RASGRP3 (NM_015376) Human Untagged Clone

CAT#: SC327632

RASGRP3 (untagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3) transcript variant 3



  "NM_015376" in other vectors (4)

CNY 7,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
RASGRP3 mouse monoclonal antibody, clone OTI2C11 (formerly 2C11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GRP3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_015376, the custom clone sequence may differ by one or more nucleotides
ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATT
GAGATGTTTGATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTG
ATGCACCGATGGTATTTATCTTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGA
AATGCCACTGGAGAAAGCTGCAATGAATTTCGATTAAAGATCTGCTACTTCATGAGGTAC
TGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGATCTTGGTTTGATTCGTATGACTGAG
GAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTCAGCCTCATCGACATA
TCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTATCCAAG
AAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTC
ACTTTTCTGGAGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTC
ATCCATGGCTGCCTGGAGAATAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGA
ATCTCTAAGTGGGTCCAGTTGATGGTTCTTAGCAAACCAACCCCCCAGCAAAGGGCAGAA
GTCATCACAAAGTTTATCAATGTTGCAAAGAAGCTCCTTCAGCTCAAAAATTTTAACACC
CTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATTTCACGCCTCAAAGAGACCCAT
TCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAGTTGGTCTCCTCC
AACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATCCCC
ATCCTTGGAGTACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACA
GAGGAGAACAAAGTGAACATTGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTA
GTCTCCCTGCAGAATGCCTCTCACCACTTAGAACCCAACATGGATTTGATCAACCTGCTC
ACGCTTTCCCTGGACCTCTATCACACTGAAGATGATATTTACAAACTGTCACTGGTGCTG
GAGCCTAGAAATTCTAAATCGCCTACCTCCCCTACGACGCCCAACAAGCCTGTGGTACCC
CTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCCACGGTCATCAACAAGCACATA
AGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGATGGGTACATTTCC
CAAGAGGACTTTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTTCTG
GACAAAGATCAGGATGGCCTAATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCT
AAATCCCAACTACACTGTAAAATGGGACCAGGATTTATCCATAATTTTCAGGAGATGACC
TATCTCAAGCCAACCTTCTGCGAACACTGTGCGGGATTTCTCTGGGGCATAATCAAGCAA
GGATACAAATGCAAAGACTGTGGAGCCAATTGTCACAAACAGTGCAAAGACCTCCTGGTT
CTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCCTTGAGCAGTGGTCATGGGTCACTGCCT
GGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAGTTCCCTGGAGTCACTGCT
GGACACAGGGATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCTCGCAAGATC
TCTGTGAGGCTACAGAGGGCCACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCA
GAGGCTGGCTGGGGGGACTCGGGGTCCCACACCTTCCCTAAAATGAAATCCAAGTTCCAT
GACAAAGCAGCAAAGGACAAAGGCTTTGCCAAATGGGAAAATGAGAAGCCCAGGGTGCAT
GCTGGTGTGGATGTTGTAGACCGGGGCACGGAGTTTGAACTTGACCAGGATGAAGGAGAA
GAGACCAGACAGGATGGTGAGGATGGC
Restriction Sites Please inquire     
ACCN NM_015376
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015376.2, NP_056191.1
RefSeq Size 4353 bp
RefSeq ORF 2070 bp
Locus ID 25780
UniProt ID Q8IV61
Domains RasGEFN, RasGEF, EFh, DAG_PE-bind
Protein Pathways B cell receptor signaling pathway, MAPK signaling pathway
Gene Summary The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017]
Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2) which is 1 aa shorter than isoform 1. Variants 3 and 7-10 all encode the same isoform (2).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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