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DYRK2 (NM_006482) Human Untagged Clone
CAT＃: SC323580
DYRK2 (untagged)-Kinase deficient mutant (K251M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2
CNY 4,480.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323580 sequence for NM_006482 edited (data generated by NextGen Sequencing)
ATGTTAACCAGGAAACCTTCGGCCGCCGCTCCCGCCGCCTACCCGACCGGCCGAGGTGGG GACAGCGCCGTTCGTCAGCTTCAGGCTTCCCCGGGGCTCGGTGCAGGGGCCACCCGGAGC GGAGTGGGGACTGGCCCGCCCTCCCCCATCGCCCTGCCGCCTCTCCGGGCCAGCAACGCT GCCGCCGCAGCCCACACGATTGGCGGCAGTAAGCACACAATGAATGATCACCTGCATGTC GGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTGTTTGAGGATAACAGTAACAAG CGGACAGTGCTCACGACACAACCAAATGGGCTTACAACAGTGGGCAAAACGGGCTTGCCA GTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGGCAGGGGAGCTCCACCTCTCTA AAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCCATGACACCTGAACAAGCAATG AAGCAATACATGCAAAAACTCACAGCCTTCGAACACCATGAGATTTTCAGCTACCCTGAA ATATATTTCTTGGGTCTAAATGCTAAGAAGCGCCAGGGCATGACAGGTGGGCCCAACAAT GGTGGCTATGATGATGACCAGGGATCATATGTGCAGGTGCCCCACGATCACGTGGCTTAC AGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGGCCTAC GATCACAAAGTCCACCAGCACGTGGCCCTAATGATGGTGCGGAATGAGAAGCGCTTCCAC CGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAAC ACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG TTTGAGCTGCTGAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAATTCCAGGGCTTC AGTCTGCCTTTGGTTCGCAAGTTTGCCCACTCGATTCTGCAGTGCTTGGATGCTTTGCAC AAAAACAGAATAATTCACTGTGACCTTAAGCCCGAGAACATTTTGTTAAAGCAGCAGGGT AGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGTTACGAGCATCAGCGTGTCTAC ACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTGATCCTTGGGGCCAGGTATGGC ATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCAGAGCTCCTGACGGGTTACCCC CTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGTATGATTGAACTGTTGGGCATG CCCTCACAGAAACTGCTGGATGCATCCAAACGAGCCAAAAATTTTGTGAGCTCCAAGGGT TATCCCCGTTACTGCACTGTCACGACTCTCTCAGATGGCTCTGTGGTCCTAAACGGAGGC CGTTCCCGGAGGGGGAAACTGAGGGGCCCACCGGAGAGCAGAGAGTGGGGGAACGCGCTG AAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAACAGTGTTTAGAGTGGGATCCT GCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCCTGGCTGAGGAGGCGGTTGCCA AAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATAACTGAGAGCACCGGTGCTATC ACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGACTAATTTG GCGCAGATGACAGATGCCAATGGGAATATTCAGCAGAGGACAGTGTTGCCAAAACTTGTT AGCTGA Clone variation with respect to NM_006482.2 752 a=>t >OriGene 5' read for mutant NM_006482 unedited
CCGCCCGTTACAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGAGCGGGGG GCTCGCGGCGGCGGGCCCCGGCCGAGGGGATGCAGTGGACTGTGTGTGTCTGGCTGTAGCAGACGCGAGG CGGCGACGAGGCGCCGGGGACCCGCGCGAGGGGCGGCCGGGAGGCGGCGGCGGCGGCCGCCAGAGTAGCA GCAGACCGGCGGCGGCGACGGCAGCCCTGAATGCATTTTCCTCTCCACGCCCCGTGGGGGGGGGGGGCGG AGGCCGCGCTACGCCGCTAACCGACCGCCGGGTGTGACACCGCGTTCGGGGGGTGCGGTTCCCGGGACTC GTGATGAACCACAAAAAATAAATG >SC323580 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TAGTATMMGTGGCTTAAGGTATGAGGTCCTCAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGG CCTACGATCACAAAGTCCACCAGCACGTGGCCCTAATGATGGTGCGGAATGAGAAGCGCTTCCACCGGCA AGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAACACAATGAATGTCATC CATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG |
| Restriction Sites | Please inquire |
| ACCN | NM_006482 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006482.1, NP_006473.1 |
| RefSeq Size | 3615 bp |
| RefSeq ORF | 1806 bp |
| Locus ID | 8445 |
| UniProt ID | Q92630 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) includes the 149 bp insert in the 5' end of the transcript and thus results in a longer amino-terminal protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202864 | DYRK2 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2 |
CNY 4,472.00 |
|
| RC202864L3 | Lenti-ORF clone of DYRK2 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2 |
CNY 6,460.00 |
|
| RC202864L4 | Lenti-ORF clone of DYRK2 (mGFP-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2 |
CNY 6,460.00 |
|
| RG202864 | DYRK2 (tGFP-tagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2 |
CNY 6,072.00 |
|
| SC109929 | DYRK2 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 2 |
CNY 4,480.00 |
