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PKC nu (PRKD3) (NM_005813) Human Untagged Clone
CAT＃: SC323561
PRKD3 (untagged)-Kinase deficient mutant (K605M) of Human protein kinase D3 (PRKD3)
CNY 6,528.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EPK2; nPKC-NU; PKC-NU; PKD3; PRKCN |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323561 sequence for NM_005813 edited (data generated by NextGen Sequencing)
ATGTCTGCAAATAATTCCCCTCCATCAGCCCAGAAGTCTGTATTACCCACAGCTATTCCT GCTGTGCTTCCAGCTGCTTCTCCGTGTTCAAGTCCTAAGACGGGACTCTCTGCCCGACTC TCTAATGGAAGCTTCAGTGCACCATCACTCACCAACTCCAGAGGCTCAGTGCATACAGTT TCATTTCTACTGCAAATTGGCCTCACACGGGAGAGTGTTACCATTGAAGCCCAGGAACTG TCTTTATCTGCTGTCAAGGATCTTGTGTGCTCCATAGTTTATCAAAAGTTTCCAGAGTGT GGATTCTTTGGCATGTATGACAAAATTCTTCTCTTTCGCCATGACATGAACTCAGAAAAC ATTTTGCAGCTGATTACCTCAGCAGATGAAATACATGAAGGAGACCTAGTGGAAGTGGTT CTTTCAGCTTTAGCCACAGTAGAAGACTTCCAGATTCGTCCACATACTCTCTATGTACAT TCTTACAAAGCTCCTACTTTCTGTGATTACTGTGGTGAGATGCTCTGGGGATTGGTACGT CAAGGACTGAAATGTGAAGGCTGTGGATTAAATTACCATAAACGATGTGCCTTCAAGATT CCAAATAACTGTAGTGGAGTAAGAAAGAGACGTCTGTCAAATGTATCTTTACCAGGACCC GGCCTCTCAGTTCCAAGACCCCTACAGCCTGAATATGTAGCCCTTCCCAGTGAAGAGTCA CATGTCCACCAGGAACCAAGTAAGAGAATTCCTTCTTGGAGTGGTCGCCCAATCTGGATG GAAAAGATGGTAATGTGCAGAGTGAAAGTTCCACACACATTTGCTGTTCACTCTTACACC CGTCCCACGATATGTCAGTACTGCAAGCGGTTACTGAAAGGCCTCTTTCGCCAAGGAATG CAGTGTAAAGATTGCAAATTCAACTGCCATAAATGCTGTGCATCAAAAGTACCAAGAGAC TGCCTTGGAGAGGTTACTTTCAATGGAGAACCTTCCAGTCTGGGAACAGATACAGATATA CCAATGGATATTGACAATAATGACATAAATAGTGATAGTAGTCGGGGTTTGGATGACACA GAAGAGCCATCACCCCCAGAAGATAAGATGTTCTTCTTGGACCCATCTGATCTCGATGTG GAAAGAGATGAAGAAGCCGTTAAAACAATCAGTCCATCAACAAGCAATAATATTCCGCTC ATGAGGGTTGTACAATCCATCAAGCACACAAAGAGGAAGAGCAGCACAATGGTGAAGGAA GGGTGGATGGTCCATTACACCAGCAGGGATAACCTGAGAAAGAGGCATTATTGGAGACTT GACAGCAAATGTCTAACATTATTTCAGAATGAATCTGGATCAAAGTATTATAAGGAAATT CCACTTTCAGAAATTCTCCGCATATCTTCACCACGAGATTTCACAAACATTTCACAAGGC AGCAATCCACACTGTTTTGAAATCATTACTGATACTATGGTATACTTCGTTGGTGAGAAC AATGGGGACAGCTCTCATAATCCTGTTCTTGCTGCCACTGGAGTTGGACTTGATGTAGCA CAGAGCTGGGAAAAAGCAATTCGCCAAGCCCTCATGCCTGTTACTCCTCAAGCAAGTGTT TGCACTTCTCCAGGGCAAGGGAAAGATCACAAAGATTTGTCTACAAGTATCTCTGTATCT AATTGTCAGATTCAGGAGAATGTGGATATCAGTACTGTTTACCAGATCTTTGCAGATGAG GTGCTTGGTTCAGGCCAGTTTGGCATCGTTTATGGAGGAAAACATAGAAAGACTGGGAGG GATGTGGCTATTAAAGTAATTGATAAGATGAGATTCCCCACAAAACAAGAAAGTCAACTC CGTAATGAAGTGGCTATTTTACAGAATTTGCACCATCCTGGGATTGTAAACCTGGAATGT ATGTTTGAAACCCCAGAACGAGTCTTTGTAGTAATGGAAAAGCTGCATGGAGATATGTTG GAAATGATTCTATCCAGTGAGAAAAGTCGGCTTCCAGAACGAATTACTAAATTCATGGTC ACACAGATACTTGTTGCTTTGAGGAATCTGCATTTTAAGAATATTGTGCACTGTGATTTA AAGCCAGAAAATGTGCTGCTTGCATCAGCAGAGCCATTTCCTCAGGTGAAGCTGTGTGAC TTTGGATTTGCACGCATCATTGGTGAAAAGTCATTCAGGAGATCTGTGGTAGGAACTCCA GCATACTTAGCCCCTGAAGTTCTCCGGAGCAAAGGTTACAACCGTTCCCTAGATATGTGG