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DCLK3 (NM_033403) Human Untagged Clone
CAT＃: SC323628
DCLK3 (untagged)-Kinase deficient mutant (K313M) of Human doublecortin-like kinase 3 (DCLK3)
CNY 4,832.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLR; DCAMKL3; DCDC3C; DCK3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323628 sequence for NM_033403 edited (data generated by NextGen Sequencing)
ATGGGCAAAGAACCACTGACACTGAAGAGCATTCAGGTGGCTGTAGAAGAACTGTACCCC AACAAAGCCCGGGCCCTGACACTGGCCCAGCACAGCCGTGCCCCTTCTCCAAGGCTGAGG AGCAGGCTGTTTAGCAAGGCTCTGAAAGGAGACCACCGCTGTGGGGAGACCGAGACCCCC AAGAGCTGCAGCGAAGTTGCAGGATGCAAGGCAGCCATGAGGCACCAGGGGAAGATCCCC GAGGAGCTTTCACTAGATGACAGAGCGAGGACCCAGAAGAAGTGGGGGAGGGGGAAATGG GAGCCAGAACCCAGTAGCAAGCCCCCCAGGGAAGCCACTCTGGAAGAGAGGCACGCAAGG GGAGAGAAGCATCTTGGGGTGGAGATTGAAAAGACCTCGGGTGAAATTATCAGATGCGAG AAGTGCAAGAGAGAGAGGGAGCTCCAGCAGAGCCTGGAGCGTGAGAGGCTTTCTCTGGGG ACCAGTGAGCTGGATATGGGGAAGGGCCCAATGTATGATGTGGAGAAGCTGGTGAGGACC AGAAGCTGCAGGAGGTCTCCCGAGGCAAATCCTGCAAGTGGGGAGGAAGGGTGGAAGGGT GACAGCCACAGGAGCAGCCCCAGGAATCCCACTCAAGAGCTGAGGAGACCCAGCAAGAGC ATGGACAAGAAAGAGGACAGAGGCCCAGAGGATCAAGAAAGCCATGCTCAGGGAGCAGCC AAGGCCAAGAAGGACCTTGTGGAAGTTCTTCCTGTCACAGAGGAGGGGCTGAGGGAGGTG AAGAAGGACACCAGGCCCATGAGCAGGAGCAAACATGGTGGCTGGCTCCTGAGAGAGCAC CAGGCGGGCTTTGAGAAGCTCCGCAGGACCCGAGGAGAAGAGAAGGAGGCAGAGAAGGAG AAAAAGCCATGTATGTCTGGAGGCAGAAGGATGACTCTCAGAGATGACCAACCTGCAAAG CTAGAAAAGGAGCCCAAGACGAGGCCAGAAGAGAACAAGCCAGAGCGGCCCAGCGGTCGG AAGCCACGGCCCATGGGCATCATTGCCGCCAATGTGGAAAAGCATTATGAGACTGGCCGG GTCATTGGGGATGGGAACTTTGCTGTCGTGAAGGAGTGCAGACACCGCGAGACCAGGCAG GCCTATGCGATGATGATCATTGACAAGTCCAGACTCAAGGGCAAGGAGGACATGGTGGAC AGTGAGATCTTGATCATCCAGAGCCTCTCTCACCCCAACATCGTGAAATTGCATGAAGTC TACGAAACAGACATGGAAATCTACCTGATCCTGGAGTACGTGCAGGGAGGAGACCTTTTT GACGCCATCATAGAAAGTGTGAAGTTCCCGGAGCCCGATGCTGCCCTCATGATCATGGAC TTATGCAAAGCCCTCGTCCACATGCACGACAAGAGCATTGTCCACCGGGACCTCAAGCCG GAAAACCTTTTGGTTCAGCGAAATGAGGACAAATCTACTACCTTGAAATTGGCTGATTTT GGACTTGCAAAGCATGTGGTGAGACCTATATTTACTGTGTGTGGGACCCCAACTTACGTA GCTCCCGAAATTCTTTCTGAGAAAGGTTATGGACTGGAGGTGGACATGTGGGCTGCTGGC GTGATCCTCTATATCCTGCTGTGTGGCTTTCCCCCATTCCGCAGCCCTGAGAGGGACCAG GACGAGCTCTTTAACATCATCCAGCTGGGCCACTTTGAGTTCCTCCCCCCTTACTGGGAC AATATCTCTGATGCTGCTAAAGATCTGGTGAGCCGGTTGCTGGTGGTAGACCCCAAAAAG CGCTACACAGCTCATCAGGTTCTTCAGCACCCCTGGATCGAAACAGCTGGCAAGACCAAT