CDC2L5 (CDK13) (NM_003718) Human Untagged Clone

CAT#: SC323704

CDK13 (untagged)-Kinase deficient mutant (K120M) of Human cell division cycle 2-like 5 (cholinesterase-related cell division controller) (CDC2L5), transcript variant 1



  "NM_003718" in other vectors (7)

CNY 29,070.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


CDK13 Antibody - N-terminal region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003718, the custom clone sequence may differ by one or more nucleotides


ATGCCGAGCAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCGGAGAAGAAGTTGGAGG
AACGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA
GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCTCCCGGCACGGCCGCCGCCGCA
GCCGCCGCCGCCGCGGCCTCCTCCTCTTGCTTCAGCCCGGGCCCCCCTCTGGAGGTCAAGCGGCTGGCGA
GAGGCAAGAGGCGCGCAGGAGGGCGGCAGAAGCGGCGTCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA
GCGTCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCTAGTAGCGGCGGGGGTGTG
ACCCCGCTGGTGGAATACGAGGATGTGAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGGGCCAGCG
CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAGCGGCGGGAGTCCGGCCTCCTCCTCCGGCAC
CCAGCGGCGCGGGGAGGGGTCGGAGCGCAGGCCCCGCCGGGACCGCCGCAGCAGCAGTGGCCGCAGCAAG
GAGCGCCACCGCGAGCACCGGCGGCGGGATGGGCAGCGCGGTGGCAGCGAGGCCTCCAAGTCCCGCAGCC
GCCACAGCCACAGCGGCGAGGAACGGGCCGAGGTCGCCAAGAGCGGCAGCAGCAGCAGCAGCGGCGGCCG
CCGGAAAAGCGCTTCGGCCACATCCAGCAGCAGTAGCAGCCGCAAGGACCGGGACTCGAAGGCCCACCGC
AGCCGGACTAAGTCGTCCAAGGAGCCGCCTTCGGCCTACAAGGAACCGCCCAAGGCCTACCGGGAGGACA
AGACCGAGCCTAAGGCCTACAGGCGGCGGCGGTCCCTCAGCCCACTGGGAGGCCGGGACGACAGCCCGGT
GTCCCACAGGGCCTCTCAGAGCCTGAGGAGCCGCAAGTCCCCCAGCCCGGCAGGAGGTGGCAGCAGCCCC
TATTCTCGGCGGCTGCCGCGCTCCCCGAGCCCCTACAGTCGCCGCCGCTCCCCCAGCTACAGCCGCCACA
GCTCCTACGAGCGGGGCGGCGACGTGTCCCCTAGTCCCTACAGCAGCAGCAGCTGGCGCCGCTCTCGCAG
TCCCTACAGCCCTGTGCTCAGACGGTCTGGAAAATCCCGAAGCAGAAGCCCGTATTCATCTAGGCATTCA
AGATCTCGTAGCAGGCACAGATTGTCTAGATCCAGAAGTCGTCATTCTAGTATTTCTCCTAGCACACTAA
CTCTGAAGAGTAGCCTGGCAGCTGAATTGAACAAGAATAAAAAAGCACGAGCAGCAGAGGCAGCAAGAGC
CGCAGAAGCAGCGAAAGCTGCAGAAGCAACTAAGGCTGCTGAGGCTGCTGCCAAGGCTGCAAAAGCTTCA
AACACTTCTACACCTACCAAGGGGAACACGGAAACTAGTGCCAGTGCATCACAAACAAACCATGTGAAGG
ATGTGAAGAAAATTAAAATTGAACATGCACCTTCTCCCTCAAGTGGTGGAACTTTAAAAAATGACAAAGC
AAAAACAAAGCCACCTCTTCAGGTAACGAAGGTGGAAAATAATTTGATTGTAGATAAAGCCACCAAGAAA
GCAGTCATAGTTGGAAAGGAGAGTAAATCTGCTGCTACAAAGGAGGAATCAGTATCTCTTAAAGAGAAAA
CCAAACCACTTACACCAAGCATAGGAGCCAAGGAGAAGGAGCAACATGTAGCTTTAGTCACCTCTACATT
ACCACCGTTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGAAGCTGATAGCTTACGAGGAAATATTTCA
GTAAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTTCTTGCTGATTTACCGCTGCCCCCTG
