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Epac1 (RAPGEF3) (NM_006105) Human Untagged Clone
CAT＃: SC323918
RAPGEF3 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2
CNY 6,464.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_006105.3
CTGCTGCCCTGGCAGCCAGTCCAGCTAGGCACTGGCCCAGTTCACTGTACCTGGACTTAC
CCACTGGCACCTTCCGGATCCCCTTATCAAAGCTGATGGGGGTCGCTGGGGACCCCCCAG CCTTTTGCTAGAGCCTGCACGGCTGTGCGAGCTTGAAAAGAAACATGAAGGTGGGCTGGC CAGGTGAGAGCTGCTGGCAGGTGGGCCTGCCTGTGGAGGATAGCCCAGCTCTGGGAGCAC CGCGGGTGGGAGCCCTCCCTGACGTGGTGCCGGAGGGGACACTACTCAACATGGTGTTGA GAAGGATGCACCGGCCCCGAAGCTGCTCCTACCAGCTGCTGCTGGAGCACCAGCGTCCGA GCTGCATCCAGGGGCTGCGCTGGACACCACTCACCAACAGCGAGGAGTCCCTGGATTTCA GCGAGAGCCTGGAGCAGGCCTCCACAGAGCGGGTGCTCAGGGCTGGGAGGCAGCTGCATC GGCATCTGCTGGCCACCTGCCCAAACCTCATCCGAGACCGGAAGTACCACCTTAGGCTCT ATCGGCAGTGCTGCTCTGGCCGGGAGCTGGTGGATGGGATCTTGGCCCTGGGACTTGGGG TCCATTCCCGGAGCCAAGTTGTGGGAATCTGCCAGGTGCTGCTGGATGAAGGTGCCCTCT GCCATGTGAAACACGACTGGGCCTTCCAGGACCGAGATGCCCAATTCTACCGGTTCCCCG GGCCCGAGCCCGAGCCCGTGGGAACTCATGAGATGGAGGAGGAGTTGGCCGAAGCTGTGG CCCTGCTCTCCCAGCGGGGGCCTGACGCCCTGCTCACTGTGGCACTTCGAAAGCCCCCAG GTCAGCGCACGGATGAAGAGCTGGACCTCATCTTTGAGGAGCTGCTGCACATCAAGGCTG TGGCCCACCTCTCCAACTCGGTGAAGCGAGAATTAGCGGCTGTTCTGCTCTTTGAACCAC ACAGCAAGGCAGGGACCGTGTTGTTCAGCCAGGGGGACAAGGGCACTTCGTGGTACATTA TCTGGAAGGGATCTGTCAACGTGGTGACCCATGGCAAGGGGCTGGTGACCACCCTGCATG AGGGAGATGATTTTGGACAGCTGGCTCTGGTGAATGATGCACCCCGGGCAGCCACCATCA TCCTGCGAGAAGACAACTGTCATTTCCTGCGTGTGGACAAGCAGGACTTCAACCGTATCA TCAAGGATGTGGAGGCAAAGACCATGCGGCTGGAAGAACATGGCAAAGTGGTGCTGGTGC TGGAGAGAGCCTCTCAGGGCGCCGGCCCTTCCCGACCCCCAACCCCAGGCAGGAACCGGT ATACAGTGATGTCTGGCACCCCAGAGAAGATCCTAGAGCTTCTGTTGGAGGCCATGGGAC CAGATTCCAGTGCTCATGACCCAACAGAGACATTCCTCAGCGACTTCCTCCTGACCCACA GGGTCTTCATGCCCAGCGCCCAACTCTGCGCTGCCCTTCTGCACCACTTCCATGTGGAGC CTGCGGGTGGCAGCGAGCAGGAGCGCAGCACCTACGTCTGCAACAAGAGGCAGCAGATCT TGCGGCTGGTCAGCCAGTGGGTGGCCCTGTATGGCTCCATGCTCCACACTGACCCTGTGG CCACCAGCTTCCTCCAGAAACTCTCAGACCTGGTGGGCAGGGACACCCGACTCAGCAACC TGCTGAGGGAGCAGTGGCCAGAGAGGCGGCGATGCCACAGGTTGGAGAATGGCTGTGGGA ATGCATCTCCTCAGATGAAGGCCCGGAACTTGCCTGTTTGGCTCCCCAACCAGGACGAGC CCCTTCCTGGCAGCAGCTGTGCCATCCAAGTTGGGGATAAAGTCCCCTATGACATCTGCC GGCCAGACCACTCAGTGTTGACCCTGCAGCTGCCTGTGACAGCCTCCGTGAGAGAGGTGA TGGCAGCGTTGGCCCAGGAGGATGGCTGGACCAAGGGGCAGGTGCTGGTGAAGGTCAATT CTGCAGGTGATGCCATTGGCCTGCAGCCAGATGCCCGTGGTGTGGCCACATCTCTGGGGC