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Sphingomyelin Synthase 2 (SGMS2) (NM_001136257) Human Untagged Clone
CAT＃: SC325000
SGMS2 (untagged)-Human sphingomyelin synthase 2 (SGMS2), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDL; SMS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325000 representing NM_001136257.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATATCATAGAGACAGCAAAACTTGAAGAACATTTGGAAAATCAACCCAGTGATCCTACGAACACT TATGCAAGACCCGCTGAACCTGTTGAAGAAGAAAACAAAAATGGCAATGGTAAACCCAAGAGCTTATCC AGTGGGCTGCGAAAAGGCACCAAAAAGTACCCGGACTATATCCAAATTGCTATGCCCACTGAATCAAGG AACAAATTTCCACTAGAGTGGTGGAAAACGGGCATTGCCTTCATATATGCAGTTTTCAACCTCGTCTTG ACAACCGTCATGATCACAGTTGTACATGAGAGGGTCCCTCCCAAGGAGCTTAGCCCTCCACTCCCAGAC AAGTTTTTTGATTACATTGATAGGGTGAAATGGGCATTTTCTGTATCAGAAATAAATGGGATTATATTA GTTGGATTATGGATCACCCAGTGGCTGTTTCTGAGATACAAGTCAATAGTGGGACGCAGATTCTGTTTT ATTATTGGAACTTTATACCTGTATCGCTGCATTACAATGTATGTTACTACTCTACCTGTGCCTGGAATG CATTTCCAGTGTGCTCCAAAGCTCAATGGAGACTCTCAGGCAAAAGTTCAACGGATTCTACGATTGATT TCTGGTGGTGGATTGTCCATAACTGGATCACATATCTTATGTGGAGACTTCCTCTTCAGCGGTCACACG GTTACGCTGACACTGACTTATTTGTTCATCAAAGAATATTCGCCTCGTCACTTCTGGTGGTATCATTTA ATCTGCTGGCTGCTGAGTGCTGCCGGGATCATCTGCATTCTTGTAGCACACGAACACTACACTATCGAT GTGATCATTGCTTATTATATCACAACACGACTGTTTTGGTGGTACCATTCAATGGCCAATGAAAAGAAC TTGAAGGTCTCTTCACAGACTAATTTCTTATCTCGAGCATGGTGGTTCCCCATCTTTTATTTTTTTGAG AAAAATGTACAAGGCTCAATTCCTTGCTGCTTCTCCTGGCCGCTGTCTTGGCCTCCTGGCTGCTTCAAA TCATCATGCAAAAAGTATTCACGGGTTCAGAAGATTGGTGAAGACAATGAGAAATCGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001136257 |
| Insert Size | 1098 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001136257.1 |
| RefSeq Size | 5970 bp |
| RefSeq ORF | 1098 bp |
| Locus ID | 166929 |
| UniProt ID | Q8NHU3 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 42.3 kDa |
| Gene Summary | Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227377 | SGMS2 (Myc-DDK-tagged)-Human sphingomyelin synthase 2 (SGMS2), transcript variant 2 |
CNY 3,656.00 |
|
| RC227377L3 | Lenti ORF clone of Human sphingomyelin synthase 2 (SGMS2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC227377L4 | Lenti ORF clone of Human sphingomyelin synthase 2 (SGMS2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG227377 | SGMS2 (tGFP-tagged) - Human sphingomyelin synthase 2 (SGMS2), transcript variant 2 |
CNY 4,370.00 |
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