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CERKL (NM_001160277) Human Untagged Clone
CAT＃: SC327056
CERKL (untagged)-Human ceramide kinase-like (CERKL) transcript variant 7
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RP26 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327056 representing NM_001160277.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCTGGAGGAGGCGCAGGAACCGGGTGAGTGCCCTGGAGGGCGGCCGGGAGGAAGAGGCGCCCCCG GAGGCTGCCGCTGTGCCTCCGGCGCTGTTAACGTCCCCGCAGCAGACGGAGGCGGCGGCCGAGCGGATT CTGCTCCGGGGCATCTTCGAGATCGGGAGGGACAGTTGTGACGTGGTGCTGAGCGAGCGAGCACTGCGG TGGCGGCCCATTCAGCCCGAGCGCCCGGCGGGTGATTCTAAGTATGACTTGCTATGTAAAGAAGAATTT ATTGAACTCAAAGACATATTCTCTGTGAAACTGAAACGGCGTTGTTCTGTTAAACAGCAGAGAAGTGGT ACTTTATTAGGTATCACACTCTTCATCTGCTTGAAAAAGGAACAAAATAAACTAAAGAATTCTACACTT GATCTTATTAATTTAAGTGAAGACCACTGTGACATATGGTTTAGACAGTTCAAGAAAATATTGGCAGTA ATGGAATATGAAGGGCACGCTCTGTCACTGCTTAAGGAATGTGAACTCCAGGGATTTGATGGTGGGCAT CGGAAACCATTGTTCGCAATACATTGGAGTGTACAGAGATTGTTCACTGGCATGCAAACGTTAGAACCA AGTGTTGTCTGTGTTGGTGGAGATGGATCTGCTAGCGAAGTAGCCCATGCTTTGCTTCTGAGAGCTCAG AAGAATGCTGGGATGGAAACAGACCGAATCCTGACTCCTGTCAGAGCACAGCTTCCACTTGGCTTAATA CCAGCAGGATCTACCAATGTATTGGCACATTCTCTTCATGGAGTTCCTCATGTGATAACTGCAACATTG CACATTATAATGGGGCATGTACAGCTGGTCGACGTCTGCACCTTCAGCACCGCTGGCAAGCTTCTTCGC TTTGGGTTCTCAGCCATGTTTGGCTTTGGTGGAAGAACTTTGGCTCTGGCAGAAAAATATCGATGGATG TCCCCTAACCAACGGAGAGATTTTGCTGTTGTTAAGGCACTGGCAAAACTTAAGGCAGAAGACTGTGAA ATATCATTTTTACCATTTAACAGCTCTGATGATGTGCAAGAAAGGAGGGCACAGGGATCTCCCAAATCT GACTGTAATGATCAATGGCAAATGATCCAGGGTCAGTTCTTGAATGTCAGCATTATGGCAATTCCTTGC CTGTGTTCAGTGGCACCTAGAGGCTTGGCACCTAATACCAGATTAAATAATGGAAGTATGGCTCTTATA ATTGCCCGAAACACTTCTCGGCCAGAATTTATAAAACACCTGAAAAGATATGCCAGTGTAAAAAATCAG TTCAATTTTCCATTTGTTGAGACTTACACTGTTGAGGAAGTAAAAGTTCATCCAAGGAATAATACTGGT GGATATAATCCAGAGGAGGAGGAGGATGAAACTGCTTCAGAAAATTGTTTCCCTTGGAATGTAGATGGT GACTTAATGGAAGTTGCATCAGAGGTCCATATTAGATTGCATCCAAGACTTATCAGTCTTTATGGAGGA AGCATGGAAGAAATGATTCCAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001160277 |
| Insert Size | 1545 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001160277.1 |
| RefSeq Size | 3172 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 375298 |
| UniProt ID | Q49MI3 |
| Protein Families | Druggable Genome |
| MW | 57.6 kDa |
| Gene Summary | This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010] Transcript Variant: This variant (7) lacks one alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (7) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228421 | CERKL (Myc-DDK-tagged)-Human ceramide kinase-like (CERKL), transcript variant 7 |
CNY 3,832.00 |
|
| RC228421L3 | Lenti-ORF clone of CERKL (Myc-DDK-tagged)-Human ceramide kinase-like (CERKL), transcript variant 7 |
CNY 6,460.00 |
|
| RC228421L4 | Lenti-ORF clone of CERKL (mGFP-tagged)-Human ceramide kinase-like (CERKL), transcript variant 7 |
CNY 6,460.00 |
|
| RG228421 | CERKL (tGFP-tagged) - Human ceramide kinase-like (CERKL), transcript variant 7 |
CNY 5,040.00 |
