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Snf1lk (SIK1) (NM_173354) Human Untagged Clone
CAT＃: SC323562
SIK1 (untagged)-Kinase deficient mutant (K56M) of Human salt-inducible kinase 1 (SIK1)
CNY 5,744.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE30; MSK; SIK; SIK-1; SIK1B; SNF1LK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323562 sequence for NM_173354 edited (data generated by NextGen Sequencing)
ATGGTTATCATGTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAG CCCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGGCAACTTCGCGGTG GTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAATGATAATTGATAAA ACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGCTT CTGAACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATGCTTTAC ATCGTCACTGAATTTGCTAAAAATGGAGAAATGTTTGATTATTTGACTTCCAACGGGCAC CTGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGCAAATCCTGTCGGCCGTGGAGTACTGT CACGACCATCACATCGTCCACCGGGACCTCAAGACCGAGAACCTCCTGCTGGATGGCAAC ATGGACATCAAGCTGGCAGATTTTGGATTTGGGAATTTCTACAAGTCAGGAGAGCCTCTG TCCACGTGGTGTGGGAGCCCCCCGTATGCCGCCCCGGAAGTCTTTGAGGGGAAGGAGTAT GAAGGCCCCCAGCTGGACATCTGGAGCCTGGGCGTGGTGCTGTACGTCCTGGTCTGCGGT TCTCTCCCCTTCGATGGGCCTAACCTGCCGACGCTGAGACAGCGGGTGCTGGAGGGCCGC TTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGAGCCTGATCCGCCGCATGCTGGTG GTGGACCCCGCCAGGCGCATCACCATCGCCCAGATCCGGCAGCACCGGTGGATGCGGGCT GAGCCCTGCTTGCCGGGACCCGCCTGCCCCGCCTTCTCCGCACACAGCTACACCTCCAAC CTGGGCGACTACGATGAGCAGGCGCTGGGTATCATGCAGACCCTGGGCGTGGACCGGCAG AGGACGGTGGAGTCACTGCAAAACAGCAGCTATAACCACTTTGCTGCCATTTATTACCTC CTCCTTGAGCGGCTCAAGGAGTATCGGAATGCCCAGTGCGCCCGCCCCGGGCCTGCCAGG CAGCCGCGGCCTCGGAGCTCGGACCTCAGTGGTTTGGAGGTGCCTCAGGAAGGTCTTTCC ACCGACCCTTTCCGACCTGCCTTGCTGTGCCCGCAGCCGCAGACCTTGGTGCAGTCCGTC CTCCAGGCCGAGATGGACTGTGAGCTCCAGAGCTCGCTGCAGTGGCCCTTGTTCTTCCCG GTGGATGCCAGCTGCAGCGGAGTGTTCCGGCCCCGGCCCGTGTCCCCAAGCAGCCTGCTG GACACAGCCATCAGTGAGGAGGCCAGGCAGGGGCCGGGCCTAGAGGAGGAGCAGGACACG CAGGAGTCCCTGCCCAGCAGCACGGGCCGGAGGCACACCCTGGCCGAGGTCTCCACCCGC CTCTCCCCACTCACCGCGCCATGTATAGTCGTCTCCCCCTCCACCACGGCAAGTCCTGCA GAGGGAACCAGCTCTGACAGTTGTCTGACCTTCTCTGCGAGCAAAAGCCCCGCGGGGCTC AGTGGCACCCCGGCCACTCAGGGGCTGCTGGGCGCCTGCTCCCCGGTCAGGCTGGCCTCG CCCTTCCTGGGGTCGCAGTCCGCCACCCCAGTGCTGCAGGCTCAGGGGGGCTTGGGAGGA GCTGTTCTGCTCCCTGTCAGCTTCCAGGAGGGACGGCGGGCGTCGGACACCTCACTGACT CAAGGGCTGAAGGCCTTTCGGCAGCAGCTGAGGAAGACCACGCGTACCAAAGGGTTTCTG GGACTGAACAAAATCAAGGGGCTGGCTCGCCAGGTGTGCCAGGCCCCCGCCAGCCGGGCC AGCAGGGGCGGCCTGAGCCCCTTCCACGCCCCTGCACAGAGCCCAGGCCTGCACGGCGGC GCAGCCGGCAGCCGGGAGGGCTGGAGCCTGCTGGAGGAGGTGCTAGAGCAGCAGAGGCTG CTCCAGTTACAGCACCACCCGGCCGCTGCACCCGGCTGCTCCCAGGCCCCCCAGCCGGCC CCTGCCCCGTTTGTGATCGCCCCCTGTGATGGCCCTGGGGCTGCCCCGCTCCCCAGCACC CTCCTCACGTCGGGGCTCCCGCTGCTGCCGCCCCCACTCCTGCAGACCGGCGCGTCCCCG GTGGCCTCAGCGGCGCAGCTCCTGGACACACACCTGCACATTGGCACCGGCCCCACCGCC CTCCCCGCTGTGCCCCCACCACGCCTGGCCAGGCTGGCCCCAGGTTGTGAGCCCCTGGGG CTGCTGCAGGGGGACTGTGAGATGGAGGACCTGATGCCCTGCTCCCTAGGCACGTTTGTC CTGGTGCAGTGA Clone variation with respect to NM_173354.3 167 a=>t;168 a=>g;1785 g=>t >OriGene 5' read for mutant NM_173354 unedited
