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PDE1B (NM_001165975) Human Untagged Clone
CAT＃: SC327057
PDE1B (untagged)-Human phosphodiesterase 1B calmodulin-dependent (PDE1B) transcript variant 2
CNY 8,360.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-S-79p; PDE1B1; PDES1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327057 representing NM_001165975.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAAACCCTGTTCCTGTTCAGAGGAGCCACCTCCAGGGCCCCATTCTCAGGCTGCGCTACATGGTG AAGCAGTTGGAGAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCTTCTCTG CTGGAAGCCGTCTACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCTGCAGGAGCTGCGG TCAGATGCCGTGCCTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGGCCCGGGCCAAA GGCCGCCGAGCAGAGGAGAAGCCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCGTG GAACGGATGTTCCGGAGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTC AAGAACCTGGATCTCTGGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGG ACCATTGTTTTTGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTG ATGAGTTTCCTGGATGCCTTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCAC GCAGCCGATGTTACCCAGACAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAG ATTGAGCTCCTGGCCATCATCTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGC TTCCACATCCAGACCAAGTCAGAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCAC ATCAGCTCTGTTTTCCGATTGATGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAG TTTGTAGAACTCCGAGCCCTGGTCATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAA GTGAAGACCATGAAGACAGCCTTGCAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTG CTCCATGCTGCTGACATCAGCCACCCAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTC ATGGAGGAATTCTTCCGTCAGGGTGACAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGAC CGCACTTCCACTCTAGTGGCACAGTCTCAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCT GTGCTGACTGACGTGGCAGAGAAGAGTGTTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAG CCCAGCTTTCAGTGGCGCCAGCCCTCTCTGGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGC TTTCGTTCCACCTGGGTCAAGCGCATTCAGGAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGC ATCACCAACCAGATGTCCATTGACGAGCTGTCCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAA GATGAACACAACCAGAATGGGAATCTGGATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001165975 |
| Insert Size | 1551 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001165975.2 |
| RefSeq Size | 3107 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 5153 |
| UniProt ID | Q01064 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 59.1 kDa |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (2) contains a distinct 5' UTR, lacks part of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228422 | PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
CNY 3,840.00 |
|
| RC228422L3 | Lenti-ORF clone of PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
CNY 6,370.00 |
|
| RC228422L4 | Lenti-ORF clone of PDE1B (mGFP-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
CNY 6,370.00 |
|
| RG228422 | PDE1B (tGFP-tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
CNY 4,940.00 |
