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PUF60 (NM_001136033) Human Untagged Clone
CAT＃: SC325151
PUF60 (untagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant c
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FIR; RoBPI; SIAHBP1; VRJS |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001136033, the custom clone sequence may differ by one or more nucleotides
ATGGAGAACGGGCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAG CAGGAGGCCCTTCAGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTG GTGAAGCAGACCATCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCAGCAGTG ACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGATGGCGGCTCAGCGGCAGCGG GCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGGGGGAGGAC ACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGAC TCCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCA CAGCTGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGC AGACCCAGCAACATAGGGCAGGCCCAGCCCATCATAGACCAGTTGGCTGAGGAGGCACGG GCCTTCAACCGCATCTACGTGGCCTCTGTGCACCAGGACCTCTCAGACGATGACATCAAG AGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACTGGCCCGGGACCCCACAACT GGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCCCAAGATGCT GTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAAGGCTGTC ACACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGCCGCTGCT GTGGCAGCTGCTGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGGAGCAGCG GTGCTGGGTACCCTGGGCACACCTGGACTGGTGTCCCCAGCACTGACCCTGGCCCAGCCC CTGGGCACTTTGCCCCAGGCTGTCATGGCTGCCCAGGCACCTGGAGTCATCACAGGTGTG ACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGTGGGAGTGGTGAACCCCATC CTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAAGAAGAGGAG CTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAGCATCTCG GGCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTCTACAGTG ATGGTTCTGCGCAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGGGGAGGTG ACAGAGGAGTGTGGCAAGTTCGGGGCCGTGAACCGCGTCATCATCTACCAAGAGAAACAA GGCGAGGAGGAGGATGCAGAAATCATTGTCAAGATCTTTGTGGAGTTTTCCATAGCCTCT GAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTTTGCTGGCCGCAAGGTGGTG GCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCG |
| Restriction Sites | Please inquire |
| ACCN | NM_001136033 |
| Insert Size | 1967 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001136033.1, NP_001129505.1 |
| RefSeq Size | 1967 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 22827 |
| UniProt ID | Q9UHX1 |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start site, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227333 | PUF60 (Myc-DDK-tagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant c |
CNY 5,130.00 |
|
| RC227333L3 | Lenti-ORF clone of PUF60 (Myc-DDK-tagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant c |
CNY 7,030.00 |
|
| RC227333L4 | Lenti-ORF clone of PUF60 (mGFP-tagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant c |
CNY 7,030.00 |
|
| RG227333 | PUF60 (tGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant c |
CNY 5,610.00 |
