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LIM kinase 2 (LIMK2) (NM_016733) Human Untagged Clone
CAT＃: SC323676
LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2b
CNY 4,600.00
CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323676 sequence for NM_016733 edited (data generated by NextGen Sequencing)
ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCA GAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCC AAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGG CCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGC AAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGT GGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCT GTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGC AGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAA GTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGG GACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAG GATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTCTCC CAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCCGGA CACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGGAGA CGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAG CCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTAT TCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTC TTTGGGCAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAG TTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGC AGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAGCTG AACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATGGAT CCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTAT TTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTG GACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAA AGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAG AAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGAAAG AGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATTGGG CAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTGAAG CTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCC ATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTT GAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAG TTGGACCACACTGTGAGCATGCAGTACGGCCTGACCCGGGACTCACCTCCCTAG Clone variation with respect to NM_016733.2 >OriGene 5' read for mutant NM_016733 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGCGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAATGAGAACATA CAGCCGAGAATACTGCCGAAGCTGAGACTGACTACTGTGCATTAGGAAAGACCTGGAGTCAGGACTTTGG TGGGATTTGGAGCTCCGAGGCAGTAATAACTGAACAAGCAGCCCTGTCCCCTAGGCTGCAGAAGCTTGAA TGCATCCTCTCCCAGAACCTGCCACAGGAAACTGGGGGCTTTGTCAGGTGAAGATGTCTGGAGGGTGTCC AGGCTGTGGGGACCACATTTGCTCCAAGCCAGATATGGGTACAGGACTGGTCAACGAAACCTGGGCACGG CTCTTGCTTCGGGGTTCAGAATGCCAGGATTCCCTCACAACTGGGTACTATGAGAAGGATGGGAAGCTTT ACTGCCCAGGACTACTGGGGGAAGTTTGGGGAGTCGGCCTGGGTGCTCCTCTGATGACGGGCCTTTTTGG TGGCCTGGGGGAGTCAGTTACCCCCCAAGGCTTTCCTGTTGACCTGCAGGAATATGGGAGAGGGAATGCT TTGATGGGTGCACATGCCACTCTCTCTGTGGAAGTCCAAGAGTGTGGCGTGCCACTGTTTGAGAAACTCC CAGTTTGTTCGAGACCTCCCTATTGTACCTAATCTAGCGCGCCACTTAGACGACGCGGCTTCTGGGTCGG GGAAGCGTTGCCATCACCCCACATTGACCTGAAGTTAGCAGTAACAATTCCACTGAGCTCCTGGACCATC GTATGACCCCTGGCCACTCTGAGAAGAGACTCT >SC323676 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGAWCTTCGACTGTGACTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTG ACACACAAAGCCACGGGCAAAGTGATGGTCATGATGGAGTTAATTCGATGTGATGAGGAGACCCAGAAAA CTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCT GTACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_016733 |
| Insert Size | 4130 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016733.2, NP_057952.1 |
| RefSeq Size | 3848 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 3985 |
| UniProt ID | P53671 |
| Domains | pkinase, TyrKc, PDZ, LIM, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2b) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (2b) is shorter and has a distinct C-terminus compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220400 | LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2b |
CNY 4,592.00 |
|
| RC220400L3 | Lenti ORF clone of Human LIM domain kinase 2 (LIMK2), transcript variant 2b, Myc-DDK-tagged |
CNY 7,320.00 |
|
| RC220400L4 | Lenti ORF clone of Human LIM domain kinase 2 (LIMK2), transcript variant 2b, mGFP tagged |
CNY 7,320.00 |
|
| RG220400 | LIMK2 (tGFP-tagged) - Human LIM domain kinase 2 (LIMK2), transcript variant 2b |
CNY 6,192.00 |
|
| SC127711 | LIMK2 (untagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2b |
CNY 4,600.00 |
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