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EME1 (NM_001166131) Human Untagged Clone
CAT＃: SC327602
EME1 (untagged)-Human essential meiotic endonuclease 1 homolog 1 (S. pombe) (EME1) transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MMS4L; SLX2A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327602 representing NM_001166131.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTCTAAAGAAGTCATCACCCTCACTGGATTCTGGTGATAGTGACTCTGAGGAGTTGCCAACATTT GCCTTTCTGAAGAAGGAACCATCTTCAACAAAGAGGAGACAGCCTGAAAGGGAAGAGAAGATTGTAGTG GTTGACATCTCAGATTGTGAAGCCTCCTGTCCTCCAGCACCAGAGTTATTTTCACCACCTGTCCCAGAA ATAGCTGAAACTGTCACACAAACACAGCCAGTCAGGTTGCTAAGCAGTGAAAGTGAAGATGAAGAAGAA TTTATTCCTCTGGCTCAAAGGCTTACATGTAAGTTTCTGACCCACAAGCAACTGAGCCCTGAGGACTCT AGCTCCCCAGTTAAAAGTGTTTTGGATCATCAAAATAATGAAGGTGCATCATGTGACTGGAAAAAGCCC TTTCCAAAGATCCCTGAAGTTCCCCTCCATGATACCCCAGAGAGGAGTGCAGCAGATAACAAGGACCTG ATCTTAGATCCATGCTGTCAGCTTCCAGCCTACCTGTCTACCTGCCCTGGCCAGAGCAGCAGCTTGGCA GTAACCAAAACAAATTCTGACATCCTTCCACCCCAGAAGAAAACCAAGCCGAGTCAGAAGGTCCAGGGA AGAGGCTCACACGGATGCCGGCAGCAGAGACAAGCAAGGCAGAAGGAAAGCACCCTGAGAAGACAGGAA AGAAAGAATGCAGCACTGGTTACCAGGATGAAAGCCCAGAGGCCAGAGGAATGCTTAAAACACATCATT GTAGTGCTGGATCCAGTGCTCTTACAGATGGAAGGTGGGGGCCAGCTCCTAGGAGCACTGCAGACCATG GAGTGCCGCTGTGTGATTGAGGCGCAGGCTGTGCCTTGCAGTGTCACTTGGAGGAGAAGGGCTGGGCCG TCTGAGGACAGAGAGGACTGGGTGGAGGAGCCAACAGTACTGGTGTTGCTCCGGGCAGAGGCATTTGTG TCCATGATCGACAATGGAAAGCAGGGAAGCCTGGACAGCACTATGAAAGGGAAGGAAACGCTTCAGGGC TTTGTAACTGACATCACAGCAAAGACAGCAGGGAAAGCTCTGTCACTGGTGATTGTGGATCAGGAGAAA TGCTTCAGCCTGGAGCTGCTGTTCTTTGATTTCCTCCCCTGCACCAGTGCTCAGAATCCTCCAAGAAGA GGGAAACAGGGAGCAAATAAACAGACCAAGAAGCAGCAGCAGAGACAACCAGAGGCCAGCATAGGGTCC ATGGTATCCAGGGTAGACGCTGAAGAGGCATTGGTGGATCTGCAGCTACACACAGAAGCCCAGGCTCAA ATTGTGCAGAGCTGGAAAGAGCTGGCCGACTTCACATGCGCATTCACAAAGGCTGTGGCTGAGGCGCCC TTCAAGAAGCTCCGAGATGAAACTACCTTCTCCTTCTGTCTGGAGAGTGACTGGGCTGGAGGGGTGAAG GTGGACCTTGCTGGCAGGGGACTCGCACTAGTCTGGAGGAGACAGATTCAGCAGCTGAACCGAGTCAGC CTGGAAATGGCCAGTGCAGTTGTGAATGCCTATCCCTCCCCACAGCTCCTGGTACAGGCTTATCAGCAG TGTTTTTCGGATAAAGAACGCCAGAATTTGCTCGCAGACATACAGGTGCGCCGTGGGGAAGGTGTGACA TCCACTTCTCGCCGCATTGGACCAGAACTATCCAGGCGTATCTACCTTCAGATGACCACTTTACAGCCA CATCTCTCTTTAGATAGTGCTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001166131 |
| Insert Size | 1752 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001166131.1 |
| RefSeq Size | 2371 bp |
| RefSeq ORF | 1752 bp |
| Locus ID | 146956 |
| UniProt ID | Q96AY2 |
| Protein Pathways | Homologous recombination |
| MW | 64.8 kDa |
| Gene Summary | This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228967 | EME1 (Myc-DDK-tagged)-Human essential meiotic endonuclease 1 homolog 1 (S. pombe) (EME1), transcript variant 1 |
CNY 4,344.00 |
|
| RC228967L3 | Lenti-ORF clone of EME1 (Myc-DDK-tagged)-Human essential meiotic endonuclease 1 homolog 1 (S. pombe) (EME1), transcript variant 1 |
CNY 5,890.00 |
|
| RC228967L4 | Lenti-ORF clone of EME1 (mGFP-tagged)-Human essential meiotic endonuclease 1 homolog 1 (S. pombe) (EME1), transcript variant 1 |
CNY 5,890.00 |
|
| RG228967 | EME1 (tGFP-tagged) - Human essential meiotic endonuclease 1 homolog 1 (S. pombe) (EME1), transcript variant 1 |
CNY 4,370.00 |
