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Calcineurin A (PPP3CA) (NM_001130691) Human Untagged Clone
CAT＃: SC325147
PPP3CA (untagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; IECEE; IECEE1; PPP2B |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001130691 edited
AGAAAATAAATAAATAACCACCACACACGCGCAGCCCGGAGCGAGTCGGCGGGGCTGGCG GCAGCGGCGGAGGAGGAGTGAAGGCGGCGGCGGCGGAGGAGGGACGCGCGGAAAAGGCAG CAACTTTAAAGCCAGCTCAGAGCCTAGACCTCCAGCCGAGTGGTTTGCAGCGCGGCGGCG GCGGCGGCGGCGGCGGCGGCGGCGTTGAGTGTCTGGCCCGCCGGTCCGGTCGGGGTGTGC AGTCGGACGGACGAGCAGCGCGTCGCTGTCCTCCGGCAGCTGGAGATGTCCGAGCCCAAG GCAATTGATCCCAAGTTGTCGACGACCGACAGGGTGGTGAAAGCTGTTCCATTTCCTCCA AGTCACCGGCTTACAGCAAAAGAAGTGTTTGATAATGATGGAAAACCTCGTGTGGATATC TTAAAGGCGCATCTTATGAAGGAGGGAAGGCTGGAAGAGAGTGTTGCATTGAGAATAATA ACAGAGGGTGCATCAATTCTTCGACAGGAAAAAAATTTGCTGGATATTGATGCGCCAGTC ACTGTTTGTGGGGACATTCATGGACAATTCTTTGATTTGATGAAGCTCTTTGAAGTCGGG GGATCTCCTGCCAACACTCGCTACCTCTTCTTAGGGGACTATGTTGACAGAGGGTACTTC AGTATTGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTCTACCCCAAAACACTGTTT TTACTTCGTGGAAATCATGAATGTAGACATCTAACAGAGTATTTCACATTTAAACAAGAA TGTAAAATAAAGTATTCAGAACGCGTATATGATGCCTGTATGGATGCCTTTGACTGCCTT CCCCTGGCTGCCCTGATGAACCAACAGTTCCTGTGTGTGCATGGTGGTTTGTCTCCAGAG ATTAACACTTTAGATGATATCAGAAAATTAGACCGATTCAAAGAACCACCTGCATATGGA CCTATGTGTGATATCCTGTGGTCAGACCCCCTGGAAGATTTTGGAAATGAGAAGACTCAG GAACATTTCACTCACAACACAGTCAGGGGGTGTTCATACTTCTACAGTTACCCGGCTGTA TGTGAATTCTTACAGCACAATAACTTGTTATCTATACTCCGAGCCCACGAAGCCCAAGAT GCAGGGTACCGCATGTACAGGAAAAGCCAAACAACAGGCTTCCCTTCTCTAATTACAATT TTTTCAGCACCAAATTACTTAGATGTATACAATAACAAAGCTGCAGTATTGAAGTATGAG AACAATGTTATGAATATCAGGCAATTCAACTGTTCTCCTCATCCATACTGGCTTCCAAAT TTCATGGATGTTTTTACTTGGTCCCTTCCATTTGTTGGGGAAAAAGTGACTGAGATGCTG GTAAATGTCCTCAACATCTGCTCAGATGATGAACTAGGGTCAGAAGAAGATGGATTTGAT GGTGCAACAGCTGCAGCCCGGAAAGAGGTGATAAGGAACAAGATCCGAGCAATAGGCAAA ATGGCCAGAGTGTTCTCAGTGCTCAGAGAAGAGAGTGAGAGTGTGCTGACGCTGAAAGGC TTGACCCCAACTGGCATGCTCCCCAGCGGAGTACTTTCTGGAGGGAAGCAAACCCTGCAA AGCGCTATCAAAGGATTTTCACCACAACATAAGATCACTAGCTTCGAGGAAGCCAAGGGC TTAGACCGAATTAATGAGAGGATGCCGCCTCGCAGAGATGCCATGCCCTCTGACGCCAAC CTTAACTCCATCAACAAGGCTCTCACCTCAGAGACTAACGGCACGGACAGCAATGGCAGT AATAGCAGCAATATTCAGTGACCACTTCCTGTTCACTTTTTTTTTTTTTTTTTTTTTTTT TTTTGAGCTGCGGGGCATGATGGGGATTGCTGCATATCAGCAGTTGGATGTTCTTGCCTC TGACAGTAGCTTATTTGCTCTGGGGGCCAGGAATTGGATTCAGTTTACACTATCATTAAA AAAGAGGGAGAGAGATAATAAACTATATTTTGGTGGGGATGGTGATTAAACACCTCTTTT GGGTATGCCTTTTAAAAATGCTTATAGAGAAAAAAAATTTTAAAAAGAAAGCTAATGCTA GTATATACTGCAATGTTAGGGGAATGAACATGTTTTCCTACTGCATTGGGGACTTCTAGA TAGGTTAATGAAAGGCCTTTTATTCTGTTACTGGACATGAAAACTTTGTCTAATTTCTTA CTCTATTGTACGTTTACAGTCGCAGCACTAAAAATGGATGACATCAAACATTTTTAACAA AATGATGTACAAACTAAGGACTATTTATTGATAATGTTTTGCTACTCTTGTCAGACAATG GCTATAAACTGAATTAGGCAGTCTTAAAAAAAAAAAAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001130691 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001130691.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130691.1, NP_001124163.1 |
| RefSeq Size | 4646 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 5530 |
| UniProt ID | Q08209 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| Gene Summary | Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 2), compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225881 | PPP3CA (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2 |
CNY 5,130.00 |
|
| RC225881L3 | Lenti-ORF clone of PPP3CA (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2 |
CNY 7,030.00 |
|
| RC225881L4 | Lenti-ORF clone of PPP3CA (mGFP-tagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2 |
CNY 7,030.00 |
|
| RG225881 | PPP3CA (tGFP-tagged) - Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2 |
CNY 5,610.00 |
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