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Slingshot homolog 1 (SSH1) (NM_001161330) Human Untagged Clone
CAT＃: SC327172
SSH1 (untagged)-Human slingshot homolog 1 (Drosophila) (SSH1) transcript variant 2
CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SSH1L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327172 representing NM_001161330.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCTGGTGACCCTGCAGCGCTCGCCCACGCCCAGCGCCGCCTCCTCCTCGGCCAGCAACAGCGAG TTGGAGGCTGGCAGCGAAGAAGATCGAAAATTAAACCTCAGCTTAAGTGAGAGCTTTTTCATGGTGAAA GGCGCAGCCCTCTTCTTACAACAGGGAAGCAGCCCTCAAGGCCAGCGGAGTCTTCAGCACCCCCACAAG CATGCAGGTGATCTGCCTCAACATCTTCAGGTGATGATCAACCTTCTGCGTTGCGAAGACAGAATCAAG CTGGCAGTGCGCCTGGAGAGCGCCTGGGCGGACCGGGTCCGGTACATGGTGGTGGTGTACAGCAGCGGG CGCCAGGACACCGAGGAGAATATCTTGCTGGGAGTGGACTTTTCCAGTAAGGAAAGTAAAAGCTGCACC ATTGGGATGGTTCTCCGACTGTGGAGCGACACGAAAATCCACCTTGATGGAGATGGTGGGTTCAGCGTG AGCACAGCAGGAAGGATGCACATATTTAAGCCTGTGTCTGTCCAGGCCATGTGGTCTGCCCTGCAGGTG CTTCACAAGGCCTGCGAAGTGGCCCGGAGGCACAACTACTTCCCCGGGGGTGTAGCTCTCATCTGGGCT ACCTACTATGAGAGCTGCATCAGCTCCGAGCAGAGCTGCATCAACGAGTGGAACGCCATGCAGGACCTG GAGTCTACGCGGCCCGACTCCCCCGCGCTATTTGTGGACAAGCCCACTGAAGGGGAAAGGACCGAGCGC CTCATCAAAGCCAAGCTCCGAAGCATCATGATGAGCCAGGATCTAGAAAATGTGACTTCCAAAGAGATT CGTAATGAATTAGAGAAACAGATGAATTGTAACTTGAAGGAACTCAAGGAATTTATAGACAATGAGATG CTACTTATCTTGGGACAGATGGACAAGCCCTCCCTTATCTTCGATCATCTTTATCTCGGCTCTGAATGG AATGCATCCAATCTGGAGGAACTGCAGGGCTCAGGGGTTGATTACATTTTAAATGTTACCAGAGAAATC GATAATTTTTTTCCTGGCTTATTTGCATATCATAACATCCGAGTCTACGATGAAGAGACCACAGACCTC CTCGCCCACTGGAATGAAGCGTATCATTTTATAAACAAAGCGAAGAGGAACCATTCCAAGTGCCTGGTG CATTGCAAAATGGGCGTGAGTCGCTCGGCCTCCACAGTCATAGCCTATGCAATGAAGGAATTCGGCTGG CCTCTGGAAAAAGCATATAACTATGTAAAGCAGAAGCGCAGCATCACGCGCCCCAACGCGGGCTTTATG AGGCAGCTGTCTGAGTATGAAGGCATCTTGGATGCAAGCAAACAGCGGCACAACAAGCTGTGGCGTCAG CAGACAGACAGCAGCCTCCAGCAGCCTGTGGATGACCCTGCAGGACCTGGCGACTTCTTGCCAGAGACC CCAGATGGCACCCCGGAAAGCCAGCTGCCCTTCTTGGATGATGCCGCCCAGCCCGGCTTAGGGCCCCCC CTCCCCTGCTGTTTCCGGCGACTCTCAGACCCCCTTCTGCCTTCCCCTGAGGATGAAACTGGCAGCTTG GTCCACCTGGAGGATCCGGAGAGGGAGGCTCTGTTGGAGGAAGCTGCTCCACCTGCAGAGGTGCACAGG CCGGCCAGACAGCCCCAGCAAGGTTCCGGACTCTGTGAGAAGGATGTGAAGAAGAAACTAGAGTTTGGG AGTCCCAAAGGTCGGAGCGGCTCCTTGCTGCAGGTGGAGGAGACGGAAAGGGAGGAGGGCCTGGGAGCA GGGAGGTGGGGGCAGCTTCCAACCCAGCTCGATCAAAACCTGCTCAACTCGGAGAACCTAAACAACAAC AGCAAGAGGAGCTGTCCCAACGGCATGGAGGTAGGCAGAGCCCGGCCTGCAGGGTGGCACACCCCATCC CTTCCATCCCACTCTAATTGGCCTACCTCAGCCTCTGTAGTAGGGACTACAGGCACCCGCCACCACACC CAGCTGATTTTTTTCTATTGTCTCCTCTGGGCCCCCAGCTCCCATCTCCAGGGACCTGAGGGTTCTTTC ACAGGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001161330 |
| Insert Size | 2079 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001161330.1 |
| RefSeq Size | 2367 bp |
| RefSeq ORF | 2079 bp |
| Locus ID | 54434 |
| UniProt ID | Q8WYL5 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 77.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) differs at the 3' end compared to variant 1. This results in a shorter isoform (2, also known as SSH-1S) with a distinct C-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228537 | SSH1 (Myc-DDK-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 2 |
CNY 5,072.00 |
|
| RC228537L3 | Lenti-ORF clone of SSH1 (Myc-DDK-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 2 |
CNY 9,220.00 |
|
| RC228537L4 | Lenti-ORF clone of SSH1 (mGFP-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 2 |
CNY 9,220.00 |
|
| RG228537 | SSH1 (tGFP-tagged) - Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 2 |
CNY 8,080.00 |
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