CDC2L5 (CDK13) (NM_003718) Human Untagged Clone
CAT#: SC323704
CDK13 (untagged)-Kinase deficient mutant (K120M) of Human cell division cycle 2-like 5 (cholinesterase-related cell division controller) (CDC2L5), transcript variant 1
CNY 29,070.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_003718, the custom clone sequence may differ by one or more nucleotides
ATGCCGAGCAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCGGAGAAGAAGTTGGAGG AACGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCTCCCGGCACGGCCGCCGCCGCA GCCGCCGCCGCCGCGGCCTCCTCCTCTTGCTTCAGCCCGGGCCCCCCTCTGGAGGTCAAGCGGCTGGCGA GAGGCAAGAGGCGCGCAGGAGGGCGGCAGAAGCGGCGTCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA GCGTCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCTAGTAGCGGCGGGGGTGTG ACCCCGCTGGTGGAATACGAGGATGTGAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGGGCCAGCG CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAGCGGCGGGAGTCCGGCCTCCTCCTCCGGCAC CCAGCGGCGCGGGGAGGGGTCGGAGCGCAGGCCCCGCCGGGACCGCCGCAGCAGCAGTGGCCGCAGCAAG GAGCGCCACCGCGAGCACCGGCGGCGGGATGGGCAGCGCGGTGGCAGCGAGGCCTCCAAGTCCCGCAGCC GCCACAGCCACAGCGGCGAGGAACGGGCCGAGGTCGCCAAGAGCGGCAGCAGCAGCAGCAGCGGCGGCCG CCGGAAAAGCGCTTCGGCCACATCCAGCAGCAGTAGCAGCCGCAAGGACCGGGACTCGAAGGCCCACCGC AGCCGGACTAAGTCGTCCAAGGAGCCGCCTTCGGCCTACAAGGAACCGCCCAAGGCCTACCGGGAGGACA AGACCGAGCCTAAGGCCTACAGGCGGCGGCGGTCCCTCAGCCCACTGGGAGGCCGGGACGACAGCCCGGT GTCCCACAGGGCCTCTCAGAGCCTGAGGAGCCGCAAGTCCCCCAGCCCGGCAGGAGGTGGCAGCAGCCCC TATTCTCGGCGGCTGCCGCGCTCCCCGAGCCCCTACAGTCGCCGCCGCTCCCCCAGCTACAGCCGCCACA GCTCCTACGAGCGGGGCGGCGACGTGTCCCCTAGTCCCTACAGCAGCAGCAGCTGGCGCCGCTCTCGCAG TCCCTACAGCCCTGTGCTCAGACGGTCTGGAAAATCCCGAAGCAGAAGCCCGTATTCATCTAGGCATTCA AGATCTCGTAGCAGGCACAGATTGTCTAGATCCAGAAGTCGTCATTCTAGTATTTCTCCTAGCACACTAA CTCTGAAGAGTAGCCTGGCAGCTGAATTGAACAAGAATAAAAAAGCACGAGCAGCAGAGGCAGCAAGAGC CGCAGAAGCAGCGAAAGCTGCAGAAGCAACTAAGGCTGCTGAGGCTGCTGCCAAGGCTGCAAAAGCTTCA AACACTTCTACACCTACCAAGGGGAACACGGAAACTAGTGCCAGTGCATCACAAACAAACCATGTGAAGG ATGTGAAGAAAATTAAAATTGAACATGCACCTTCTCCCTCAAGTGGTGGAACTTTAAAAAATGACAAAGC AAAAACAAAGCCACCTCTTCAGGTAACGAAGGTGGAAAATAATTTGATTGTAGATAAAGCCACCAAGAAA GCAGTCATAGTTGGAAAGGAGAGTAAATCTGCTGCTACAAAGGAGGAATCAGTATCTCTTAAAGAGAAAA CCAAACCACTTACACCAAGCATAGGAGCCAAGGAGAAGGAGCAACATGTAGCTTTAGTCACCTCTACATT ACCACCGTTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGAAGCTGATAGCTTACGAGGAAATATTTCA GTAAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTTCTTGCTGATTTACCGCTGCCCCCTG AGCTACCAGGAGGAGATGATCTTTCAAAGAGTCCAGAGGAAAAGAAAACAGCAACACAGTTACATAGTAA AAGGAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAACCAAAGAAAAAGATATTGACTGGGGAAAACGC TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTACGGACAAGTTTACAAAGCCAGGG