RASGRP3 (NM_015376) Human Untagged Clone
CAT#: SC327632
RASGRP3 (untagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3) transcript variant 3
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GRP3 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_015376, the custom clone sequence may differ by one or more nucleotides
ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATT GAGATGTTTGATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTG ATGCACCGATGGTATTTATCTTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGA AATGCCACTGGAGAAAGCTGCAATGAATTTCGATTAAAGATCTGCTACTTCATGAGGTAC TGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGATCTTGGTTTGATTCGTATGACTGAG GAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTCAGCCTCATCGACATA TCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTATCCAAG AAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTC ACTTTTCTGGAGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTC ATCCATGGCTGCCTGGAGAATAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGA ATCTCTAAGTGGGTCCAGTTGATGGTTCTTAGCAAACCAACCCCCCAGCAAAGGGCAGAA GTCATCACAAAGTTTATCAATGTTGCAAAGAAGCTCCTTCAGCTCAAAAATTTTAACACC CTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATTTCACGCCTCAAAGAGACCCAT TCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAGTTGGTCTCCTCC AACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATCCCC ATCCTTGGAGTACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACA GAGGAGAACAAAGTGAACATTGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTA GTCTCCCTGCAGAATGCCTCTCACCACTTAGAACCCAACATGGATTTGATCAACCTGCTC ACGCTTTCCCTGGACCTCTATCACACTGAAGATGATATTTACAAACTGTCACTGGTGCTG GAGCCTAGAAATTCTAAATCGCCTACCTCCCCTACGACGCCCAACAAGCCTGTGGTACCC CTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCCACGGTCATCAACAAGCACATA AGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGATGGGTACATTTCC CAAGAGGACTTTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTTCTG GACAAAGATCAGGATGGCCTAATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCT AAATCCCAACTACACTGTAAAATGGGACCAGGATTTATCCATAATTTTCAGGAGATGACC TATCTCAAGCCAACCTTCTGCGAACACTGTGCGGGATTTCTCTGGGGCATAATCAAGCAA GGATACAAATGCAAAGACTGTGGAGCCAATTGTCACAAACAGTGCAAAGACCTCCTGGTT CTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCCTTGAGCAGTGGTCATGGGTCACTGCCT GGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAGTTCCCTGGAGTCACTGCT GGACACAGGGATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCTCGCAAGATC TCTGTGAGGCTACAGAGGGCCACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCA GAGGCTGGCTGGGGGGACTCGGGGTCCCACACCTTCCCTAAAATGAAATCCAAGTTCCAT GACAAAGCAGCAAAGGACAAAGGCTTTGCCAAATGGGAAAATGAGAAGCCCAGGGTGCAT GCTGGTGTGGATGTTGTAGACCGGGGCACGGAGTTTGAACTTGACCAGGATGAAGGAGAA GAGACCAGACAGGATGGTGAGGATGGC |
Restriction Sites | Please inquire |
ACCN | NM_015376 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_015376.2, NP_056191.1 |
RefSeq Size | 4353 bp |
RefSeq ORF | 2070 bp |
Locus ID | 25780 |
UniProt ID | Q8IV61 |
Domains | RasGEFN, RasGEF, EFh, DAG_PE-bind |
Protein Pathways | B cell receptor signaling pathway, MAPK signaling pathway |
Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2) which is 1 aa shorter than isoform 1. Variants 3 and 7-10 all encode the same isoform (2). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228997 | RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 3,990.00 |
|
RC228997L3 | Lenti-ORF clone of RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 5,890.00 |
|
RC228997L4 | Lenti-ORF clone of RASGRP3 (mGFP-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 5,890.00 |
|
RG228997 | RASGRP3 (tGFP-tagged) - Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 3 |
CNY 4,370.00 |
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