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CAMK2G (NM_172170) Human Untagged Clone
CAT＃: SC323611
CAMK2G (untagged)-Kinase deficient mutant (K43M) of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3
CNY 3,864.00
CNY 8,360.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAMK; CAMK-II; CAMKG; MRD59 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_172170, the custom clone sequence may differ by one or more nucleotides
ATGGCCACCACCGCCACCTGCACCCGTTTCACCGACGACTACCAGCTCTTCGAGGAGCTTGGCAAGGGTG CTTTCTCTGTGGTCCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAGTACGCAGCAAAAATCATCAATAC CAAGAAGTTGTCTGCCCGGGATCACCAGAAACTAGAACGTGAGGCTCGGATATGTCGACTTCTGAAACAT CCAAACATCGTGCGCCTCCATGACAGTATTTCTGAAGAAGGGTTTCACTACCTCGTGTTTGACCTTGTTA CCGGCGGGGAGCTGTTTGAAGACATTGTGGCCAGAGAGTACTACAGTGAAGCAGATGCCAGCCACTGTAT ACATCAGATTCTGGAGAGTGTTAACCACATCCACCAGCATGACATCGTCCACAGGGACCTGAAGCCTGAG AACCTGCTGCTGGCGAGTAAATGCAAGGGTGCCGCCGTCAAGCTGGCTGATTTTGGCCTAGCCATCGAAG TACAGGGAGAGCAGCAGGCTTGGTTTGGTTTTGCTGGCACCCCAGGTTACTTGTCCCCTGAGGTCTTGAG GAAAGATCCCTATGGAAAACCTGTGGATATCTGGGCCTGCGGGGTCATCCTGTATATCCTCCTGGTGGGC TATCCTCCCTTCTGGGATGAGGATCAGCACAAGCTGTATCAGCAGATCAAGGCTGGAGCCTATGATTTCC CATCACCAGAATGGGACACGGTAACTCCTGAAGCCAAGAACTTGATCAACCAGATGCTGACCATAAACCC AGCAAAGCGCATCACGGCTGACCAGGCTCTCAAGCACCCGTGGGTCTGTCAACGATCCACGGTGGCATCC ATGATGCATCGTCAGGAGACTGTGGAGTGTTTGCGCAAGTTCAATGCCCGGAGAAAACTGAAGGGTGCCA TCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGG CGGTGTCAAGCCACAGAGCAACAACAAAAACAGTCTCGTAAGCCCAGCCCAAGAGCCCGCGCCCTTGCAG ACGGCCATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCT GCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCT GATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTT GAGCCTGAGGCCCTTGGTAACCTCGTGGAGGGGATGGATTTCCATAAGTTTTACTTTGAGAATCTCCTGT CCAAGAACAGCAAGCCTATCCATACCACCATCCTAAACCCACACGTCCACGTGATTGGGGAGGACGCAGC GTGCATCGCCTACATCCGCCTCACCCAGTACATCGACGGGCAGGGTCGGCCTCGCACCAGCCAGTCAGAA GAGACCCGGGTCTGGCACCGTCGGGATGGCAAGTGGCTCAATGTCCACTATCACTGCTCAGGGGCCCCTG CCGCACCGCTGCAGTGA >OriGene 5' read for mutant NM_172170 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGGCGCGGGCT GCACCGCCGCGTCCGCCCGCCCGCCAGCATGGCCACCACCGCCACCTGCACCCGTTTCACCGACGACTAC CAGCTCTTCGAGGAGCTTGGCAAGGGTGCTTTCTCTGTGGTCCGCAGGTGTGTGAAGAAAACCTCCACGC AGGAGTACGCAGCAATGATCATCAATACCAAGAAGTTGTCTGCCCGGGATCACCCAGAAAACTAGAACGG GGAGGCTCGGATATGTCGACTTCTGAAAACATCCAAAACATCGGTGCCGCCTCCCATGAACAGTTATTTC TTGAAGAAGGGTTTCACTACCTTGTTGTTTTGACCTTGTTACCGGCGGGGAAGCTTGTTTGGAAGACTTT GTGGCCAGAGAGTACTACAGTGAACAGGATGCCAGCCCTGGTATACTTCGATTCTGGGAGAGTGTTAACC CCTTCCCCCAGATTGACTTTGTCCCAGGGACCTGACCTTGAGAACGGGTTGCGGGCGATTAAAGGCAGGG GTGCGCCCTAAGCTGGCTGATTTGGCCTACCCTCAATTACGGGGAGAGCACGCGCTTGTTGGTTGTGTGG CACCAGTCTACTTGCCCGAGTGTGTGGAGAGCTCCTGGGAACCCTGTGAATCTGGCTCGCGGCATCTCTG TAACCCCGTGGCGACATCTCTGGGAGAGATACACACACGTTCACTAACGGGGCCCTATCTCCCCCATGAG CAGTATCTGGACGCAATGTTACACGGGCTATCAGACATCTGTCCACTTTAACCTGATGTAA >SC323611 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AYRTTMMRMAAWGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACMRK CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGGCGCGGGCTGCACC GCCGCGTCCGCCCGCCCGCCAGCATGGCCACCACCGCCACCTGCACCCGTTTCACCGACGACTACCAGCT CTTCRAGGAGCTTGGCAWGGGTGCTTTCTCTGTGGTCCGCAGGTG |
| Restriction Sites | Please inquire |
| ACCN | NM_172170 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172170.2, NP_751910.1 |
| RefSeq Size | 3712 bp |
| RefSeq ORF | 1557 bp |
| Locus ID | 818 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway |
| Gene Summary | The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.[provided by RefSeq, Mar 2011] Transcript Variant: This variant (3) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (3), that is missing an internal segment compared to isoform 1. Variants 3 and 50 both encode the same isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222351 | CAMK2G (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3 |
CNY 3,856.00 |
|
| RC222351L1 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3, Myc-DDK-tagged |
CNY 6,256.00 |
|
| RC222351L2 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3, mGFP tagged |
CNY 6,370.00 |
|
| RC222351L3 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC222351L4 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3, mGFP tagged |
CNY 6,370.00 |
|
| RG222351 | CAMK2G (tGFP-tagged) - Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3 |
CNY 5,456.00 |
|
| SC100733 | CAMK2G (untagged)-Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3 |
CNY 3,864.00 |
|
| SC323621 | CAMK2G (untagged)-Kinase deficient mutant (K43M) of Human calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 3 |
CNY 3,864.00 |
