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MRPS27 (NM_015084) Human Untagged Clone
CAT＃: SC324057
MRPS27 (untagged)-Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRP-S27; S27mt |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_015084.1
CTCCAAGATGGCTGCCTCCATAGTGCGGCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCT
TCCTCAGCTCTCTCCTGCAGGTAAAAGATACCTGCTTTCTTCAGCCTATGTAGACAGCCA CAAATGGGAAGCAAGAGAAAAAGAACATTACTGTCTTGCTGATCTTGCATCTTTAATGGA TAAAACATTTGAGAGAAAGTTGCCTGTTAGTTCTTTAACAATATCACGGCTTATAGACAA CATTTCCTCTCGGGAAGAGATAGATCATGCAGAGTATTACCTTTACAAGTTTCGACACAG CCCCAACTGCTGGTACCTGAGAAACTGGACTATCCACACCTGGATTAGGCAGTGTCTAAA ATATGATGCACAAGACAAAGCCCTATATACCCTTGTAAATAAGGTTCAATATGGAATTTT TCCAGATAACTTTACATTCAATTTACTGATGGATTCTTTCATAAAGAAAGAAAATTACAA AGATGCTTTATCTGTGGTTTTTGAGGTCATGATGCAAGAAGCCTTTGAAGTGCCTTCCAC CCAACTTCTCTCCCTCTATGTTTTATTTCATTGCCTGGCAAAGAAGACAGACTTCAGTTG GGAAGAGGAGAGGAACTTTGGTGCATCCCTTTTGCTTCCAGGCCTAAAACAAAAGAACTC AGTGGGTTTCAGTTCCCAGTTGTATGGCTATGCACTTCTTGGGAAGGTGGAGTTGCAGCA AGGGCTACGGGCTGTGTACCACAACATGCCTCTGATATGGAAACCAGGCTACCTTGACAG AGCCCTTCAAGTGATGGAGAAAGTGGCTGCCTCCCCAGAAGACATAAAGCTGTGTAGAGA AGCGCTCGATGTGCTGGGTGCAGTGCTGAAGGCTCTGACTTCAGCTGATGGGGCTTCAGA GGAGCAGTCCCAAAATGATGAAGACAACCAGGGGTCAGAAAAACTGGTGGAGCAGTTAGA CATCGAGGAAACAGAGCAGTCCAAGCTTCCTCAATACCTGGAACGATTTAAGGCCTTACA TTCTAAGCTTCAAGCTCTGGGCAAAATTGAGTCAGAAGGTCTTTTAAGTCTGACCACCCA GCTTGTCAAGGAAAAACTCTCCACCTGTGAAGCAGAGGACATCGCCACCTATGAGCAGAA TCTGCAGCAGTGGCATCTAGACCTTGTACAGTTGATCCAGAGAGAACAGCAACAGAGGGA GCAAGCGAAGCAGGAGTACCAGGCTCAGAAAGCAGCAAAGGCATCTGCCTAATAGGGTCC CCCAGGGCCCCACCTGTCTCACAAGAACTTCACTCAACCCCGTGCCAGGACTCAGCAGTG GCCTGGACAACAGCCTCAGCTTCCTCTACCCATCTTCTTTTCTTAAAGCAGGCTATGTGC CCTACATGGCAAGGCACCATGACTGCCCATCGAGATGCCAAGAAGGGCTATGGAACTATG CAGGTGGCTAGTGGTCAGACTGAAGTCACCAGCTGAATACCTTAAGGAGGACTCTTGAGG CTCATAATGGAGTTCCTGGGGCACAGGGATTAGTTATGAGCATTAAAGTTCCTAAGACCC AGTGACAGTACTGGGAGAAACCAAGGCTGAAGAGTCAGGTTGAAGCACAGGCTTTTGTTT TTTGTTGTTTTTGTTTTTTGAGACAGAGTCTCATTCTGTCACCCAGGCTGGAGTGCAGTG GCGCAATCTTGGCTCACTGCAACCTCCGACTCCCAGGTTCAAGCAATTCTCCTGCCTCGG CCTCCTTTAGTAGGTGGGATTACAGGTGCATACCACCACACCTTTCTAATTTTTGCATTT TTAGTAGAGATGGGGTTTCACCATGTTGGTCAGGCTGGTCTCAACCTCCTGACCTCAAGT GATCTGCCCGCCTCAGCCTCCCAAAGTGCTGGGATGATAGGCGTGAGCCACCACACCCGG CCAAGCACAGGCTTTTGAATGGTCTCCCTCTCCCCAGCCCAGGTACATGAGGCCAAGGTG AACTGTGCATCCTGAGACCTTGTGTCCCTGAGTCTCCTTTCTGAGTGCAAGCTGCACTGT GAGCTGGCTGTGGGATACTCACACATTCCCACATTCTCCTTTCTGCCACATCTCGCCCTT CTGGACCTGCACTGGGAGAAATTCAGGACAGGAGTGAACTCAAGGCCATGAACTCACTGG ATTTCCATTTTTAGGCACCCATAGGGATGTTTTTAGGATGTAAGTTTCTGAGGAGAAAGC TAGCTCTAGCATGAAGCTTTTTTGGATTGGCTTCTCCATCGTGTTGGGGTAACATTTTTT TTCCAATTTTTTTTTTCCCAAAACATCGTCTCAGCTCATTTATCAAGTAGGTAAAATGAG CCTTTTGTGTACACACAGAGGCACATGTGCACGCACACACAACTTGTGAACACACATTTC TGTTAAAGAAGTTAGAAAATGAGAGATGGGTTGGGGCTTGAAGTGCATCAGAGGTATGAA TGTTGTAAAACTGTCAGGAGATGTAAAATTCCTTTCTGAAGTGTCTCTTCTGTGAAAGGG TTCAGAGCAGATTTTGCTTACTATGTAGTGTTGCCCTTAAGTACAGTGGTGTAATTTTAT TCAAGATTGTGCTCTTCTATCAAAAGCCTCTGGAAATAAATGTTCCGGGATCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_015084 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015084.1, NP_055899.1 |
| RefSeq Size | 2631 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 23107 |
| UniProt ID | Q92552 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209478 | MRPS27 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| RC209478L1 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209478L2 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC209478L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209478L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG209478 | MRPS27 (tGFP-tagged) - Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein |
CNY 5,256.00 |
|
| SC101669 | MRPS27 (untagged)-Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
