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AKT2 (NM_001626) Human Untagged Clone
CAT＃: SC323570
AKT2 (untagged)-Kinase deficient mutant (K181M) of Human v-akt murine thymoma viral oncogene homolog 2 (AKT2)
CNY 5,488.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323570 sequence for NM_001626 edited (data generated by NextGen Sequencing)
ATGAATGAGGTGTCTGTCATCAAAGAAGGCTGGCTCCACAAGCGTGGTGAATACATCAAG ACCTGGAGGCCACGGTACTTCCTGCTGAAGAGCGACGGCTCCTTCATTGGGTACAAGGAG AGGCCCGAGGCCCCTGATCAGACTCTACCCCCCTTAAACAACTTCTCCGTAGCAGAATGC CAGCTGATGAAGACCGAGAGGCCGCGACCCAACACCTTTGTCATACGCTGCCTGCAGTGG ACCACAGTCATCGAGAGGACCTTCCACGTGGATTCTCCAGACGAGAGGGAGGAGTGGATG CGGGCCATCCAGATGGTCGCCAACAGCCTCAAGCAGCGGGCCCCAGGCGAGGACCCCATG GACTACAAGTGTGGCTCCCCCAGTGACTCCTCCACGACTGAGGAGATGGAAGTGGCGGTC AGCAAGGCACGGGCTAAAGTGACCATGAATGACTTCGACTATCTCAAACTCCTTGGCAAG GGAACCTTTGGCAGAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGCTACTACGCCATG ATGATCCTGCGGAAGGAAGTCATCATTGCCAAGGATGAAGTCGCTCACACAGTCACCGAG AGCCGGGTCCTCCAGAACACCAGGCACCCGTTCCTCACTGCGCTGAAGTATGCCTTCCAG ACCCACGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCAC CTGTCCCGGGAGCGTGTCTTCACAGAGGAGCGGGCCCGGTTTTATGGTGCAGAGATTGTC TCGGCTCTTGAGTACTTGCACTCGCGGGACGTGGTATACCGCGACATCAAGCTGGAAAAC CTCATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCCTCTGCAAAGAGGGC ATCAGTGACGGGGCCACCATGAAAACCTTCTGTGGGACCCCGGAGTACCTGGCGCCTGAG GTGCTGGAGGACAATGACTATGGCCGGGCCGTGGACTGGTGGGGGCTGGGTGTGGTCATG TACGAGATGATGTGCGGCCGCCTGCCCTTCTACAACCAGGACCACGAGCGCCTCTTCGAG CTCATCCTCATGGAAGAGATCCGCTTCCCGCGCACGCTCAGCCCCGAGGCCAAGTCCCTG CTTGCTGGGCTGCTTAAGAAGGACCCCAAGCAGAGGCTTGGTGGGGGGCCCAGCGATGCC AAGGAGGTCATGGAGCACAGGTTCTTCCTCAGCATCAACTGGCAGGACGTGGTCCAGAAG AAGCTCCTGCCACCCTTCAAACCTCAGGTCACGTCCGAGGTCGACACAAGGTACTTCGAT GATGAATTTACCGCCCAGTCCATCACAATCACACCCCCTGACCGCTATGACAGCCTGGGC TTACTGGAGCTGGACCAGCGGACCCACTTCCCCCAGTTCTCCTACTCGGCCAGCATCCGC GAGTGA Clone variation with respect to NM_001626.4 494 a=>g;542 a=>t >OriGene 5' read for mutant NM_001626 unedited
CCGCCCGTTGTAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAACACAAGGAA AGGGAACCAGCGCAGCGTGGCGATGGGCGGGGGTAGAGCCCCGCCGGAGAGGCTGGGCGGCTGCCGGTGA CAGACTGTGCCCTGTCCACGGTGCCTCCTGCATGTCCTGCTGCCCTGAGCTGTCCCGAGCTAGGTGACAG CGTACCACGCTGCCACCATGAATGAGGTGTCTGTCATCAAAGAAGGCTGGCTCCACAAGCGTGGTGAATA CATCCAAGACCTGGAGGCCACGGGTACTTTCCTGCTGAAAGAAGCGACGGCTTCCTTCATTGGGTCCAAG GAGAAGGCCGAAGGCCCCCTGGATCCAGAACTCTACCCCCCCTTAAACAACTTTTTCCGAACCAAATTGC CACCTGGAAGGAGAAACCGAAAGGCCCCGACCCACCCCCTTTTCTATACGCTGCTGCGATGTGGCACCAC AGCAATGAGAGAGGACCTTTCACCTGGATTTTCTCCACACAAAGGGAAGAGATGTGAATGCGGCCCATCG ATAGGGGCCCACACACCCTAACGCGGGGGCCCGCGCAGAGCCCCCTGGGCACACATGTGTGTCTCCCATG AGACTCTCCCCTCTGAGATGTGATGTGCGTCACAGGGCGGCAAGTGCACGATGAGACTACACTCAACCCT TGGGAGAGACCTTCGCAGATTCTCGTGTCGGAAGGCCTTGCCATATACCGTGATATCGCGAGAGATTCTG TCGCAGATATGTTCCAATTCTAGACGCCGTCTATCAAGCCCGCTCCG >SC323570 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ACTCACTCCTTGGCAGGGACCTTTGGCAGAGTCATCCTGGTGCGGGAGAAGGCCACTGGCCGCTACTACG CCATGATGATCCTGCGGAAGGAAGTCATCATTGCCAAGGATGAAGTCGCTCACACAGTCACCGAGAGCCG GGTCCTCCAGAACACCAGGCACCCGTTCCTCACTGCGCTGAAGTATGCCTTCCAGACCCACGACCGCCTG TGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTCTTCCAC |
| Restriction Sites | Please inquire |
| ACCN | NM_001626 |
| Insert Size | 1900 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001626.2, NP_001617.1 |
| RefSeq Size | 1715 bp |
| RefSeq ORF | 1446 bp |
| Locus ID | 208 |
| UniProt ID | P31751 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway |
| Gene Summary | This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217733 | AKT2 (Myc-DDK-tagged)-Human v-akt murine thymoma viral oncogene homolog 2 (AKT2) |
CNY 5,488.00 |
|
| RC217733L1 | Lenti ORF clone of Human v-akt murine thymoma viral oncogene homolog 2 (AKT2), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC217733L2 | Lenti ORF clone of Human v-akt murine thymoma viral oncogene homolog 2 (AKT2), mGFP tagged |
CNY 7,888.00 |
|
| RC217733L3 | Lenti ORF clone of Human v-akt murine thymoma viral oncogene homolog 2 (AKT2), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC217733L4 | Lenti ORF clone of Human v-akt murine thymoma viral oncogene homolog 2 (AKT2), mGFP tagged |
CNY 7,888.00 |
|
| RG217733 | AKT2 (tGFP-tagged) - Human v-akt murine thymoma viral oncogene homolog 2 (AKT2) |
CNY 7,088.00 |
|
| SC119161 | AKT2 (untagged)-Human v-akt murine thymoma viral oncogene homolog 2 (AKT2) |
CNY 5,488.00 |