ATAAAGACACTGGAGAAATGGTAGCCTTAAAAAAAGTACGTCTGGATAATGAAAAGGAAGGCTTTCCAAT TACAGCAATTCGAGAAATTAAAATTCTCCGGCAGCTTACCCATCAGAGTATTATCAATATGAAGGAAATA GTGACTGATAAAGAAGATGCTTTGGATTTCAAGAAGGACAAAGGTGCATTTTATCTGGTGTTTGAATATA TGGACCATGATCTGATGGGACTACTGGAATCAGGCTTGGTTCATTTTAATGAAAATCACATAAAGTCATT TATGAGACAGCTCATGGAGGGTCTGGATTATTGTCATAAGAAGAACTTTTTGCATAGAGATATTAAATGT TCCAATATCCTTCTAAATAATAGAGGGCAGATAAAACTTGCAGACTTTGGACTTGCTCGATTGTATAGCT CAGAAGAAAGTCGGCCGTATACTAACAAGGTAATTACTTTATGGTACCGTCCACCTGAACTGCTACTGGG AGAAGAACGATACACACCAGCCATTGATGTATGGAGCTGTGGCTGTATCCTTGGCGAACTCTTCACTAAA AAACCTATATTTCAAGCAAATCAGGAACTTGCACAACTAGAATTAATAAGCCGAATATGTGGGAGTCCAT GTCCTGCAGTGTGGCCTGATGTAATCAAACTACCATATTTCAACACCATGAAACCAAAGAAGCAATATCG TCGAAAGTTAAGAGAAGAATTTGTTTTTATTCCTGCAGCTGCGCTAGACTTATTTGATTACATGCTTGCC TTGGATCCTAGTAAGCGCTGCACTGCTGAACAGGCTCTTCAGTGCGAGTTCCTCCGAGATGTGGAACCCT CAAAAATGCCTCCACCAGATCTCCCTTTATGGCAAGATTGTCATGAGTTATGGAGTAAAAAGCGAAGAAG ACAGAAGCAGATGGGCATGACTGATGATGTTTCCACAATTAAAGCCCCCAGGAAGGACTTGTCTCTGGGC TTGGATGACAGCAGAACCAACACACCCCAGGGTGTGCTGCCATCTTCACAGCTGAAATCTCAGGGCAGCT CAAATGTGGCACCTGTAAAAACAGGCCCTGGACAGCACTTAAACCACAGTGAATTGGCAATTCTACTAAA CCTACTACAATCTAAAACAAGTGTTAATATGGCTGATTTTGTCCAAGTGTTGAACATTAAGGTAAACTCT GAGACTCAACAGCAGCTAAATAAAATAAACCTTCCTGCTGGAATTTTGGCAACAGGTGAAAAACAGACAG ATCCATCAACACCACAACAGGAGTCTTCGAAACCGTTGGGAGGAATTCAGCCTTCTTCTCAGACCATCCA GCCTAAAGTGGAGACTGATGCTGCCCAGGCGGCTGTGCAGAGTGCATTTGCAGTTCTGTTGACTCAGTTA ATAAAGGCTCAGCAGTCAAAGCAGAAAGATGTGCTACTAGAAGAGAGGGAAAATGGATCGGGACATGAAG CGTCATTACAACTCAGGCCACCTCCAGAACCTAGCACTCCGGTGTCGGGACAAGATGACCTCATCCAGCA TCAAGATATGAGGATCTTGGAGCTAACGCCAGAACCAGACCGGCCTCGAATTCTGCCTCCTGACCAACGA CCTCCCGAGCCTCCTGAACCACCACCAGTCACTGAGGAAGATCTAGATTATCGGACAGAAAACCAGCATG TACCCACCACCAGTTCTTCATTAACTGACCCTCATGCCGGAGTGAAGGCAGCCCTGTTACAGCTGCTTGC TCAGCATCAGCCCCAGGATGACCCCAAAAGAGAAGGTGGGATTGATTATCAAGCAGGAGACACTTACGTG TCCACTTCAGACTACAAGGACAACTTTGGATCCTCTTCTTTCTCTTCTGCTCCTTATGTTAGCAATGATG GTCTAGGAAGCAGTTCTGCTCCACCACTAGAACGACGTAGTTTCATTGGAAATTCAGATATTCAGTCTTT GGATAACTACAGTACTGCTTCATCTCATTCTGGTGGTCCACCTCAGCCTTCTGCCTTTTCTGAGTCATTT CCCAGTTCAGTAGCTGGATATGGAGACATTTACCTCAATGCTGGTCCCATGTTGTTTAGTGGAGACAAGG ACCATAGATTTGAATATAGCCATGGTCCTATTGCAGTCCTGGCAAACAGCAGTGACCCTTCCACGGGGCC AGAGAGTACTCATCCTTTGCCAGCAAAGATGCACAACTATAACTATGGTGGTAACTTACAGGAAAATCCG AGTGGCCCCAGCCTCATGCATGGACAGACCTGGACTTCTCCTGCCCAAGGACCTGGATATTCACAAGGAT ACAGGGGACATATTAGCACATCAACTGGCAGAGGCAGAGGCAGAGGGTTACCATACTGA >OriGene 5' read for NM_003718 unedited
ACCGCCCGTTCGAGCAAAGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCATTTAGGT GACACTATAGAATACAAGCTACTTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGTGGCGCTTT TCCCGGCCGGCTCTGGTGCTCGGTGTCCCTCCGCCGCCGCTCCCGTTTCCGGCGGGGGAGATGGCCAGGA TCTGACCCGGGAGGAGGCCGCACCCGCGCCGCGCTCTGCGGCTGGCTCTAGGCGATGCCGAGCAGCTCGG ACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCGGAGAAGAAGTTGGAGGAACGCCGCAAGCGGAG GCGATTCCTGTCCCCTCGGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCAGCTCCTGCGGGCGCCG CCGCCCCCGCCGCCTCTGCTCTTCGTGGCTGCTGCCGGCACGGCCGCCCGCCACAGCCACCGCTGCCACC GTTCAAGAATCGTAATTCTGCCCCCAGAGCCCTCAAATTATAAACGAACATGGACGGCCGTCAGTAAAGC TGATTTCGAACTACCATGCCTTGGACGGAGTGGGAGGAGCAGTTTCTCATAAGTTTAAACATGGCTCCAT CATATTAATGCGGACATTTTACAAGGATGTTAACTCCTCTCACCCGTATAGTTGAACAAGAAATAGCCCA TTAGCAGAATTACGTCAGATCATACTACGAGACGCCCGCCCGAA >SC323704 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GTARCTTTAGTCACTCTACTTACCACCGTTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGAAGCTGAT AGCTTACGAGGAAATATTTCAGTAAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTTCTTG CTGATTTACCGCTGCCCCCTGAGCTACCAGGAGGAGATGATCTTTCAAAGAGTCCAGAGGAAAAGAAAAC AGCAACACAGTTACATAGTAAAAGGAGGCCTAAAATATGTGGGCC |
Restriction Sites | Please inquire |
ACCN | NM_003718 |
Insert Size | 3500 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003718.2, NP_003709.2 |
RefSeq Size | 5246 bp |
RefSeq ORF | 4539 bp |
Locus ID | 8621 |
UniProt ID | Q14004 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | The protein encoded by this gene is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. The exact function of this protein has not yet been determined, but it may play a role in mRNA processing and may be involved in regulation of hematopoiesis. Alternatively spliced transcript variants have been described.[provided by RefSeq, Dec 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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SDS |
Resources
Other Versions
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RC217081 | CDK13 (Myc-DDK-tagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 10,344.00 |
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RC217081L1 | Lenti-ORF clone of CDK13 (Myc-DDK-tagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 12,744.00 |
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RC217081L2 | Lenti-ORF clone of CDK13 (mGFP-tagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 17,010.00 |
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RC217081L3 | Lenti-ORF clone of CDK13 (Myc-DDK-tagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 17,010.00 |
|
RC217081L4 | Lenti-ORF clone of CDK13 (mGFP-tagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 17,010.00 |
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RG217081 | CDK13 (tGFP-tagged) - Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 16,630.00 |
|
SC109966 | CDK13 (untagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1 |
CNY 10,352.00 |