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GRK6 (NM_002082) Human Untagged Clone
CAT＃: SC323679
GRK6 (untagged)-Kinase deficient mutant (K215M) of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2
CNY 6,592.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPRK6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323679 sequence for NM_002082 edited (data generated by NextGen Sequencing)
ATGGAGCTCGAGAACATCGTAGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGCGGT GGAAATCGCAAAGGCAAAAGCAAGAAATGGCGGCAGATGCTCCAGTTCCCTCACATCAGC CAGTGCGAAGAGCTGCGGCTCAGCCTCGAGCGTGACTATCACAGCCTGTGCGAGCGGCAG CCCATTGGGCGCCTGCTGTTCCGAGAGTTCTGTGCCACGAGGCCGGAGCTGAGCCGCTGC GTCGCCTTCCTGGATGGGGTGGCCGAGTATGAAGTGACCCCGGATGACAAGCGGAAGGCA TGTGGGCGGCAGCTAACGCAGAATTTTCTGAGCCACACGGGTCCTGACCTCATCCCTGAG GTCCCCCGGCAGCTGGTGACGAACTGCACCCAGCGGCTGGAGCAGGGTCCCTGCAAAGAC CTTTTCCAGGAACTCACCCGGCTGACCCACGAGTACCTGAGCGTGGCCCCTTTTGCCGAC TACCTCGACAGCATCTACTTCAACCGTTTCCTGCAGTGGAAGTGGCTGGAAAGGCAGCCA GTGACCAAAAACACCTTCAGGCAATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTG TGCGCCTGCCAGGTGCGGGCCACAGGTAAGATGTATGCCTGCATGAAGCTAGAGAAAAAG CGGATCAAGAAGCGGAAAGGGGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAA GTGAACAGTAGGTTTGTAGTGAGCTTGGCCTACGCCTATGAGACCAAGGACGCGCTGTGC CTGGTGCTGACACTGATGAACGGGGGCGACCTCAAGTTCCACATCTACCACATGGGCCAG GCTGGCTTCCCCGAAGCGCGGGCCGTCTTCTACGCCGCCGAGATCTGCTGTGGCCTGGAG GACCTGCACCGGGAGCGCATCGTGTACAGGGACCTGAAGCCCGAGAACATCTTGCTGGAT GACCACGGCCACATCCGCATCTCTGACCTGGGACTAGCTGTGCATGTGCCCGAGGGCCAG ACCATCAAAGGGCGTGTGGGCACCGTGGGTTACATGGCTCCGGAGGTGGTGAAGAATGAA CGGTACACGTTCAGCCCTGACTGGTGGGCGCTCGGCTGCCTCCTGTACGAGATGATCGCA GGCCAGTCGCCCTTCCAGCAGAGGAAGAAGAAGATCAAGCGGGAGGAGGTGGAGCGGCTG GTGAAGGAGGTCCCCGAGGAGTATTCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGC TCACAGCTCCTCTGCAAGGACCCTGCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGC GAGGTGAAGGAGCACCCCCTCTTTAAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATG CTGGAGCCGCCCTTCAAGCCTGACCCCCAGGCCATTTACTGCAAGGATGTTCTGGACATT GAACAGTTCTCTACGGTCAAGGGCGTGGAGCTGGAGCCTACCGACCAGGACTTCTACCAG AAGTTTGCCACAGGCAGTGTGCCCATCCCCTGGCAGAACGAGATGGTGGAGACCGAGTGC TTCCAGGAGCTGAATGTCTTTGGGCTGGATGGCTCAGTTCCCCCAGACCTGGACTGGAAG GGCCAGCCACCTGCACCTCCTAAAAAGGGACTGCTGCAGAGACTCTTCAGTCGCCAANNG ATTGCTGTGGAAACTGCAGCGACAGCGAGGAAGAGCTCCCCACCCGCCTCTAGCCCCCAG CCCGAGGCCCCCACCAGCAGTTGGCGGTAG Clone variation with respect to NM_002082.3 644 a=>t;1392 g=>c;1566 a=>g;1678 a=>n;1679 g=>n >OriGene 5' read for mutant NM_002082 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGCCGGGGAGG CCGCGGCGCGGTCACTGCGAGCCGAGCCGAGCCGCGCCGAGCCGCGCCGATCGCCATCCGGCCTCGGCAC TCGCGCGCGATCCCGGCCGGCGGCGCGGCCCGGCGGGCCAGGCGGCGCCACAGCCCATGGAGCTCGAGAA CATCGTAGCGAACACGTGCTACTCAAGCCCCGGGAAGGTGGCGGGTGGAAATCGCAAAAGGCAAAAGCAA GAAATGGCGGGCAGAATGGCTTCCAGTTTCCCTCACATCAAGCCCAGTGCCGAAAAAGCTGCGCGGCTCC AGCCTCCGAGCGCGTGACTATATCACAGCTGGGGCCAAGCCGGCACCCAATTTGGCCCGCTGTGCTGGTT CGAAAGATTTTTGGGCCACAAGGCGGGACCCTAACCCCCCTGCTTCCCTTTTCTGGGATGGGGGGGGCCC AATTGAAATGAGCCCCGGAGGAAAAAGCCGGAAGGGTTTGGGGGGGGAACCTAACCCCAAATTTTTTCAA GCCAACAGGGCCCTGACCCCTACCCCGAGGGTCCCCCCGGCACTGGGGAAAAAAATGAAACAAAGGGGTG GGGCAAGGGGCCTGGCGAGAACATTTTTCCGAAAAATAAACGGGGGAAAAAAGAATACCCGAGCGTGGGG CCCTTTTTTCGCCAACCTCCGCCAGAAGCTTCTTTTTCCGCTTTTTTGCAGTGAAAATACTCGAAAAGAA GCCCCTGTGCAAAAAAACACTCTTGGCGGAACTACTCCTCTGCGAAAAAGGCTTTTTGGAAGAGTGTTCC TCCCATGCTGCCCACACAAAAAATATTATCCCTCTATCCACTATAAAACCATATTATCATACGATAAGAG GGGGTGCGTCACTGCGCATCACGACAAACATTGCTATA >SC323679 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTTYCGACMGTGMAAAACACCTTCAGGCATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTGTGCGC CTGCCAGGTGCGGGCCACAGGTAAGATGTATGCCTGCATGAAGCTAGAGAAAAAGCGGATCAAGAAGCGG AAAGGGGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAAGTGAACAGTAGGTTTGTAGTGAGCT TGGCCTACGCCTATGAGACCAAGGACGCGCTGTGCCTGGTGCTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_002082 |
| Insert Size | 2880 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002082.2, NP_002073.2 |
| RefSeq Size | 2932 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 2870 |
| UniProt ID | P43250 |
| Domains | RGS, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Endocytosis |
| Gene Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (B). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218924 | GRK6 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2 |
CNY 1,800.00 |
|
| RC218924L1 | Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC218924L2 | Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC218924L3 | Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC218924L4 | Lenti ORF clone of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG218924 | GRK6 (tGFP-tagged) - Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2 |
CNY 3,400.00 |
|
| SC108487 | GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2 |
CNY 6,592.00 |