TCAATGAGCGTCTCTTTGTTGTCAACCCACAGGAAGTGCATGAGCTGATCCCACACCCTG ACCAGCTGGGGCCCACTGTGGGCTCTGCTGAGGGGCTGGACCTGGTGAGTGCCAAGGACC TGGCAGGCCAGCTGACGGACCACGACTGGAGCCTCTTCAACAGTATCCACCAGGTGGAGC TGATCCACTATGTGCTGGGCCCCCAGCATCTGCGGGATGTCACCACCGCCAACCTGGAGC GCTTCATGCGCCGCTTCAATGAGCTGCAGTACTGGGTGGCCACCGAGCTGTGTCTCTGCC CCGTGCCCGGCCCCCGGGCCCAGCTGCTCAGGAAGTTCATTAAGCTGGCGGCCCACCTCA AGGAGCAGAAGAATCTCAATTCCTTCTTTGCCGTCATGTTTGGCCTCAGCAACTCGGCCA TCAGCCGCCTAGCCCACACCTGGGAGCGGCTGCCTCACAAAGTCCGGAAGCTGTACTCCG CCCTCGAGAGGCTGCTGGATCCCTCATGGAACCACCGGGTATACCGACTGGCCCTCGCCA AGCTCTCCCCTCCTGTCATCCCCTTCATGCCCCTTCTTCTCAAAGACATGACCTTCATTC ATGAGGGAAACCACACACTAGTGGAGAATCTCATCAACTTTGAGAAGATGAGAATGATGG CCAGAGCCGCGCGGATGCTGCACCACTGCCGAAGCCACAACCCTGTGCCTCTCTCACCAC TCAGAAGCCGAGTTTCCCACCTCCACGAGGACAGCCAGGTGGCGAGGATTTCCACATGCT CGGAGCAGTCCCTGAGCACCCGGAGTCCAGCCAGCACCTGGGCTTATGTCCAGCAGCTGA AGGTCATTGACAACCAGCGGGAACTCTCCCGCCTCTCCCGAGAGCTGGAGCCATGAGGAG GGGCTGGGACTGGAGCTGGAGCAGGCACTTGCAGCCGGGAAAGCCAGGGTGTGCCGGGCC AAGATACTCACAGGCTGGCCACAGCTGGGCAAGGCTCTCCGTGGAGTGGACTCGAGTCCC TGGAGCAGGCAGTGTGGAGGCAGCCATCCCCTGTGATGACTGGCAGCTAAGGAGGACCTC GGAGTGGACCCGAGCCAGGAATAACGAATGACCCAAGGCCAAGGAAGGGAGGACAGAGAG GCCCCAGGAGTGGGTGGAGAGTGGAGTGTGCTGGGACGTTGTGTGCAATAGAGAGGTCTC CACACCAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_006105 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006105.3, NP_006096.2 |
| RefSeq Size | 3327 bp |
| RefSeq ORF | 2646 bp |
| Locus ID | 10411 |
| UniProt ID | O95398 |
| Domains | DEP, cNMP, RasGEFN, RasGEF |
| Protein Pathways | Leukocyte transendothelial migration, Long-term potentiation |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate 5' exon and differs in the 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream AUG and an isoform (b) with a shorter N-terminus compared to isoform a. Variants 2 and 3 encode the same isoform. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204518 | RAPGEF3 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2 |
CNY 6,768.00 |
|
| RC204518L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204518L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204518 | RAPGEF3 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2 |
CNY 8,368.00 |
|
| SC116327 | RAPGEF3 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2 |
CNY 6,464.00 |