ACGCCGTTTGAGCAATGGGCGGTAGGCGCTGTACGGCTGTGGAGGTCTATATAAGCAGAGCTCATTTAGG CTGACACTATAGAATACAAGCTACTATGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCGGAGGC AGCAGCAGCGGCGGCGGCAGCCGGAGCAGTAGGCACCCGAGCAGCGCCAGCGGCCGAGCGGGCGGCTTCC TGGCCTGGGCGCTCCGGTGGCGGCGGAGGTGCGCGCGGAGCCATGGTTATCATGTCGGAGTTCAGCGCGG ACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAGCCCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCT GGGCAAAGGCAACTTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAATG ATAATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGC TTCTGAACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATGCTTTACATCGTCAC TGATTTGCTAAAAATGGAGAAATGTTTGATTATTTGACTTCCACGGGCACTGAGTGAGACCGAGGCGCGA GAGTTCTGGCAAATCCTTGTCGGCCGGTGGGAGTACTGTCACCGACATCCACATCGTCCACCGGGACCTC AGAACCGAAGACCTCTGCTGAATGCAACATGAACTCAGGCTGCGATTTTGAATTTGGAATTTCTACAGTC AGGAAAGCCCTCTGTCCTTTGGGTGGGGGAGCCCCCGTATGCCGCCCGGGAGTCTTTGAGGGAAGAGATT AGAAGGCCCCACCTGAAACTCTGGAACCTGGCGTGTGTGCTGACGTCTGATCACGTCTCCCTCGCATGGC TACTGGCAGCTGAACCGGTCTCTGAGCCATTCGCCATCGA >SC323562 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTA TAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCGGAGGCAGCAGCAGCG GCGGCGGCAGCCGGAGCAGTAGGCACCCGAGCAGCGCCAGCGGCCGAGCGGGCGGCTTCCTGGCCTGGGC GCTCCGGTGGCGGCGGAGGTGCGCGCGGAGCCATGGTTATCATGT |
| Restriction Sites | Please inquire |
| ACCN | NM_173354 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173354.2, NP_775490.1 |
| RefSeq Size | 4723 bp |
| RefSeq ORF | 2352 bp |
| Locus ID | 150094 |
| UniProt ID | P57059 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/threonine protein kinase that contains a ubiquitin-associated (UBA) domain. The encoded protein is a member of the adenosine monophosphate-activated kinase (AMPK) subfamily of kinases that play a role in conserved signal transduction pathways. A mutation in this gene is associated with early infantile epileptic encephalopathy 30. [provided by RefSeq, Nov 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206169 | SIK1 (Myc-DDK-tagged)-Human salt-inducible kinase 1 (SIK1) |
CNY 5,736.00 |
|
| RC206169L1 | Lenti ORF clone of Human salt-inducible kinase 1 (SIK1), Myc-DDK-tagged |
CNY 8,136.00 |
|
| RC206169L2 | Lenti ORF clone of Human salt-inducible kinase 1 (SIK1), mGFP tagged |
CNY 5,890.00 |
|
| RC206169L3 | Lenti ORF clone of Human salt-inducible kinase 1 (SIK1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206169L4 | Lenti ORF clone of Human salt-inducible kinase 1 (SIK1), mGFP tagged |
CNY 5,890.00 |
|
| RG206169 | SIK1 (tGFP-tagged) - Human salt-inducible kinase 1 (SIK1) |
CNY 7,336.00 |
|
| SC101272 | SIK1 (untagged)-Human salt-inducible kinase 1 (SIK1) |
CNY 5,744.00 |
