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P4HA1 (NM_001142595) Human Untagged Clone
CAT＃: SC325169
P4HA1 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3
CNY 7,220.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | P4HA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325169 representing NM_001142595.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACT TCAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATT AAGGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACA GCGACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAAT ACTGAGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACC ATTCAGAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAG GATACCTACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTT CTAACGGCTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAA CTGTGGATGGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTT CTAGATTATTTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAG CTTCTTGAACTAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCT AAAGAAAAAGATGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAA GGGGTTGCTGTGGATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAA ATGACCCCTCGGAGACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATT CTGGCTCCAGCTAAACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCT GATGCAGAAATTGAAATCGTCAAAGACCTAGCAAAACCAAGGCTGAGGCGAGCCACCATTTCAAACCCA ATAACAGGAGACTTGGAGACGGTACATTACAGAATTAGCAAAAGTGCCTGGCTCTCTGGCTATGAAAAT CCTGTGGTGTCTCGAATTAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAA TTACAGGTAGCAAATTATGGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAG CCAGATGCTTTCAAAGAGCTGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTG TCTGCAGGAGGAGCCACTGTTTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTT TTCTGGTATAATCTGTTTGCCAGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTA GTTGGCAACAAATGGGTATCCAATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACG TTGTCAGAATTGGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142595 |
| Insert Size | 1605 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142595.1 |
| RefSeq Size | 2953 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 5033 |
| UniProt ID | P13674 |
| Protein Families | Druggable Genome, P450 |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
| MW | 61 kDa |
| Gene Summary | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate in-frame exon, compared to variant 1. It encodes isoform 2, which is the same length as isoform 1. Variants 2 and 3 both encode isoform 2. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227800 | P4HA1 (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3 |
CNY 3,976.00 |
|
| RC227800L1 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3, Myc-DDK-tagged |
CNY 6,376.00 |
|
| RC227800L2 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC227800L3 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC227800L4 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG227800 | P4HA1 (tGFP-tagged) - Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3 |
CNY 4,370.00 |
