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Properdin (CFP) (NM_001145252) Human Untagged Clone
CAT＃: SC325124
CFP (untagged)-Human complement factor properdin (CFP), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BFD; PFC; PFD; PROPERDIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325124 representing NM_001145252.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCACAGAGGGAGCGCAGGCCCCTCGATTGTTGCTGCCGCCGCTGCTCCTGCTGCTCACCCTGCCA GCCACAGGCTCAGACCCCGTGCTCTGCTTCACCCAGTATGAAGAATCCTCCGGCAAGTGCAAGGGCCTC CTGGGGGGTGGTGTCAGCGTGGAAGACTGCTGTCTCAACACTGCCTTTGCCTACCAGAAACGTAGTGGT GGGCTCTGTCAGCCTTGCAGGTCCCCACGATGGTCCCTGTGGTCCACATGGGCCCCCTGTTCGGTGACG TGCTCTGAGGGCTCCCAGCTGCGGTACCGGCGCTGTGTGGGCTGGAATGGGCAGTGCTCTGGAAAGGTG GCACCTGGGACCCTGGAGTGGCAGCTCCAGGCCTGTGAGGACCAGCAGTGCTGTCCTGAGATGGGCGGC TGGTCTGGCTGGGGGCCCTGGGAGCCTTGCTCTGTCACCTGCTCCAAAGGGACCCGGACCCGCAGGCGA GCCTGTAATCACCCTGCTCCCAAGTGTGGGGGCCACTGCCCAGGACAGGCACAGGAATCAGAGGCCTGT GACACCCAGCAGGTCTGCCCCACACACGGGGCCTGGGCCACCTGGGGCCCCTGGACCCCCTGCTCAGCC TCCTGCCACGGTGGACCCCACGAACCTAAGGAGACACGAAGCCGCAAGTGTTCTGCACCTGAGCCCTCC CAGAAACCTCCTGGGAAGCCCTGCCCGGGGCTAGCCTACGAGCAGCGGAGGTGCACCGGCCTGCCACCC TGCCCAGTGGCTGGGGGCTGGGGGCCTTGGGGCCCTGTGAGCCCCTGCCCTGTGACCTGTGGCCTGGGC CAGACCATGGAACAACGGACGTGCAATCACCCTGTGCCCCAGCATGGGGGCCCCTTCTGTGCTGGCGAT GCCACCCGGACCCACATCTGCAACACAGCTGTGCCCTGCCCTGTGGATGGGGAGTGGGACTCGTGGGGG GAGTGGAGCCCCTGTATCCGACGGAACATGAAGTCCATCAGCTGTCAAGAAATCCCGGGCCAGCAGTCA CGCGGGAGGACCTGCAGGGGCCGCAAGTTTGACGGACATCGATGTGCCGGGCAACAGCAGGATATCCGG CACTGCTACAGCATCCAGCACTGCCCCTTGAAAGGATCATGGTCAGAGTGGAGTACCTGGGGGCTGTGC ATGCCCCCCTGTGGACCTAATCCTACCCGTGCCCGCCAGCGCCTCTGCACACCCTTGCTCCCCAAGTAC CCGCCCACCGTTTCCATGGTCGAAGGTCAGGGCGAGAAGAACGTGACCTTCTGGGGGAGACCGCTGCCA CGGTGTGAGGAGCTACAAGGGCAGAAGCTGGTGGTGGAGGAGAAACGACCATGTCTACACGTGCCTGCT TGCAAAGACCCTGAGGAAGAGGAACTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145252 |
| Insert Size | 1410 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145252.1 |
| RefSeq Size | 1669 bp |
| RefSeq ORF | 1410 bp |
| Locus ID | 5199 |
| UniProt ID | P27918 |
| Protein Families | Secreted Protein |
| MW | 51.3 kDa |
| Gene Summary | This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009] Transcript Variant: This variant (2) differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Both variants 1 and 2 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227711 | CFP (Myc-DDK-tagged)-Human complement factor properdin (CFP), transcript variant 2 |
CNY 3,656.00 |
|
| RC227711L3 | Lenti ORF clone of Human complement factor properdin (CFP), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC227711L4 | Lenti ORF clone of Human complement factor properdin (CFP), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG227711 | CFP (tGFP-tagged) - Human complement factor properdin (CFP), transcript variant 2 |
CNY 4,370.00 |
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