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SPART (NM_001142294) Human Untagged Clone
CAT＃: SC325265
SPG20 (untagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4
CNY 4,888.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPG20; TAHCCP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325265 representing NM_001142294.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAAATTAAGATCATCAGAGAAGCATATAAGAAGGCC TTTTTATTTGTTAACAAAGGTCTGAATACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTAT AAGCAAGGAATAGGACACCTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTCCT GGGTGGGAATCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCTG GAAATTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAGTTATATCCA GAATTTCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTTAGTTCAGCTCCTCAGCAT GCTGAAGTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCAGTTGCTGCACCTGCTTCTCTGTCTTTA CCATCACAAAGTTGTCCAGCAGAAGCTCCTCCTGCTTATACTCCTCAAGCTGCTGAAGGTCACTACACT GTATCCTATGGAACAGATTCTGGGGAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAG CCACCGCCTCTTGAGACCTTAGGGCTGGATGCAGATGAATTGATTTTGATACCAAATGGAGTACAGATT TTTTTTGTAAATCCTGCAGGGGAGGTTAGTGCACCTTCGTATCCTGGGTACCTTCGAATTGTGAGGTTT TTGGATAATTCTCTCGATACGGTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTGTGACTGGTTATAT CCTCTAGTTCCTGATAGATCTCCGGTTCTGAAATGTACTGCGGGAGCCTACATGTTTCCTGATACAATG CTACAAGCAGCAGGATGCTTTGTGGGGGTCGTCCTGTCCTCTGAGTTACCAGAGGATGATAGAGAGCTC TTTGAGGATCTGTTAAGGCAAATGTCTGACCTTCGGCTCCAGGCCAACTGGAACAGAGCAGAAGAAGAA AATGAATTCCAAATCCCTGGAAGAACTAGACCCTCCTCTGACCAACTAAAAGAAGCCTCTGGCACTGAT GTGAAACAGTTGGACCAAGGCAATAAGGATGTACGTCATAAAGGAAAACGTGGAAAAAGGGCTAAAGAT ACTTCAAGTGAAGAAGTTAACCTGAGTCACATTGTACCATGTGAGCCAGTTCCAGAAGAAAAGCCAAAA GAATTACCTGAATGGAGTGAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTCCTGGGTGAGTTGGGGT TTAGTCAAAGGTGCTGAGATTACTGGTAAGGCAATCCAGAAAGGTGCTTCTAAACTCCGAGAGCGGATT CAACCAGAAGAAAAACCCGTGGAAGTTAGTCCAGCTGTCACCAAGGGACTTTATATAGCGAAGCAAGCT ACAGGAGGAGCAGCAAAAGTCAGTCAGTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGA AAAGAACTAGCTCCACATGTCAAGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAAA GATGGGAAATCTCCTCTGGATGGTGCTATGGTTGTAGCAGCAAGTAGTGTTCAAGGATTTTCAACTGTC TGGCAAGGATTGGAATGTGCAGCTAAATGCATCGTTAACAATGTTTCAGCAGAAACTGTACAAACTGTC AGATACAAATACGGATATAATGCAGGAGAAGCTACCCACCATGCGGTGGATTCTGCGGTCAATGTTGGC GTAACTGCCTACAATATTAACAACATTGGTATCAAAGCAATGGTGAAGAAAACTGCAACACAAACAGGA CACACTCTCCTTGAGGACTATCAGATAGTTGATAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAAT GTCAACGTGAGAGGGGAGAAGGATGAGCAGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142294 |
| Insert Size | 2001 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142294.1 |
| RefSeq Size | 4838 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 23111 |
| UniProt ID | Q8N0X7 |
| MW | 72.8 kDa |
| Gene Summary | This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008] Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227155 | SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 4,960.00 |
|
| RC227155L3 | Lenti-ORF clone of SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 5,890.00 |
|
| RC227155L4 | Lenti-ORF clone of SPG20 (mGFP-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 5,890.00 |
|
| RG227155 | SPG20 (tGFP-tagged) - Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 4,370.00 |
