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MID1 (NM_000381) Human Untagged Clone
CAT＃: SC324026
MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1
CNY 4,896.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000381.1
GTCTCGAGCAGAGTGCGTGTAGCAACAGATCAAAGAAAAAGAGGACGAAAACGTGCTCTT
TGCTGCCCGTAGATTTCGCCGGGTTGCTTTTGTCTTGCGGGCTCCTGTCGGGTTCGGTGT TTCCGCTCTGAAGACTGCGACGCGGGCTCCGATGCAGCTCGCTCCCTGCCGGATGGGTCA TGGGATTCTAAACATGAGGCAGATAGCTGATCAGCTTCCTTGGGTTTTGCTGATGACACA AGAGAGCTTTGCCTGAAGATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTG GAGCTCTTTGAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCC CACCGCATCCTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTC CAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAG CGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCC AACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCC GAGAAGGTCCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGT GTCACTTGTGAAGTATCCTACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAG CCCTTTACAGGCCATCGTCTGATTGAGCCAATTCCGGACTCTCACATCCGGGGGCTGATG TGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTGTGTGACCGATGACCAGTTAATC TGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCAGCTTTGAGTGAG CGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGAAC ACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAAT GCATCACGTCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAG CAAAGACGACAGATTATTGGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAA CTGGCTCAGCAGATTGCAAACTGCAAACAGTGCATTGAGCGGTCAGCATCACTCATCTCC CAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCGTTTCCTACAGACTGCTAAGAAT ATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATTCCTGAAATCAAC CTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAGAA TGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCT TCATATGACACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTAC GAGCTCCAGTACACCATATTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCT GATAGCTGGATGATAGTACCCAACATCAAGCAGAACCACTACACGGTGCACGGTCTGCAG AGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAACCAGGCGGGCAGCCGCAGCAGT GAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCCAAATCTGCTCAT CGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCAAG AAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTG TTTATTGATAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCC ATTGGTCTTGCTTACAAATCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCC TGGGCGCTCTGCCGCTGCAACAATAACTGGGTGGTGAGACACAATAGCAAGGAAATCCCC ATTGAGCCTGCCCCCCACCTCCGGCGCGTGGGCATCCTGCTGGACTATGATAACGGCTCT ATCGCCTTTTATGATGCTTTGAACTCCATCCACCTCTACACCTTCGACGTCGCATTTGCG CAGCCTGTTTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGCTC CCTATCCCAGACCATTTGGACTGCACAGAGCAGCTGCCGTGAGCGTCTGGCCACATGGAG CTGCTTTCTGGGGAACAGTAAGGTTCAGGCCACTATTTAGGGGACTGAGAAAGCACAGGC TTCATGAGTGTAATGAAATCTCACCAGAAGTGTCCCGAAATCGGCTCAGATAGGGCTCAA AACAAGAGATTCCTCTCCTTTTACTGTGTCTTGTATTAAGTACGGGCTTTAATAATTTCT TTAATTTTTTTGTATTTAGAGGAAAATCTATAGATTATTTATAAGAGAAACATAATCAGG ATTACAACTTTTAGGAATTACTTGGTTTTGCACATTAAGAGGCCCATAAGTTTATCAGCT ATTTACAACCTTCATTTCATCACAATCTGTGGGCTTACAAAAAAACAAAAACTTTTGTAG TTTTGTATGTTACTCATCTTCTTACCTGATATCCCATGATGATCCCATGGTAGGTCTTCT CACCTCGATGGTGCATAACAGGATGTGTTTGAACCTAGTAGGGGAGGAAACAGGCTTTCT TACTCTGGTTTAATTTGAAGTGTTTTAATTGTGATGTCAAAAAGTTGTATCAGATCAACT AAAATGGAGAGCAAGACAGAGAATGAAAAGAGTTGATTTTGGACCTCGGACCTTGCCGTG GCTAAATCTTTACCTTCTCATAGCTGATGGGATAATGTTGGAAAGAAAGGTTCTGAATCC TTTGGCCACATTTTGCCCTGCTTCTCTCAGGGTTAAGGGTTCTGGAAGAACATTAAGAAT GAGATTGCAATTGAAAATAGTCATTTTGAATCCTATTGATTATTCAAAAATTCAGGCTGA TTTGTCTTTTATCAGAGGTAGGATTCTGTTTTATAGTATAGAATCTACTTTATCCTTCCT TTTAATAGTTCCTTTAGACCTGTGAAATTTCTTCACTACATTTAATAGTTCTCCTATTTC CCGCTCCCCCATATCAATTTTCCTTTTGTCTCCGGGGCTGAGTAAATAAACATGTTCTGT CACAAATAGCAGCACCACTTTGGATTGATTTTGCTCTCCAGGACATCAGCACATGGCCCT GATCAGCACTACCACATCCAAACATAAGTCACTGAAAAACACTTAATATTTATGAGTTGG TAATGACAAGGGACATTGTATAAAGTACTATTTGCTAGATTCATGCCTCAAAAGTTATTA TAAACAGACCTTTATTAAACACATCTTGAAAGATGTAGAAGTCCCTCTATAGTCTAGTAT AGTTTACAATAGAGTTGTAAGACCAAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_000381 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000381.1, NP_000372.1 |
| RefSeq Size | 3452 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 4281 |
| UniProt ID | O15344 |
| Domains | zf-B_box, RING, BBC, SPRY, FN3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1, also known as alpha) is the longest transcript. Variants 1-4 and variant 10 encode the same isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208776 | MID1 (Myc-DDK-tagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1 |
CNY 4,888.00 |
|
| RC208776L1 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, Myc-DDK-tagged |
CNY 7,288.00 |
|
| RC208776L2 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208776L3 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208776L4 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208776 | MID1 (tGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1 |
CNY 6,488.00 |
|
| SC109438 | MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1 |
CNY 4,896.00 |
