATATGAAACTAAGCATATCT
TACCTTAGAGAATGTGCCAAAGCAAATGATTGGCTGCAGTTCATTATTCACAGCCAACTC
CACAACTACCACCCAGCAGAGGTGAAATCCCTTATCCAGTACTTCAGCCCAGTCATTCAA
GACCACTTAAGGCTGGCTTTTGAGAACTTGCCCTCAGTGCCCACCTCCAAAATGGACAGC
GATCAAGTCTGCAATAAGTGCCCCCAGGAACTTCAAGGAAGCAAACAAGAGATGACCGAT
TTATTTGAAATTCTGCTCCAATGCTCAGAGGAGCCAGACTCCTGGCACTGGCTTCTGGTT
GAAGCAGTGAAACAACAGGCCCCTATCCTCAGTGTTCTGGCCTCATGTCTCCAGGGTGCC
AGTGCCATTTCTTGTCTCTGTGTTTGGATCATCACTTCTGTGGAGGACAATGTTGCAACT
GAAGCAATGGGACACATTCAGGACTCAACAGAGGACCATACCTGGAACCTTGAGGATCTT
TCAGTCATCTGGAGAACATTATTAACAAGACAAAAGAGCAAAACTCTCATCAGAGGTTTC
CAGCTTTTCTTTAAGGATTCCCCGTTACTACTGGTGATGGAGATGTATGAACTGTGTATG
TTCTTCAGGAATTATAAAGAAGCTGAAGCTAAACTTCTGGAGTTTCAGAAGAGCCTTGAA
ACGCTTAACACAGCAGCCACAAAGGTCCACCCTGTCATCCCTGCCATGTGGCTGGAGGAT
CAGGTGTGTTTCCTTTTGAAGCTTATGCTACAGCAGTGTAAGACCCAGTATGAGCTGGGG
AAGCTTTTACAGCTCTTTGTTGAAAGAGAGCATCTCTTCTCTGATGGTCCAGATGTGAAA
AAGCTTTGCATCCTTTGCCAGATTTTGAAGGATACATCCATAGCCATTAATCATACAATT
ATTACCAGCTACAGCATTGAGAATCTTCAGCATGAATGTAGATCTATTTTGGAAAGACTG
CAGACAGATGGACAATTCGCTTTGGCCAGGAGGGTAGCAGAATTAGCTGAGTTACCTGTG
GACAACTTGGTTATTAAAGAGATAACACAGGAAATGCAGACCCTAAAACACATTGAACAG
TGGTCACTAAAACAAGCAAGAATTGACTTCTGGAAAAAATGCCATGAGAATTTTAAGAAA
AATTCAATTTCAAGCAAAGCAGCTTCTTCCTTTTTCTCAACCCAGGCCCATGTGGCATGT
GAGCACCCAACTGGATGGAGCAGCATGGAGGAGCGCCATCTGCTGCTCACCTTGGCAGGG
CACTGGCTTGCCCAGGAGGACGTGGTGCCCTTGGATAAGCTGGAGGAGCTGGAGAAGCAG
ATCTGGCTGTGCCGCATCACCCAGCACACTCTTGGAAGAAATCAGGAGGAAACAGAGCCC
AGATTTTCTCGACAGATCTCAACTAGTGGTGAACTTTCCTTTGATAGTTTAGCCAGTGAG
TTTTCCTTCTCCAAGTTGGCTGCTCTGAACACATCAAAATACTTAGAACTTAACAGCCTT
CCATCCAAAGAGACATGCGAGAATAGATTGGATTGGAAAGAGCAGGAGTCACTAAACTTT
TTGATTGGGCGCCTACTGGATGATGGCTGTGTGCATGAAGCAAGTAGAGTATGCCGGTAT
TTTCATTTTTATAATCCAGATGTCGCCTTGGTATTGCACTGCAGAGCACTGGCCTCAGGG
GAAGCTAGTATGGAGGATCTGCACCCAGAGATCCATGCTCTCCTACAAAGTGCTGAGCTG
CTTGAGGAAGAAGCACCCGACATTCCCCTAAGGAGAGTCCACAGCAGACATAAGCAGATG
TTCAACCCAACAGAGGAAAGCCAGACATTTCTTCAGCTGACCACTCTGTGTCAAGACCGC
ACATTGGTAGGCATGAAGTTGTTGGATAAGATTTCCTCCGTTCCCCATGGGGAACTGTCT
TGCACCACAGAGCTCCTGATCCTGGCCCATCATTGCTTCACCCTGACGTGCCACATGGAG
GGCATCATCCGAGTCCTACAGGCCGCCCACATGCTCACAGATAACCACCTGGCCCCCAGT
GAGGAGTATGGGCTGGTGGTACGGCTCCTCACTGGCATTGGAAGGTACAACGAGATGACA
TACATATTTGATTTGCTGCATAAAAAGCACTACTTTGAAGTGCTAATGAGGAAGAAGTTG
GATCCGAGTGGTACCCTGAAAACAGCCCTGCTGGACTACATCAAACGCTGCCGTCCTGGA
GACAGTGAAAAGCACAATATGATTGCCCTGTGCTTCAGCATGTGCCGGGAGATTGGCGAG
AACCACGAGGCAGCTGCCCGCATCCAACTGAAATTGATTGAGTCTCAGCCCTGGGAGGAC
AGCCTCAAGGATGGGCACCAGCTGAAACAACTGCTGCTGAAGGCCCTGACTCTGATGTTG
GATGCAGCAGAGAGTTATGCCAAGGACTCCTGTGTGCGACAGGCCCAGCACTGTCAGCGG
CTCACCAAGTTGATAACTCTGCAGATTCACTTTCTGAACACTGGCCAGAACACAATGCTC
ATCAACTTGGGCCGCCACAAGCTGATGGACTGTATTCTGGCCCTACCTCGGTTCTACCAG
GCTTCTATTGTGGCTGAGGCCTACGATTTTGTTCCAGATTGGGCTGAAATTTTATACCAG
CAAGTGATTCTTAAAGGAGACTTTAATTACTTGGAAGAATTTAAGCAGCAAAGGTTATTA
AAGTCCAGTATATTTGAAGAGATTTCCAAAAAATATAAACAACATCAGCCTACTGACATG
GTCATGGAAAACCTGAAGAAATTACTCACATATTGTGAAGATGTTTACCTGTATTACAAG
TTGGCATACGAACACAAGTTTTATGAAATTGTAAATGTGCTTCTGAAGGACCCTCAGACA
GGTTGCTGTCTAAAGGACATGCTAGCAGGT
Restriction Sites Please inquire     
ACCN NM_001160227
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001160227.1, NP_001153699.1
RefSeq Size 7478 bp
RefSeq ORF 6993 bp
Locus ID 80208
UniProt ID Q96JI7
Gene Summary The protein encoded by this gene is a potential transmembrane protein that is phosphorylated upon DNA damage. Defects in this gene are a cause of spastic paraplegia type 11 (SPG11). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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