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Ephexin 1 (NGEF) (NM_019850) Human Untagged Clone
CAT＃: SC323833
NGEF (untagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1
CNY 5,208.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARHGEF27; EPHEXIN |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_019850.1
CAGGACGAAGTTTTTGGCTGTGGGCTCTCTGGGACAGAGTGGAGCGCCTGTGGATTTAGC
CGGGGACACACCATCCTGCTCGCAGGTGCCTGGACAGGACAGGGGACGAGGGAGGAACAC TCTCTGAGGAAGCCCAACTCCTATGAGCTCCTCTCTGGCAGCACTCAAACCAGTGAAGCT TGGGAAAGTCATTGACCTCCAGTCGTTCTGCTGAGAAACATCTGGCTCTATTTCCATGGA GACCAGGGAATCTGAAGATTTGGAAAAGACCCGGAGGAAATCAGCAAGTGATCAATGGAA CACTGATAATGAACCAGCCAAGGTGAAACCTGAGTTACTCCCAGAAAAAGAGGAGACTTC TCAAGCTGACCAGGATATCCAAGACAAAGAGCCTCATTGCCACATCCCAATTAAGAGAAA TTCCATCTTCAATCGCTCCATAAGACGCAAAAGCAAAGCCAAGGCCAGAGACAACCCCGA ACGGAACGCCAGCTGCCTGGCAGATTCACAGGACAATGGAAAATCTGTAAATGAGCCCCT GACCTTGAATATCCCCTGGAGCAGAACGCCTCCTTGCAGAACAGCAATGCAGACAGACCC AGGAGCCCAGGAAATGAGTGAGTCGTCCTCCACCCCGGGAAATGGGGCCACGCCCGAGGA GTGGCCGGCCCTGGCCGACAGCCCCACCACGCTCACCGAGGCCCTGCGGATGATCCACCC CATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAGGAATA CCGAGATAAATCGACTCTCCAAGAAATCGAAACCAGGAGGCAACAGGATGCAGAAATAGA AGACAATACCAATGGGTCCCCGGCCAGTGAGGACACCCCGGAGGAGGAAGAAGAAGAGGA GGAGGAGGAGGAGCCGGCCAGCCCACCAGAGAGGAAGACTCTGCCCCAGATCTGCCTGCT CAGTAACCCCCACTCAAGGTTCAACCTCTGGCAGGATCTTCCCGAGATCCGGAGCAGCGG GGTGCTTGAGATCCTACAGCCTGAGGAGATTAAGCTGCAGGAGGCCATGTTCGAGCTGGT CACTTCCGAGGCGTCCTACTACAAGAGTCTGAACCTGCTCGTGTCCCACTTCATGGAGAA CGAGCGGATAAGGAAGATCCTGCACCCGTCCGAGGCGCACATCCTCTTCTCCAACGTCCT GGACGTGCTGGCTGTCAGTGAGCGGTTCCTCCTGGAGCTGGAGCACCGGATGGAGGAGAA CATCGTCATCTCTGACGTGTGTGACATCGTGTACCGTTATGCGGCTGACCACTTCTCTGT CTACATCACCTACGTCAGCAATCAGACCTACCAGGAGCGGACCTATAAGCAGCTGCTCCA GGAGAAGGCAGCTTTCCGGGAGCTGATCGCGCAGCTAGAGCTCGACCCCAAGTGCAGGGG GCTGCCCTTCTCCTCCTTCCTCATCCTGCCTTTCCAGAGGATCACACGCCTCAAGCTGTT GGTCCAGAACATCCTGAAGAGGGTAGAAGAGAGGTCTGAGCGGGAGTGCACTGCTTTGGA TGCTCACAAGGAGCTGGAAATGGTGGTGAAGGCATGCAACGAGGGCGTCAGGAAAATGAG CCGCACGGAACAGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTGCC CATCATCTCCCACTCCCGCTGGCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCAGGCCC CAAGACCTCCCGGACCCTGAGGACCAAGAAGCTCTTCCACGAAATTTACCTCTTCCTGTT CAACGACCTGCTGGTGATCTGCCGGCAGATTCCAGGAGACAAGTACCAGGTATTTGACTC