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CYP51A1 (NM_001146152) Human Untagged Clone
CAT＃: SC327516
CYP51A1 (untagged)-Human cytochrome P450 family 51 subfamily A polypeptide 1 (CYP51A1) transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001146152, the custom clone sequence may differ by one or more nucleotides
ATGGTAGGCAAGACATTTACTTACCTTCTGGGGAGTGATGCTGCTGCACTGCTTTTTAAT AGTAAAAATGAAGACCTGAATGCAGAAGATGTCTACAGTCGCCTGACAACACCTGTGTTT GGGAAGGGAGTTGCATACGATGTGCCTAATCCAGTTTTCTTGGAGCAGAAGAAAATGTTA AAAAGTGGCCTTAACATAGCCCACTTTAAACAGCATGTTTCTATAATTGAAAAAGAAACA AAGGAATACTTTGAGAGTTGGGGAGAAAGTGGAGAAAAAAATGTGTTTGAAGCTCTTTCT GAGCTCATAATTTTAACAGCTAGCCATTGTTTGCATGGAAAGGAAATCAGAAGTCAACTC AATGAAAAGGTAGCACAGCTGTATGCAGATTTGGATGGAGGTTTCAGCCATGCAGCCTGG CTCTTACCAGGTTGGCTGCCTTTGCCTAGTTTCAGACGCAGGGACAGAGCTCATCGGGAA ATCAAGGATATTTTCTATAAGGCAATCCAGAAACGCAGACAGTCTCAAGAAAAAATTGAT GACATTCTCCAAACTTTACTAGATGCTACATACAAGGATGGGCGTCCTTTGACTGATGAT GAAGTAGCAGGGATGCTTATTGGATTACTCTTGGCAGGGCAGCATACATCCTCAACTACT AGTGCTTGGATGGGCTTCTTTTTGGCCAGAGACAAAACACTTCAAAAAAAATGTTATTTA GAACAGAAAACAGTCTGTGGAGAGAATCTGCCTCCTTTAACTTATGACCAGCTCAAGGAT CTAAATTTACTTGATCGCTGTATAAAAGAAACATTAAGACTTAGACCTCCTATAATGATC ATGATGAGAATGGCCAGAACTCCTCAGACTGTGGCAGGGTATACCATTCCTCCAGGACAT CAGGTGTGTGTTTCTCCCACTGTCAATCAAAGACTTAAAGACTCATGGGTAGAACGCCTG GACTTTAATCCTGATCGCTACTTACAGGATAACCCAGCATCAGGGGAAAAGTTTGCCTAT GTGCCATTTGGAGCTGGGCGTCATCGTTGTATTGGGGAAAATTTTGCCTATGTTCAAATT AAGACAATTTGGTCCACTATGCTTCGTTTATATGAATTTGATCTCATTGATGGATACTTT CCCACTGTGAATTATACAACTATGATTCACACCCCTGAAAACCCAGTTATCCGTTACAAA CGAAGATCAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001146152 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001146152.1, NP_001139624.1 |
| RefSeq Size | 2934 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 1595 |
| UniProt ID | Q16850 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228881 | CYP51A1 (Myc-DDK-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 3,990.00 |
|
| RC228881L3 | Lenti-ORF clone of CYP51A1 (Myc-DDK-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 5,890.00 |
|
| RC228881L4 | Lenti-ORF clone of CYP51A1 (mGFP-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 5,890.00 |
|
| RG228881 | CYP51A1 (tGFP-tagged) - Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 2 |
CNY 4,370.00 |
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