CHFR (NM_001161346) Human Untagged Clone
CAT#: SC327619
CHFR (untagged)-Human checkpoint with forkhead and ring finger domains (CHFR) transcript variant 3
CNY 10,450.00
Cited in 1 publication. |
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RNF116; RNF196 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001161346, the custom clone sequence may differ by one or more nucleotides
ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTG CGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATC GGGCGGAGACGAGGTTGCGACCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCAC TGTAGAATTGTAGTGGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGT GGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAGACTGGG GATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGCATACCTCTAT GAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGATACCTCAGGTGCAGGT GCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTCGCCCGCCACTCAGGTGTGCTTT GAGGAACCACAGCCATCAACATCGACGTCAGACCTCTTCCCCACAGCCTCGGCCTCTTCC ACGGAGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGC ATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCTCA GCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAGGAT TTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTG TTGGTCGCACAACCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCT GGGAAGCCAGACAAGATGGAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCAC GACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCTGG ATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGGATCTGTAAAAAC CACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGACAAGAGTCGCAGT GAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCC AAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCA GACGTTGACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCT GAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGA GCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGTCAGCCTGACGACAGCAGTCCAG GATTACGTGTGCCCTCTGCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCCATG CCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTC TGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGC CTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAAC AACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAA AACATGTTGACCGAGAGCCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTAC AGAGTCACGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTG ACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCCGT CCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCACGCCATGAAA TTCAATCATATCTGTGAACAGACAAGGTTCAAAAAC |
Restriction Sites | Please inquire |
ACCN | NM_001161346 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001161346.1, NP_001154818.1 |
RefSeq Size | 3255 bp |
RefSeq ORF | 1959 bp |
Locus ID | 55743 |
UniProt ID | Q96EP1 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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High Beclin-1 and ARID1A expression corelates with poor survival and high recurrence in intrahepatic cholangiocarcinoma: a histopathological retrospective study
,Bi, C;Liu, M;Rong, W;Wu, F;Zhang, Y;Lin, S;Liu, Y;Wu, J;Wang, L;,
BMC Cancer
,PubMed ID 30849962
[CHFR]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228984 | CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3 |
CNY 4,560.00 |
|
RC228984L3 | Lenti-ORF clone of CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3 |
CNY 6,460.00 |
|
RC228984L4 | Lenti-ORF clone of CHFR (mGFP-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3 |
CNY 6,460.00 |
|
RG228984 | CHFR (tGFP-tagged) - Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3 |
CNY 5,040.00 |