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SRP54 (NM_001146282) Human Untagged Clone
CAT＃: SC327005
SRP54 (untagged)-Human signal recognition particle 54kDa (SRP54) transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SCN8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327005 representing NM_001146282.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCACCATTATCAATGAAGAGGTCTGCTATTGATCTTGAAGAGATGGCATCTGGTCTTAACAAAAGA AAAATGATTCAGCATGCTGTATTTAAAGAACTTGTGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACA CCCACTAAAGGAAAACAAAATGTGATTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGT TCAAAGCTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTCAGA GCAGGGGCTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTATGGAAGCTATACA GAAATGGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAAAATGAAAATTTTGAAATTATT ATTGTTGATACAAGTGGCCGCCACAAACAAGAAGACTCTTTGTTTGAAGAAATGCTTCAAGTTGCTAAT GCTATACAACCTGATAACATTGTTTATGTGATGGATGCCTCCATTGGGCAGGCTTGTGAAGCCCAGGCT AAGGCTTTTAAAGATAAAGTAGATGTAGCCTCAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGA GGTGGTGCACTCAGTGCAGTCGCTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATA GATGACTTTGAACCTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATTGAAGGA CTGATAGATAAAGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAGAAGTTGAAACATGGT CAGTTTACGTTGCGAGACATGTATGAGCAATTTCAAAATATCATGAAAATGGGCCCCTTCAGTCAGATC TTGGGGATGATCCCTGGTTTTGGGACAGATTTTATGAGCAAAGGAAATGAACAGGAGTCAATGGCAAGG CTAAAGAAATTAATGACAATAATGGATAGTATGAATGATCAAGAACTAGACAGTACGGATGGTGCCAAA GTTTTTAGTAAACAACCAGGAAGAATCCAAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTT CAAGAACTTTTGACACAATATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTT TTCAAAGGTGGCGACATGTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAACAAATGGCC AAAATGATGGATCCTAGGGTTCTTCATCACATGGGTGGTATGGCAGGACTTCAGTCAATGATGAGGCAG TTTCAACAGGGTGCTGCTGGCAACATGAAAGGCATGATGGGATTCAATAATATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001146282 |
| Insert Size | 1368 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001146282.1 |
| RefSeq Size | 2206 bp |
| RefSeq ORF | 1368 bp |
| Locus ID | 6729 |
| UniProt ID | P61011 |
| Protein Pathways | Protein export |
| MW | 50.4 kDa |
| Gene Summary | Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks a portion of the 5' coding region and uses a distinct translational start codon, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228370 | SRP54 (Myc-DDK-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 2 |
CNY 3,656.00 |
|
| RC228370L3 | Lenti ORF clone of Human signal recognition particle 54kDa (SRP54), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC228370L4 | Lenti ORF clone of Human signal recognition particle 54kDa (SRP54), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG228370 | SRP54 (tGFP-tagged) - Human signal recognition particle 54kDa (SRP54), transcript variant 2 |
CNY 4,370.00 |
