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LEPREL1 (P3H2) (NM_001134418) Human Untagged Clone
CAT＃: SC325166
LEPREL1 (untagged)-Human leprecan-like 1 (LEPREL1), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LEPREL1; MCVD; MLAT4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325166 representing NM_001134418.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAATGCAGCAGAACATTGAGAATTACAGGGCGACAGCTGGTGTTGAAGCATTGCAGTTGGTAGAC AGAGAAGCCAAGCCACACATGGAGAGTTACAATGCAGGAGTTAAACATTATGAGGCTGATGACTTTGAG ATGGCTATCAGGCACTTCGAACAAGCCTTAAGAGAATATTTCGTTGAAGATACAGAATGCCGGACCCTA TGTGAGGGGCCTCAGAGATTTGAAGAATATGAGTATTTAGGGTATAAGGCTGGTCTGTATGAAGCTATT GCAGATCACTACATGCAGGTGCTTGTTTGTCAGCATGAATGTGTGAGGGAACTTGCCACCCGCCCTGGC CGCCTCTCTCCCATCGAGAATTTTCTTCCTCTGCACTATGATTACCTACAGTTTGCCTACTATCGAGTT GGTGAGTATGTGAAAGCCCTGGAGTGTGCCAAAGCCTATCTTCTATGCCATCCAGATGATGAGGATGTC CTAGACAATGTGGATTACTATGAGAGTCTGCTGGATGATAGCATTGACCCGGCATCCATTGAGGCCAGA GAGGATTTAACAATGTTTGTGAAACGTCATAAGCTGGAGTCTGAGCTGATAAAATCAGCTGCAGAAGGT CTGGGGTTTTCATACACTGAACCGAATTATTGGATCAGATATGGAGGACGACAGGATGAGAATCGGGTC CCTTCAGGAGTGAACGTAGAGGGAGCAGAAGTTCATGGATTCTCAATGGGAAAAAAGCTATCACCCAAG ATAGATCGAGACCTAAGAGAAGGTGGTCCTCTACTCTATGAGAACATCACATTCGTCTACAACTCGGAG CAGCTGAACGGGACTCAGCGGGTTCTCCTGGATAACGTCCTGTCGGAAGAACAGTGCCGGGAGCTCCAC AGCGTGGCCAGTGGAATCATGCTTGTTGGTGATGGATACAGAGGAAAAACTTCACCCCATACACCCAAT GAAAAGTTTGAAGGTGCAACTGTCCTGAAAGCACTCAAATCTGGTTATGAAGGTCGAGTCCCACTGAAG AGCGCTCGTCTGTTTTATGACATCAGCGAAAAGGCTCGAAGGATTGTAGAATCTTATTTTATGCTGAAC TCAACTCTGTATTTTTCCTATACACACATGGTCTGCCGAACAGCCCTGTCTGGTCAGCAGGATAGAAGA AATGACCTCAGTCATCCCATCCATGCTGACAACTGTTTGTTGGATCCAGAGGCCAACGAATGCTGGAAG GAGCCTCCTGCTTACACATTTCGAGACTATAGTGCTCTCCTATATATGAATGATGACTTTGAAGGAGGA GAATTCATATTCACAGAGATGGATGCTAAGACTGTGACTGCCTCTATAAAACCAAAATGTGGGCGCATG ATCAGCTTCTCATCTGGAGGAGAGAACCCTCATGGGGTGAAGGCAGTCACCAAGGGAAAGAGGTGTGCT GTGGCTCTGTGGTTCACCTTGGACCCACTTTATAGAGAATTGGAGCGAATACAGGCTGATGAAGTGATT GCAATTCTGGATCAAGAACAGCAAGGGAAGCATGAACTGAATATCAACCCTAAAGATGAGCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001134418 |
| Insert Size | 1584 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001134418.1 |
| RefSeq Size | 3082 bp |
| RefSeq ORF | 1584 bp |
| Locus ID | 55214 |
| UniProt ID | Q8IVL5 |
| MW | 60.4 kDa |
| Gene Summary | This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225901 | LEPREL1 (Myc-DDK-tagged)-Human leprecan-like 1 (LEPREL1), transcript variant 2 |
CNY 3,928.00 |
|
| RC225901L3 | Lenti-ORF clone of LEPREL1 (Myc-DDK-tagged)-Human leprecan-like 1 (LEPREL1), transcript variant 2 |
CNY 5,890.00 |
|
| RC225901L4 | Lenti-ORF clone of LEPREL1 (mGFP-tagged)-Human leprecan-like 1 (LEPREL1), transcript variant 2 |
CNY 5,890.00 |
|
| RG225901 | LEPREL1 (tGFP-tagged) - Human leprecan-like 1 (LEPREL1), transcript variant 2 |
CNY 4,370.00 |
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