NCOA4 (NM_001145263) Human Untagged Clone

CAT#: SC325236

NCOA4 (untagged)-Human nuclear receptor coactivator 4 (NCOA4), transcript variant 4



  "NM_001145263" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
NCOA4 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ARA70; ELE1; PTC3; RFG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC325236 representing NM_001145263.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAATACCTTCCAAGACCAGAGTGGCAGCTCCAGTAATAGAGAACCCCTTTTGAGGTGTAGTGATGCA
CGGAGGGACTTGGAGCTTGCTATTGGTGGAGTTCTCCGGGCTGAACAGCAAATTAAAGATAACTTGCGA
GAGGTCAAAGCTCAGATTCACAGTTGCATAAGCCGTCACCTGGAATGTCTTAGAAGCCGTGAGGTATGG
CTGTATGAACAGGTGGACCTTATTTATCAGCTTAAAGAGGAGACACTTCAACAGCAGGCTCAGCAGCTC
TACTCGTTATTGGGCCAGTTCAATTGTCTTACTCATCAACTGGAGTGTACCCAAAACAAAGATCTAGCC
AATCAAGTCTCTGTGTGCCTGGAGAGACTGGGCAGTTTGACCCTTAAGCCTGAAGATTCAACTGTCCTG
CTCTTTGAAGCTGACACAATTACTCTGCGCCAGACCATCACCACATTTGGGTCTCTCAAAACCATTCAA
ATTCCTGAGCACTTGATGGCTCATGCTAGTTCAGCAAATATTGGGCCCTTCCTGGAGAAGAGAGGCTGT
ATCTCCATGCCAGAGCAGAAGTCAGCATCCGGTATTGTAGCTGTCCCTTTCAGCGAATGGCTCCTTGGA
AGCAAACCTGCCAGTGGTTATCAAGCTCCTTACATACCCAGCACCGACCCCCAGGACTGGCTTACCCAA
AAGCAGACCTTGGAGAACAGTCAGACTTCTTCCAGAGCCTGCAATTTCTTCAATAATGTCGGGGGAAAC
CTAAAGGGCTTAGAAAACTGGCTCCTCAAGAGTGAAAAATCAAGTTATCAAAAGTGTAACAGCCATTCC
ACTACTAGTTCTTTCTCCATTGAAATGGAAAAGGTTGGAGATCAAGAGCTTCCTGATCAAGATGAGATG
GACCTATCAGATTGGCTAGTGACTCCCCAGGAATCCCATAAGCTGCGGAAGCCTGAGAATGGCAGTCGT
GAAACCAGTGAGAAGTTTAAGCTCTTATTCCAGTCCTATAATGTGAATGATTGGCTTGTCAAGACTGAC
TCCTGTACCAACTGTCAGGGAAACCAGCCCAAAGGTGTGGAGATTGAAAACCTGGGCAATCTGAAGTGC
CTGAATGACCACTTGGAGGCCAAGAAACCATTGTCCACCCCCAGCATGGTTACAGAGGATTGGCTTGTC
CAGAACCATCAGGACCCATGTAAGGTAGAGGAGGTGTGCAGAGCCAATGAGCCCTGCACAAGCTTTGCA
GAGTGTGTGTGTGATGAGAATTGTGAGAAGGAGGCTCTGTATAAGTGGCTTCTGAAGAAAGAAGGAAAG
GATAAAAATGGGATGCCTGTGGAACCCAAACCTGAGCCTGAGAAGCATAAAGATTCCCTGAATATGTGG
CTCTGTCCTAGAAAAGAAGTAATAGAACAAACTAAAGCACCAAAGGCAATGACTCCTTCTAGAATTGCT
GATTCCTTCCAAGTCATAAAGAACAGCCCCTTGTCGGAGTGGCTTATCAGGCCCCCATACAAAGAAGGA
AGTCCCAAGGAAGTGCCTGGTACTGAAGACAGAGCTGGCAAACAGAAGTTTAAAAGCCCCATGAATACT
TCCTGGTGTTCCTTTAACACAGCTGACTGGGTCCTGCCAGGAAAGAAGATGGGCAACCTCAGCCAGTTA
TCTTCTGGAGAAGACAAGTGGCTGCTTCGAAAGAAGGCCCAGGAAGTATTACTTAATTCACCTCTACAG
GAGGAACATAACTTCCCCCCAGACCATTATGGCCTCCCTGCAGTTTGTGATCTCTTTGCCTGTATGCAG
CTTAAAGTTGATAAAGAGAAGTGGTTATATCGAACTCCTCTACAGATGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001145263
Insert Size 1845 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001145263.1
RefSeq Size 3562 bp
RefSeq ORF 1845 bp
Locus ID 8031
UniProt ID Q13772
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Pathways in cancer, Thyroid cancer
MW 69.7 kDa
Gene Summary This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]
Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region and uses a downstream start codon, compared to variant 1. This variant (4) also lacks an exon in the 3' coding region, which results in a frameshift and use of a downstream stop codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus and a shorter, distinct C-terminus compared to isoform 1. Variants 3, 4, and 5 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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