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NONO (NM_001145409) Human Untagged Clone
CAT＃: SC326482
NONO (untagged)-Human non-POU domain containing, octamer-binding (NONO), transcript variant 3, mRNA
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326482 representing NM_001145409.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGAGTAATAAAACTTTTAACTTGGAGAAGCAAAACCATACTCCAAGAAAGCATCATCAACATCAC CACCAGCAGCAGCACCACCAGCAGCAACAGCAGCAGCCGCCACCACCGCCAATACCTGCAAATGGGCAA CAGGCCAGCAGCCAAAATGAAGGCTTGACTATTGACCTGAAGAATTTTAGAAAACCAGGAGAGAAGACC TTCACCCAACGAAGCCGTCTTTTTGTGGGAAATCTTCCTCCCGACATCACTGAGGAAGAAATGAGGAAA CTATTTGAGAAATATGGAAAGGCAGGCGAAGTCTTCATTCATAAGGATAAAGGATTTGGCTTTATCCGC TTGGAAACCCGAACCCTAGCGGAGATTGCCAAAGTGGAGCTGGACAATATGCCACTCCGTGGAAAGCAG CTGCGTGTGCGCTTTGCCTGCCATAGTGCATCCCTTACAGTTCGAAACCTTCCTCAGTATGTGTCCAAC GAACTGCTGGAAGAAGCCTTTTCTGTGTTTGGCCAGGTAGAGAGGGCTGTAGTCATTGTGGATGATCGA GGAAGGCCCTCAGGAAAAGGCATTGTTGAGTTCTCAGGGAAGCCAGCTGCTCGGAAAGCTCTGGACAGA TGCAGTGAAGGCTCCTTCCTGCTAACCACATTTCCTCGTCCTGTGACTGTGGAGCCCATGGACCAGTTA GATGATGAAGAGGGACTTCCAGAGAAGCTGGTTATAAAAAACCAGCAATTTCACAAGGAACGAGAGCAG CCACCCAGATTTGCACAGCCTGGCTCCTTTGAGTATGAATATGCCATGCGCTGGAAGGCACTCATTGAG ATGGAGAAGCAGCAGCAGGACCAAGTGGACCGCAACATCAAGGAGGCTCGTGAGAAGCTGGAGATGGAG ATGGAAGCTGCACGCCATGAGCACCAGGTCATGCTAATGAGACAGGATTTGATGAGGCGCCAAGAAGAA CTTCGGAGGATGGAAGAGCTGCACAACCAAGAGGTGCAAAAACGAAAGCAACTGGAGCTCAGGCAGGAG GAAGAGCGCAGGCGCCGTGAAGAAGAGATGCGGCGGCAGCAAGAAGAAATGATGCGGCGACAGCAGGAA GGATTCAAGGGAACCTTCCCTGATGCGAGAGAGCAGGAGATTCGGATGGGTCAGATGGCTATGGGAGGT GCTATGGGCATAAACAACAGAGGTGCCATGCCCCCTGCTCCTGTGCCAGCTGGTACCCCAGCTCCTCCA GGACCTGCCACTATGATGCCGGATGGAACTTTGGGATTGACCCCACCAACAACTGAACGCTTTGGTCAG GCTGCTACAATGGAAGGAATTGGGGCAATTGGTGGAACTCCTCCTGCATTCAACCGTGCAGCTCCTGGA GCTGAATTTGCCCCAAACAAACGTCGCCGATACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145409 |
| Insert Size | 1416 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145409.1 |
| RefSeq Size | 3057 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 4841 |
| UniProt ID | Q15233 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 54.2 kDa |
| Gene Summary | This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226579 | NONO (Myc-DDK-tagged)-Human non-POU domain containing, octamer-binding (NONO), transcript variant 3 |
CNY 3,656.00 |
|
| RC226579L3 | Lenti ORF clone of Human non-POU domain containing, octamer-binding (NONO), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226579L4 | Lenti ORF clone of Human non-POU domain containing, octamer-binding (NONO), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG226579 | NONO (tGFP-tagged) - Human non-POU domain containing, octamer-binding (NONO), transcript variant 3 |
CNY 4,370.00 |