AGCTACCAGGAGGAGATGATCTTTCAAAGAGTCCAGAGGAAAAGAAAACAGCAACACAGTTACATAGTAA
AAGGAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAACCAAAGAAAAAGATATTGACTGGGGAAAACGC
TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTACGGACAAGTTTACAAAGCCAGGG
ATAAAGACACTGGAGAAATGGTAGCCTTAAAAAAAGTACGTCTGGATAATGAAAAGGAAGGCTTTCCAAT
TACAGCAATTCGAGAAATTAAAATTCTCCGGCAGCTTACCCATCAGAGTATTATCAATATGAAGGAAATA
GTGACTGATAAAGAAGATGCTTTGGATTTCAAGAAGGACAAAGGTGCATTTTATCTGGTGTTTGAATATA
TGGACCATGATCTGATGGGACTACTGGAATCAGGCTTGGTTCATTTTAATGAAAATCACATAAAGTCATT
TATGAGACAGCTCATGGAGGGTCTGGATTATTGTCATAAGAAGAACTTTTTGCATAGAGATATTAAATGT
TCCAATATCCTTCTAAATAATAGAGGGCAGATAAAACTTGCAGACTTTGGACTTGCTCGATTGTATAGCT
CAGAAGAAAGTCGGCCGTATACTAACAAGGTAATTACTTTATGGTACCGTCCACCTGAACTGCTACTGGG
AGAAGAACGATACACACCAGCCATTGATGTATGGAGCTGTGGCTGTATCCTTGGCGAACTCTTCACTAAA
AAACCTATATTTCAAGCAAATCAGGAACTTGCACAACTAGAATTAATAAGCCGAATATGTGGGAGTCCAT
GTCCTGCAGTGTGGCCTGATGTAATCAAACTACCATATTTCAACACCATGAAACCAAAGAAGCAATATCG
TCGAAAGTTAAGAGAAGAATTTGTTTTTATTCCTGCAGCTGCGCTAGACTTATTTGATTACATGCTTGCC
TTGGATCCTAGTAAGCGCTGCACTGCTGAACAGGCTCTTCAGTGCGAGTTCCTCCGAGATGTGGAACCCT
CAAAAATGCCTCCACCAGATCTCCCTTTATGGCAAGATTGTCATGAGTTATGGAGTAAAAAGCGAAGAAG
ACAGAAGCAGATGGGCATGACTGATGATGTTTCCACAATTAAAGCCCCCAGGAAGGACTTGTCTCTGGGC
TTGGATGACAGCAGAACCAACACACCCCAGGGTGTGCTGCCATCTTCACAGCTGAAATCTCAGGGCAGCT
CAAATGTGGCACCTGTAAAAACAGGCCCTGGACAGCACTTAAACCACAGTGAATTGGCAATTCTACTAAA
CCTACTACAATCTAAAACAAGTGTTAATATGGCTGATTTTGTCCAAGTGTTGAACATTAAGGTAAACTCT
GAGACTCAACAGCAGCTAAATAAAATAAACCTTCCTGCTGGAATTTTGGCAACAGGTGAAAAACAGACAG
ATCCATCAACACCACAACAGGAGTCTTCGAAACCGTTGGGAGGAATTCAGCCTTCTTCTCAGACCATCCA
GCCTAAAGTGGAGACTGATGCTGCCCAGGCGGCTGTGCAGAGTGCATTTGCAGTTCTGTTGACTCAGTTA
ATAAAGGCTCAGCAGTCAAAGCAGAAAGATGTGCTACTAGAAGAGAGGGAAAATGGATCGGGACATGAAG
CGTCATTACAACTCAGGCCACCTCCAGAACCTAGCACTCCGGTGTCGGGACAAGATGACCTCATCCAGCA
TCAAGATATGAGGATCTTGGAGCTAACGCCAGAACCAGACCGGCCTCGAATTCTGCCTCCTGACCAACGA
CCTCCCGAGCCTCCTGAACCACCACCAGTCACTGAGGAAGATCTAGATTATCGGACAGAAAACCAGCATG
TACCCACCACCAGTTCTTCATTAACTGACCCTCATGCCGGAGTGAAGGCAGCCCTGTTACAGCTGCTTGC
TCAGCATCAGCCCCAGGATGACCCCAAAAGAGAAGGTGGGATTGATTATCAAGCAGGAGACACTTACGTG
TCCACTTCAGACTACAAGGACAACTTTGGATCCTCTTCTTTCTCTTCTGCTCCTTATGTTAGCAATGATG
GTCTAGGAAGCAGTTCTGCTCCACCACTAGAACGACGTAGTTTCATTGGAAATTCAGATATTCAGTCTTT
GGATAACTACAGTACTGCTTCATCTCATTCTGGTGGTCCACCTCAGCCTTCTGCCTTTTCTGAGTCATTT
CCCAGTTCAGTAGCTGGATATGGAGACATTTACCTCAATGCTGGTCCCATGTTGTTTAGTGGAGACAAGG
ACCATAGATTTGAATATAGCCATGGTCCTATTGCAGTCCTGGCAAACAGCAGTGACCCTTCCACGGGGCC
AGAGAGTACTCATCCTTTGCCAGCAAAGATGCACAACTATAACTATGGTGGTAACTTACAGGAAAATCCG
AGTGGCCCCAGCCTCATGCATGGACAGACCTGGACTTCTCCTGCCCAAGGACCTGGATATTCACAAGGAT
ACAGGGGACATATTAGCACATCAACTGGCAGAGGCAGAGGCAGAGGGTTACCATACTGA


