Calcineurin A (PPP3CA) (NM_001130691) Human Untagged Clone

CAT#: SC325147

PPP3CA (untagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2



  "NM_001130691" in other vectors (4)

CNY 7,220.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (4)
PPP3CA mouse monoclonal antibody,clone OTI5C6
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; IECEE; IECEE1; PPP2B
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001130691 edited
AGAAAATAAATAAATAACCACCACACACGCGCAGCCCGGAGCGAGTCGGCGGGGCTGGCG
GCAGCGGCGGAGGAGGAGTGAAGGCGGCGGCGGCGGAGGAGGGACGCGCGGAAAAGGCAG
CAACTTTAAAGCCAGCTCAGAGCCTAGACCTCCAGCCGAGTGGTTTGCAGCGCGGCGGCG
GCGGCGGCGGCGGCGGCGGCGGCGTTGAGTGTCTGGCCCGCCGGTCCGGTCGGGGTGTGC
AGTCGGACGGACGAGCAGCGCGTCGCTGTCCTCCGGCAGCTGGAGATGTCCGAGCCCAAG
GCAATTGATCCCAAGTTGTCGACGACCGACAGGGTGGTGAAAGCTGTTCCATTTCCTCCA
AGTCACCGGCTTACAGCAAAAGAAGTGTTTGATAATGATGGAAAACCTCGTGTGGATATC
TTAAAGGCGCATCTTATGAAGGAGGGAAGGCTGGAAGAGAGTGTTGCATTGAGAATAATA
ACAGAGGGTGCATCAATTCTTCGACAGGAAAAAAATTTGCTGGATATTGATGCGCCAGTC
ACTGTTTGTGGGGACATTCATGGACAATTCTTTGATTTGATGAAGCTCTTTGAAGTCGGG
GGATCTCCTGCCAACACTCGCTACCTCTTCTTAGGGGACTATGTTGACAGAGGGTACTTC
AGTATTGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTCTACCCCAAAACACTGTTT
TTACTTCGTGGAAATCATGAATGTAGACATCTAACAGAGTATTTCACATTTAAACAAGAA
TGTAAAATAAAGTATTCAGAACGCGTATATGATGCCTGTATGGATGCCTTTGACTGCCTT
CCCCTGGCTGCCCTGATGAACCAACAGTTCCTGTGTGTGCATGGTGGTTTGTCTCCAGAG
ATTAACACTTTAGATGATATCAGAAAATTAGACCGATTCAAAGAACCACCTGCATATGGA
CCTATGTGTGATATCCTGTGGTCAGACCCCCTGGAAGATTTTGGAAATGAGAAGACTCAG
GAACATTTCACTCACAACACAGTCAGGGGGTGTTCATACTTCTACAGTTACCCGGCTGTA
TGTGAATTCTTACAGCACAATAACTTGTTATCTATACTCCGAGCCCACGAAGCCCAAGAT
GCAGGGTACCGCATGTACAGGAAAAGCCAAACAACAGGCTTCCCTTCTCTAATTACAATT
TTTTCAGCACCAAATTACTTAGATGTATACAATAACAAAGCTGCAGTATTGAAGTATGAG
AACAATGTTATGAATATCAGGCAATTCAACTGTTCTCCTCATCCATACTGGCTTCCAAAT
TTCATGGATGTTTTTACTTGGTCCCTTCCATTTGTTGGGGAAAAAGTGACTGAGATGCTG
GTAAATGTCCTCAACATCTGCTCAGATGATGAACTAGGGTCAGAAGAAGATGGATTTGAT
GGTGCAACAGCTGCAGCCCGGAAAGAGGTGATAAGGAACAAGATCCGAGCAATAGGCAAA
ATGGCCAGAGTGTTCTCAGTGCTCAGAGAAGAGAGTGAGAGTGTGCTGACGCTGAAAGGC
TTGACCCCAACTGGCATGCTCCCCAGCGGAGTACTTTCTGGAGGGAAGCAAACCCTGCAA
AGCGCTATCAAAGGATTTTCACCACAACATAAGATCACTAGCTTCGAGGAAGCCAAGGGC
TTAGACCGAATTAATGAGAGGATGCCGCCTCGCAGAGATGCCATGCCCTCTGACGCCAAC
CTTAACTCCATCAACAAGGCTCTCACCTCAGAGACTAACGGCACGGACAGCAATGGCAGT
AATAGCAGCAATATTCAGTGACCACTTCCTGTTCACTTTTTTTTTTTTTTTTTTTTTTTT
TTTTGAGCTGCGGGGCATGATGGGGATTGCTGCATATCAGCAGTTGGATGTTCTTGCCTC
TGACAGTAGCTTATTTGCTCTGGGGGCCAGGAATTGGATTCAGTTTACACTATCATTAAA
AAAGAGGGAGAGAGATAATAAACTATATTTTGGTGGGGATGGTGATTAAACACCTCTTTT
GGGTATGCCTTTTAAAAATGCTTATAGAGAAAAAAAATTTTAAAAAGAAAGCTAATGCTA
GTATATACTGCAATGTTAGGGGAATGAACATGTTTTCCTACTGCATTGGGGACTTCTAGA
TAGGTTAATGAAAGGCCTTTTATTCTGTTACTGGACATGAAAACTTTGTCTAATTTCTTA
CTCTATTGTACGTTTACAGTCGCAGCACTAAAAATGGATGACATCAAACATTTTTAACAA
AATGATGTACAAACTAAGGACTATTTATTGATAATGTTTTGCTACTCTTGTCAGACAATG
GCTATAAACTGAATTAGGCAGTCTTAAAAAAAAAAAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_001130691
Insert Size 2400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001130691.1.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001130691.1, NP_001124163.1
RefSeq Size 4646 bp
RefSeq ORF 1536 bp
Locus ID 5530
UniProt ID Q08209
Protein Families Druggable Genome, Phosphatase
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
Gene Summary Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 2), compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...