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Neurofascin (NFASC) (NM_001005389) Human Untagged Clone
CAT＃: SC327141
NFASC (untagged)-Human neurofascin homolog (chicken) (NFASC) transcript variant 5
CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NEDCPMD; NF; NRCAML |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327141 representing NM_001005389.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCAGGCAGCCACCGCCGCCCTGGGTCCATGCAGCCTTCCTCCTCTGCCTCCTCAGTCTTGGCGGA GCCATCGAAATTCCTATGGATCCAAGCATTCAGAATGAGCTGACGCAGCCGCCAACCATCACCAAGCAG TCAGCGAAGGATCACATCGTGGACCCCCGTGATAACATCCTGATTGAGTGTGAAGCAAAAGGGAACCCT GCCCCCAGCTTCCACTGGACACGAAACAGCAGATTCTTCAACATCGCCAAGGACCCCCGGGTGTCCATG AGGAGGAGGTCTGGGACCCTGGTGATTGACTTCCGCAGTGGCGGGCGGCCGGAGGAATATGAGGGGGAA TATCAGTGCTTCGCCCGCAACAAATTTGGCACGGCCCTGTCCAATAGGATCCGCCTGCAGGTGTCTAAA TCTCCTCTGTGGCCCAAGGAAAACCTAGACCCTGTCGTGGTCCAAGAGGGCGCTCCTTTGACGCTCCAG TGCAACCCCCCGCCTGGACTTCCATCCCCGGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACC CAAGACAAACGTGTCTCTCAGGGCCATAACGGAGACCTATACTTCTCCAACGTGATGCTGCAGGACATG CAGACCGACTACAGTTGTAACGCCCGCTTCCACTTCACCCACACCATCCAGCAGAAGAACCCTTTCACC CTCAAGGTCCTCACCACCCGAGGAGTTGCAGAAAGAACACCAAGCTTCATGTATCCCCAGGGCACCGCG AGCAGCCAGATGGTGCTTCGTGGCATGGACCTCCTGCTGGAATGCATCGCCTCCGGGGTCCCAACACCA GACATCGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGATAAGGCCAAGTTTGAGAACTTTAATAAG GCCCTGCGTATCACAAATGTCTCTGAGGAAGACTCCGGGGAGTATTTCTGCCTGGCCTCCAACAAGATG GGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCCTACTGGCTGGACGAACCCAAGAAC CTTATTCTGGCTCCTGGCGAGGATGGGAGACTGGTGTGTCGAGCCAATGGAAACCCCAAACCCACTGTC CAGTGGATGGTGAATGGGGAACCTTTGCAATCGGCACCACCTAACCCAAACCGTGAGGTGGCCGGAGAC ACCATCATCTTCCGGGACACCCAGATCAGCAGCAGGGCTGTGTACCAGTGCAACACCTCCAACGAGCAT GGCTACCTGCTGGCCAACGCCTTTGTCAGTGTGCTGGATGTGCCGCCTCGGATGCTGTCGCCCCGGAAC CAGCTCATTCGAGTGATTCTTTACAACCGGACGCGGCTGGACTGCCCTTTCTTTGGGTCTCCCATCCCC ACACTGCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGCAACTACCATGTTTATGAGAAC GGCAGTCTGGAAATTAAGATGATCCGCAAAGAGGACCAGGGCATCTACACCTGTGTCGCCACCAACATC CTGGGCAAAGCTGAAAACCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACCGGATGCCCGAG GACCAGGTGGCCAGAAGGGGCACCACGGTGCAGCTGGAGTGTCGGGTGAAGCACGACCCCTCCCTGAAA CTCACCGTCTCCTGGCTGAAGGATGACGAGCCGCTCTATATTGGAAACAGGATGAAGAAGGAAGACGAC TCCCTGACCATCTTTGGGGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTGTCGCCAGCACCGAGCTA GACCAAGACCTGGCCAAGGCCTACCTCACCGTGCTAGGTAACTGCCCATGCTCACCCTGGCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001005389 |
| Insert Size | 1860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001005389.1 |
| RefSeq Size | 2527 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 23114 |
| UniProt ID | O94856 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 69.6 kDa |
| Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009] Transcript Variant: This variant (5) uses an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (5) has a substantially shorter and distinct C-terminus that lacks the fibronectin domains, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228506 | NFASC (Myc-DDK-tagged)-Human neurofascin (NFASC), transcript variant 5 |
CNY 4,608.00 |
|
| RC228506L3 | Lenti-ORF clone of NFASC (Myc-DDK-tagged)-Human neurofascin (NFASC), transcript variant 5 |
CNY 7,320.00 |
|
| RC228506L4 | Lenti-ORF clone of NFASC (mGFP-tagged)-Human neurofascin (NFASC), transcript variant 5 |
CNY 7,320.00 |
|
| RG228506 | NFASC (tGFP-tagged) - Human neurofascin (NFASC), transcript variant 5 |
CNY 5,990.00 |
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