TCAGTGGGAGTTATCATCTATGTGAGCCTCAGTGGCACATTTCCTTTTAATGAGGATGAA GATATAAATGACCAAATCCAAAATGCTGCATTTATGTACCCACCAAATCCATGGAGAGAA ATTTCTGGTGAAGCAATTGATCTGATAAACAATCTGCTTCAAGTGAAGATGAGAAAACGT TACAGTGTTGACAAATCTCTTAGTCATCCCTGGCTACAGGACTATCAGACTTGGCTTGAC CTTAGAGAATTTGAAACTCGCATTGGAGAACGTTACATTACACATGAAAGTGATGATGCT CGCTGGGAAATACATGCATACACACATAACCTTGTATACCCAAAGCACTTCATTATGGCT CCTAATCCAGATGATATGGAAGAAGATCCTTAA Clone variation with respect to NM_005813.3 934 c=>t;1122 t=>c;1200 a=>c >OriGene 5' read for mutant NM_005813 unedited
ACCGACCGTTGAAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTTTTATTCAATA TGTATTTCCTGAAATTAAAGAGACAAGTACAGACTGAAAGGAAAATAGATTCGTAAATAAGCTACGTCAA CTCTATCCTGCTGAGGATAGCTCAGTGATGTTAAATCCTTTACAAATCCCTGGTTGTCTTCCTACAGACA AGACTGCTTTTTGATGGGACTGATATTAAGAGAAATAGGACCTTTGGGGCATTCAACTCCTTGATAAAAC TTAAAAGTATCGGCATGAGTGGCTTAACAGAGGAAATAAAGAAGTTTTCAACTAAATCCAAAAGTGCGGT CATTTTCTTTACTGCTGTTATTTTAAAAACCTCTTCATAACCATTGAAAAAGATCGACAACTATTTTAAA AGATTAAAGAAAGGCAGATGTCTGCAAATAATTCCCCTCCATCAGCCCAGAGTCTGTATTACCCACAGCT ATTCCTGCTGTGCTTCCAGCTGCTTCTCGTGTCAAGTCCTAGACGGACCTCTCTGCCGACTCTCTAATGA AGCTCAGTGGCACATCCCTCACAACTCAGAAGCTCATGCATACAGTTTCATTTCTACTGCAAATTGGCCT ACACACGGGAGAGTTGTTAACATTGAGCCAAGGAACCTGTCCTTTATCCTGCGTGTCAGGATTCTTGTGT GCCTCTATGTTATACAAAGGTTCCCGAGGTGGGATTCTTGCCATGTTGCCAATTTTTCTTTGCCTGACAT GAATCTTGAAAACTTGACCTGTGATATACCTAGCGTGATCCTGAGAGAACCTTGAATGTTGCTAGCTTGC CAGTGAAAACTCGAATTGCCCACTCTTAGACCTCAGCGGCTCAATG >SC323561 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CWCAGTMTGTTTACMGATCTTTGCAGATGAGGTGCTTGGTTCAGGCCAGTTTGGCATCGTTTATGGAGGA AAACATAGAAAGACTGGGAGGGATGTGGCTATTATGGTAATTGATAAGATGAGATTCCCCACAAAACAAG AAAGTCAACTCCGTAATGAAGTGGCTATTTTACAGAATTTGCACCATCCTGGGATTGTAAACCTGGAATG TATGTTTGAAACCCCAGAACGAGTCTTTGTAGTAATGGAAAAGCT |
| Restriction Sites | Please inquire |
| ACCN | NM_005813 |
| Insert Size | 6220 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005813.3, NP_005804.1 |
| RefSeq Size | 5907 bp |
| RefSeq ORF | 2673 bp |
| Locus ID | 23683 |
| UniProt ID | O94806 |
| Domains | pkinase, TyrKc, PH, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221309 | PRKD3 (Myc-DDK-tagged)-Human protein kinase D3 (PRKD3) |
CNY 7,130.00 |
|
| RC221309L1 | Lenti ORF clone of Human protein kinase D3 (PRKD3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221309L2 | Lenti ORF clone of Human protein kinase D3 (PRKD3), mGFP tagged |
CNY 9,030.00 |
|
| RC221309L3 | Lenti ORF clone of Human protein kinase D3 (PRKD3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221309L4 | Lenti ORF clone of Human protein kinase D3 (PRKD3), mGFP tagged |
CNY 9,030.00 |
|
| RG221309 | PRKD3 (tGFP-tagged) - Human protein kinase D3 (PRKD3) |
CNY 8,120.00 |
|
| SC116506 | PRKD3 (untagged)-Human protein kinase D3 (PRKD3) |
CNY 6,528.00 |
