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PRPK (TP53RK) (NM_033550) Human Untagged Clone
CAT＃: SC323584
TP53RK (untagged)-Kinase deficient mutant (D162A) of Human TP53 regulating kinase (TP53RK)
CNY 2,400.00
CNY 3,990.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK; TPRKB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323584 sequence for NM_033550 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGGCTGAG GCTCTGGCCGCAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAG CAGGGTGCCGAGGCGCGCGTGTTCCGTGGCCGCTTCCAGGGCCGCGCGGCGGTGATCAAG CACCGCTTCCCCAAGGGCTACCGGCACCCGGCGCTGGAGGCGCGGCTTGGCAGACGGCGG ACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCGCTGGAATATCTGCCCCAGTT GTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGAAGGCTCAGTG ACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTCTCTCC AACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCATTCAT GGTGCCCTCACCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACATTGTG CTCATAGACTTTGGGCTGAGTTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAGACCTC TATGTCCTGGAGAAGGCCTTCCTCAGTACCCATCCCAACACTGAAACTGTGTTTGAAGCC TTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGGCCAGGCCAGTGCTAAAAAAATTAGAT GAAGTGCGCCTGAGAGGAAGAAAGAGGTCCATGGTTGGGTAG Clone variation with respect to NM_033550.3 485 a=>c;486 t=>c >OriGene 5' read for mutant NM_033550 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTAACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGATCGGTTGGAG CTGTTGCGCCGAGCAGTCATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGG AGGCTGAGGCTCTGGCCGCAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAGCA GGGTGCCGAGGCGCGCGTGTTCCGTGGCCGCTTCCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCC AAGGGCTACCGGCACCCGGCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGAGGCCCGGGCGCT CCTCCGCTGTCGCCGCGCTGGATATCTGCCCCAGTTGTCTTTTTGTGGACTATGCTTCCAACTGCTTATA TATGGAGAATTGAAGGCTCAGTGACTGTTCGAAGATTATATTCAGTCCACTATGGAAGACTGAAAAAACC TCCCAGGTTCTCTCACTTAGCCAAGACAATTGGGCCAGTTTTGCTTCGAATGCACCGAATGAAAGACCTC ATCATGGTGCCCTACCCACCCTCCAACATTGCCTTCTGAGCCCCCTTGGACCAGGCTGAACCATTTGGTC TTATAGACTAGGCCTGATCCATCGCAGCACTCCAGAGTTTGGAATTGAGACCTTATGTCTCTGGAAGGCC TTCTGATCCATTCCCACCATGGACTGTTTGACCCTTCGGAGGACCTCTACACTCTCTCAAGCACGCATGT CAGAAATATCGAATTGCCTGGAGAAAGTCTCAGTGGTCTAGTGTAACCCAACTGGACTTCCCGA >SC323584 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ASCCAKTTCGTGACGCTTCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCCAAGGGCTACCSSYWCC CGGCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCG CGCTGGAATATCTGCCCCAGTTGTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATT GAAGGCTCAGTGACTGTTCGAGATTATATTCAGTCCACTATGGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_033550 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033550.3, NP_291028.3 |
| RefSeq Size | 3384 bp |
| RefSeq ORF | 762 bp |
| Locus ID | 112858 |
| UniProt ID | Q96S44 |
| Domains | S_TKc, KOW |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209748 | TP53RK (Myc-DDK-tagged)-Human TP53 regulating kinase (TP53RK) |
CNY 2,400.00 |
|
| RC209748L1 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC209748L2 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), mGFP tagged |
CNY 5,890.00 |
|
| RC209748L3 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC209748L4 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), mGFP tagged |
CNY 4,800.00 |
|
| RG209748 | TP53RK (tGFP-tagged) - Human TP53 regulating kinase (TP53RK) |
CNY 4,000.00 |
|
| SC120376 | TP53RK (untagged)-Human TP53 regulating kinase (TP53RK) |
CNY 2,400.00 |
