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PAG3 (ASAP2) (NM_001135191) Human Untagged Clone
CAT＃: SC325379
ASAP2 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001135191, the custom clone sequence may differ by one or more nucleotides
ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCG CCCACGGCCTCCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATC GAGGAGGCTTTGGACGTGGACCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCA ATCAACAGCTCTGGGCTGGCTCACGTGGAAAATGAAGAGCAGTACACCCAGGCTCTGGAG AAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATTTAGGAAGTGCGTTCCTGAAG TTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCAGAATATGAAC AACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATA GAAAAGGAGAAAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGA GCGGAAATTGCCGAAGAGATGGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAG TATCTGCTGAAGGTCAACGAAATCAAGATTAAAAAGGGAGTAGATTTACTTCAGAATCTG ATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATGGACTCAAAGCCGTGGAAAGC CTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACAGGCCCAGGAT GAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA CAGAAAGAGGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAGCCTCAGGGA AACAAGGAACATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGACGGGATCCGA AAAGTGTGGCAGAAAAGGAAATGTTCAGTTAAAAATGGTTTTCTGACCATATCCCATGGT ACCGCTAACCGGCCTCCTGCAAAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAACCCT GAGGAGAAGAAGTGCTTTGACCTCATTTCACATGACAGAACTTACCACTTTCAAGCTGAA GATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAATAGCAAAGAAGAAGCTTTA AACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAAGAACTGACA AAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGTGACTGTGGG GCGCCAGATCCTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATCGAGTGTTCC GGAATCCACCGAGAGCTGGGGGTTCATTATTCCAGGATGCAGTCCCTGACCTTAGATGTA CTGGGAACATCTGAGCTGCTGCTCGCCAAGAATATTGGGAATGCAGGCTTTAATGAGATC ATGGAATGTTGCCTACCAGCTGAGGACTCAGTCAAACCCAACCCAGGCAGCGACATGAAT GCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAGATACGCAAGGAAGAAGCAC GCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGAGATATTTTT GGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCACTGGCCAAC GGACATGAGCCGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGATCGAACCTCT CTTCACATTGTAGACTTTTTAGTTCAGAACAGTGGGAACCTGGATAAACAGACAGGGAAA GGCAGCACAGCCCTGCACTACTGCTGCCTGACCGACAATGCCGAGTGCCTCAAGTTGCTC CTGCGGGGGAAGGCCTCCATCGAGATAGCAAACGAGTCAGGAGAGACTCCGCTGGACATT GCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGACCCAAGCCTTATCTGGAAGA TTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGACCTGGATGAA AGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAAGACCGGCCC ATCAGCTTCTACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCTTTGGCCAGA GATGCTGCAAACCTTGCCAAGGAGAAGCAGAGGGCTTTCATGCCCAGCATCTTGCAGAAT GAGACTTACGGAGCCCTCCTGAGTGGCAGCCCACCTCCCGCCCAGCCTGCAGCCCCCAGC ACCACCAGCGCCCCCCCGCTTCCTCCACGGAATGTTGGCAAAGATCCCCTGACCCCCACG CCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATGGAAGCCTTGAGCCAGCCGAGCAAG CCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCACCTCTGCCCCCACAGCCGCCCAGC CGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGACAAGTCCACCCCACTGACCAACAAA GGCCAACCGAGAGGACCTGTGGATCTCTCTGCAACGGAAGCTCTGGGTCCTCTGTCCAAT GCTATGGTCCTGCAGCCCCCTGCACCCATGCCTAGGAAGTCGCAGGCAACCAAGTTGAAG CCTAAGCGGGTGAAAGCGCTCTATAACTGTGTGGCTGACAACCCCGATGAGCTCACCTTC TCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAGGACCAGGAGTGGTGGATTGGCCAC ATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCGGTGTCATTTGTGCACTTTATCGCT GAC |
| Restriction Sites | Please inquire |
| ACCN | NM_001135191 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001135191.1, NP_001128663.1 |
| RefSeq Size | 5589 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 8853 |
| UniProt ID | O43150 |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
| Gene Summary | This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is lacking a 45-aa portion of the proline-rich region, compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226279 | ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 7,130.00 |
|
| RC226279L3 | Lenti-ORF clone of ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 9,030.00 |
|
| RC226279L4 | Lenti-ORF clone of ASAP2 (mGFP-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 9,030.00 |
|
| RG226279 | ASAP2 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2 |
CNY 7,890.00 |