>OriGene 5' read for NM_003718 unedited
ACCGCCCGTTCGAGCAAAGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCATTTAGGT
GACACTATAGAATACAAGCTACTTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGTGGCGCTTT
TCCCGGCCGGCTCTGGTGCTCGGTGTCCCTCCGCCGCCGCTCCCGTTTCCGGCGGGGGAGATGGCCAGGA
TCTGACCCGGGAGGAGGCCGCACCCGCGCCGCGCTCTGCGGCTGGCTCTAGGCGATGCCGAGCAGCTCGG
ACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCGGAGAAGAAGTTGGAGGAACGCCGCAAGCGGAG
GCGATTCCTGTCCCCTCGGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCAGCTCCTGCGGGCGCCG
CCGCCCCCGCCGCCTCTGCTCTTCGTGGCTGCTGCCGGCACGGCCGCCCGCCACAGCCACCGCTGCCACC
GTTCAAGAATCGTAATTCTGCCCCCAGAGCCCTCAAATTATAAACGAACATGGACGGCCGTCAGTAAAGC
TGATTTCGAACTACCATGCCTTGGACGGAGTGGGAGGAGCAGTTTCTCATAAGTTTAAACATGGCTCCAT
CATATTAATGCGGACATTTTACAAGGATGTTAACTCCTCTCACCCGTATAGTTGAACAAGAAATAGCCCA
TTAGCAGAATTACGTCAGATCATACTACGAGACGCCCGCCCGAA
>SC323704 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GTARCTTTAGTCACTCTACTTACCACCGTTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGAAGCTGAT
AGCTTACGAGGAAATATTTCAGTAAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTTCTTG
CTGATTTACCGCTGCCCCCTGAGCTACCAGGAGGAGATGATCTTTCAAAGAGTCCAGAGGAAAAGAAAAC
AGCAACACAGTTACATAGTAAAAGGAGGCCTAAAATATGTGGGCC
Restriction Sites Please inquire     
ACCN NM_003718
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003718.2, NP_003709.2
RefSeq Size 5246 bp
RefSeq ORF 4539 bp
Locus ID 8621
UniProt ID Q14004
Protein Families Druggable Genome, Protein Kinase
Gene Summary The protein encoded by this gene is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. The exact function of this protein has not yet been determined, but it may play a role in mRNA processing and may be involved in regulation of hematopoiesis. Alternatively spliced transcript variants have been described.[provided by RefSeq, Dec 2009]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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