GUCY1A1 (NM_001130685) Human Untagged Clone

CAT#: SC325101

GUCY1A3 (untagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 5



  "NM_001130685" in other vectors (4)

CNY 7,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


GUCY1A1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3; GUCA3; GUCSA3; GUCY1A3; MYMY6
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_001130685, the custom clone sequence may differ by one or more nucleotides
ATGCCTCCCTGCTTCCATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTGTTGTAC
TCCGTTCACATGAAAAGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTG
GTGATTCCCACATCGCTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGAT
ATGACAATTCTGCAATTTGGCAATGGCATCAGAAGGCTGATGAACAGGAGAGACTTTCAA
GGAAAGCCTAATTTTGAAGAATACTTTGAAATTCTGACTCCAAAAATCAACCAGACGTTT
AGCGGGATCATGACTATGTTGAATATGCAGTTTGTTGTACGAGTGAGGAGATGGGACAAC
TCTGTGAAAAAATCTTCAAGGGTTATGGACCTCAAAGGCCAAATGATCTACATTGTTGAA
TCCAGTGCAATCTTGTTTTTGGGGTCACCCTGTGTGGACAGATTAGAAGATTTTACAGGA
CGAGGGCTCTACCTCTCAGACATCCCAATTCACAATGCACTGAGGGATGTGGTCTTAATA
GGGGAACAAGCCCGAGCTCAAGATGGCCTGAAGAAGAGGCTGGGGAAGCTGAAGGCTACC
CTTGAGCAAGCCCACCAAGCCCTGGAGGAGGAGAAGAAAAAGACAGTAGACCTTCTGTGC
TCCATATTTCCCTGTGAGGTTGCTCAGCAGCTGTGGCAAGGGCAAGTTGTGCAAGCCAAG
AAGTTCAGTAATGTCACCATGCTCTTCTCAGACATCGTTGGGTTCACTGCCATCTGCTCC
CAGTGCTCACCGCTGCAGGTCATCACCATGCTCAATGCACTGTACACTCGCTTCGACCAG
CAGTGTGGAGAGCTGGATGTCTACAAGGTGGAGACCATTGGCGATGCCTATTGTGTAGCT
GGGGGATTACACAAAGAGAGTGATACTCATGCTGTTCAGATAGCGCTGATGGCCCTGAAG
ATGATGGAGCTCTCTGATGAAGTTATGTCTCCCCATGGAGAACCTATCAAGATGCGAATT
GGACTGCACTCTGGATCAGTTTTTGCTGGCGTCGTTGGAGTTAAAATGCCCCGTTACTGT
CTTTTTGGAAACAATGTCACTCTGGCTAACAAATTTGAGTCCTGCAGTGTACCACGAAAA
ATCAATGTCAGCCCAACAACTTACAGATTACTCAAAGACTGTCCTGGTTTCGTGTTTACC
CCTCGATCAAGGGAGGAACTTCCACCAAACTTCCCTAGTGAAATCCCCGGAATCTGCCAT
TTTCTGGATGCTTACCAACAAGGAACAAACTCAAAACCATGCTTCCAAAAGAAAGATGTG
GAAGATGGCAATGCCAATTTTTTAGGCAAAGCATCAGGAATAGAT
Restriction Sites Please inquire     
ACCN NM_001130685
Insert Size 9149 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001130685.1, NP_001124157.1
RefSeq Size 9149 bp
RefSeq ORF 1368 bp
Locus ID 2982
Protein Families Druggable Genome
Protein Pathways Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
Gene Summary Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (B) is shorter at the N-terminus compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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