ACAGTGAAACGACAGAAGCAGGTGTCCCCCAGCAGCGAGGGTCACTTCCGGAGCCAGCAC AAGAGGGTTGTGGAGCAGGTATCATAG Clone variation with respect to NM_033403.1 1154 a=>t >OriGene 5' read for mutant NM_033403 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGCCTTCACTCA GCACATCCTGAGGACAGCCCTCTGAAGCCCAGGGTCGTGACCGTAGTGAAGCTGGGTGGGCAGCGCCCCC GAAAGATCACTCTGCTCCTCAACAGGCGATCAGTGCAGACGTTCGAGCAGCTCTTAGCTGACATCTCAGA AGCCTTGGGCTCTCCCAGATGGAAGAATGACCGTGTGAGGAAACTGTTTAACCTCAAGGGGCAGGGAAGT CAGGGAGCGTCTCTGATTTCTTCAGGGAAGGGGATGCTTTTCATAGCTATGGGCAAAGACCACTGACCAC TGAAGAGCATTCAGGTGGGCTTGTGGAAGACTGGTACCCCACCAAAGCCCCGGGCCCTGGACCTGCCCCG GCACAGCCGTGCCCTTTTCCAAGGCTGAGGAGCAGGCTTGTTTACCAGGGCTCTAAAGGGAAACAACCGC GTGGGGGAGACCAAAACCCCAAACCTGCACCAAATTTGAGGATGCAGGGCACCCTTGAGGCCCAGGGAAA AATCCCAGAGAGCTTTACTTAATGAGACAGACCGGGACCCGAGAAATGGGAGGGGGAAAATGGGCCCTAA CCCTGACCAGCCCCCAAGAGACCACTCTGAAAAGGCACCAGGGGAGAAACCCTCTGGGTGGAATTGAGAG ACTCGTGGAAATTCTATGCAAGTGCCGAGAAGAGCTCCCGACCTGCCGTAGCTCTTGGGCATAGCGGATT GGAAGCCAGTGTATGTGAACTTGTACTAGGCGGAGGTGT >SC323628 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGAAGATCATTGGGGWGGGACTTTGCTGTCGTGAAGGAGTGCAGACACCGCGAGACCAGGCAGGCCTATG CGATGATGATCATTGACAAGTCCAGACTCAAGGGCAAGGAGGACATGGTGGACAGTGAGATCTTGATCAT CCAGAGCCTCTCTCACCCCAACATCGTGAAATTGCATGAAGTCTACGAAACAGACATGGAAATCTACCTG ATCCTGGAGTACGTGCAGGGAGGAGACCTTTTTGACGCCATCATA |
| Restriction Sites | Please inquire |
| ACCN | NM_033403 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033403.1, NP_208382.1 |
| RefSeq Size | 5344 bp |
| RefSeq ORF | 1947 bp |
| Locus ID | 85443 |
| UniProt ID | Q9C098 |
| Protein Families | Druggable Genome, Protein Kinase |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213096 | DCLK3 (Myc-DDK-tagged)-Human doublecortin-like kinase 3 (DCLK3) |
CNY 4,824.00 |
|
| RC213096L1 | Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), Myc-DDK-tagged |
CNY 7,224.00 |
|
| RC213096L2 | Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), mGFP tagged |
CNY 6,460.00 |
|
| RC213096L3 | Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC213096L4 | Lenti ORF clone of Human doublecortin-like kinase 3 (DCLK3), mGFP tagged |
CNY 6,460.00 |
|
| RG213096 | DCLK3 (tGFP-tagged) - Human doublecortin-like kinase 3 (DCLK3) |
CNY 5,040.00 |
|
| SC316560 | DCLK3 (untagged)-Human doublecortin-like kinase 3 (DCLK3) |
CNY 10,360.00 |