AGCTCCGCGGGGACTGCTGCGTGTGGAGGAGCTGGAGGACCAGGGCCAGACGCTGGCCAA CGTGTTCATCCTGCGGCTGCTGGAGAACGCAGATGACCGGGAGGCCACCTACATGCTAAA GGCGTCCTCTCAGAGTGAGATGAAGCGTTGGATGACCTCACTGGCCCCCAACAGGAGGAC CAAGTTTGTTTCGTTCACATCCCGGCTGCTGGACTGCCCCCAGGTCCAGTGCGTGCACCC ATACGTGGCTCAGCAGCCAGACGAGCTGACGCTGGAGCTCGCCGACATCCTCAACATCCT GGACAAGACTGACGACGGGTGGATCTTTGGCGAGCGTCTGCACGACCAGGAGAGAGGCTG GTTCCCCAGCTCCATGACTGAGGAGATCTTGAATCCCAAGATCCGGTCCCAGAACCTCAA GGAATGTTTCCGTGTCCACAAGATGGATGACCCTCAGCGCAGCCAGAACAAGGACCGCAG GAAGCTGGGCAGCCGGAATCGGCAATGACCCCCACCCAGGGGGCCAGCGGGAGCAGGGCC TGCATGAGACCCCGACAGAAGGTGGGGGGGGGGCCTCTGGGAAGCACAGGCCAGCACCTC CCCAGGTGGCAGGATCTGGCTTGGGGTGCCCGGCCCTCATCCCTGCCCACGCAGTGAGTG CTCATGTGTCTTGGCCCCTTGCTTGCAAACTGGATAAAGGGTGCCCAAGCCTCTCCTGAT GCATTTGTAAACAAGAAGGTTTCAGCAGTATTACACCACCTCCCTCATGCCTCCGAGGGG GTGGAAGGGGGTGGGCACACTCCAGGGCCCCCCATGCCCCTGGCCCCCAGGGACTGGAAG AGGCTCCCAACCCAGAGTGTCCCTGTGGGAGGCAGGCAGAAGGTGACAATTGACACGATT TCCTGCACGCGTCCTCCTCTACCTTGGAAGCAGTTAGAATCTACCAGGCACAGATGAGGC CGCCCTTGCCTGACGGAGCTTGATGAGCAGCCCTTGGTCTCCGGTTCCAGGACTGAGAGC CCAGCTGCCTCTGCCCACCCTTCCCCAGGCCTCTGCCAGCCTCTGGCTGCACGGTCAGGC CCTGCCCCATGGCAGGCCTGCCAGAGCTTGGCTGGGGACCCCTCCCGCCTCTGGCTCCCT GATGGGCTGGATGTAACTTGTGTCTTCTAGCCGCTTAAGGAGCCCAGGTGTTTTAAGGAA TGAATTGGTCACTGCATCTTGTATCGATTATGGTTCTGAGAAAAGCAAATATCACTTTTG GCTGCATTAAAAGAAGCATCATATATAAAATAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_019850 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019850.1, NP_062824.1 |
| RefSeq Size | 3177 bp |
| RefSeq ORF | 2133 bp |
| Locus ID | 25791 |
| UniProt ID | Q8N5V2 |
| Protein Pathways | Axon guidance |
| Gene Summary | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206775 | NGEF (Myc-DDK-tagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1 |
CNY 5,200.00 |
|
| RC206775L1 | Lenti ORF clone of Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC206775L2 | Lenti ORF clone of Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC206775L3 | Lenti ORF clone of Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC206775L4 | Lenti ORF clone of Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG206775 | NGEF (tGFP-tagged) - Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1 |
CNY 6,800.00 |
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