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DYRK4 (NM_003845) Human Untagged Clone
CAT＃: SC323557
DYRK4 (untagged)-Kinase deficient mutant (K133M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4)
CNY 3,880.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003845, the custom clone sequence may differ by one or more nucleotides
ATGCCGGCCTCAGAGCTCAAGGCTTCAGAAATACCTTTCCACCCTAGCATTAAAACCCAGGATCCCAAGG CAGAGGAGAAGTCACCAAAGAAGCAAAAGGTGACTCTGACAGCGGCAGAGGCCCTAAAGCTTTTTAAGAA CCAGCTGTCTCCATATGAACAAAGTGAAATCCTGGGCTACGCGGAGCTGTGGTTCCTGGGTCTTGAAGCC AAGAAGCTCGACACGGCTCCTGAGAAATTTAGCAAGACGAGTTTTGATGATGAGCATGGCTTCTATCTGA AGGTCCTGCATGATCACATTGCCTACCGCTATGAAGTTCTGGAGACAATCGGGAAGGGGTCCTTTGGACA GGTGGCCAAGTGCTTGGATCACAAAAACAATGAGCTGGTGGCCCTGAAAATCATCAGGAACAAGAAGAGG TTTCACCAGCAGGCCCTGATGGAGCTGAAGATCCTGGAAGCTCTCAGAAAGAAGGACAAAGACAACACCT ACAATGTGGTGCATATGAAGGACTTTTTCTACTTTCGCAATCACTTCTGCATCACCTTTGAGCTCCTGGG AATCAACTTGTATGAGTTGATGAAGAATAACAACTTTCAAGGCTTCAGTCTGTCCATAGTTCGGCGCTTC ACTCTCTCTGTTTTGAAGTGCTTGCAGATGCTTTCGGTAGAGAAAATCATTCACTGTGATCTCAAGCCCG AAAATATAGTGCTATACCAAAAGGGCCAAGCCTCTGTTAAAGTCATTGACTTTGGATCAAGCTGTTATGA ACACCAGAAAGTATACACGTACATCCAAAGCCGGTTCTACCGATCCCCAGAAGTGATCCTGGGCCACCCC TACGACGTGGCCATTGACATGTGGAGCCTGGGCTGCATCACGGCGGAGTTGTACACGGGCTACCCCCTGT TCCCCGGGGAGAATGAGGTGGAGCAGCTGGCCTGCATCATGGAGGTGCTGGGTCTGCCGCCAGCCGGCTT CATTCAGACAGCCTCCAGGAGACAGACATTCTTTGATTCCAAAGGTTTTCCTAAAAATATAACCAACAAC AGGGGGAAAAAAAGATACCCAGATTCCAAGGACCTCACGATGGTGCTGAAAACCTATGACACCAGCTTCC TGGACTTTCTCAGAAGGTGTTTGGTATGGGAACCTTCTCTTCGCATGACCCCGGACCAGGCCCTCAAGCA TGCTTGGATTCATCAGTCTCGGAACCTCAAGCCACAGCCCAGGCCCCAGACCCTGAGGAAATCCAATTCC TTTTTCCCCTCTGAGACAAGGAAGGACAAGGTTCAAGGCTGTCATCACTCGAGCAGAAAAGCAGATGAGA TCACCAAAGAGACTACAGAGAAAACAAAAGATAGCCCCACGAAGCATGTTCAGCATTCAGGTGATCAGCA GGACTGTCTCCAGCACGGAGCTGACACTGTTCAGCTGCCTCAACTGGTAGACGCTCCCAAGAAGTCAGAG GCAGCTGTCGGGGCGGAGGTGTCCATGACCTCCCCAGGACAGAGCAAAAACTTCTCCCTCAAGAACACAA ACGTTTTACCCCCTATTGTATGA >OriGene 5' read for mutant NM_003845 unedited
CCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAA GCTTATGGATTCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGCGTGAGCTGTTGAAAGCCT GCAGCTAAACACCAGTGTTACTTCACTCCCCTTTGTGGACACCAAGGGGAAGAAGAATACGGTAAGCTTC CCACACATTAGCAAGAAAGTCCTGCTGAAGTCATCCCCTGCTGTATCAGGAGAATCAAGCTCACAATCAG ATGCCCGGCCTCAGAGCTCAAGGCTTCAGAAAATACCTTTCCACCCTAGCATTAAAACCCCAGGATCCCC AGGCAGAAGGAAAAGTCACCAAAGAGCCAAAGTTGACTCTGACACCGGCGAAGGCCCTAAAGCTTTTAAG AACCAGCTGTTTCCTTATGACAAAGTTGAATCCTGGGGCTACCCGGAGCTGTGGGTCCTGGGGCCTGGAC CAAGAAGTCGAACCGGTCTCGGAAAATTTACCAGAGCAGTTTGAGAGGAGACCTTGTTTCTACTGAAAGG TTCCATGGATCAATGCCCTACGATTGAAGTCCTAGACACTCCGGAAGGGTCCTTGACCGTGTCGCAAGCT CTGTTCACACACTATGCCGTGTGCCTTGATATACGAGACAAGAGTTACCCGCGCGTGAGCTAGATATCGG ACCCCAGAGACACAACCCATGGTGCTATAGAGATTCTCTCCCCTTGCCCCTGTCCGGACCTGTATAGAGA AACCTAGCGCTCGCATTTGCACTCTCGGTGATGCCGCACGGGGAAACCGGGGGG >SC323557 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMKATCATTGCCTACCGCTATGAGTTCTGGAGACAATCGGGAAGGGGTCCTTTGGACAGGTGGCCAAGTG CTTGGATCACAAAAACAATGAGCTGGTGGCCCTGATGATCATCAGGAACAAGAAGAGGTTTCACCAGCAG GCCCTGATGGAGCTGAAGATCCTGGAAGCTCTCAGAAAGAAGGACAAAGACAACACCTACAATGTGGTGC ATATGAAGGACTTTTTCTACTTTCGCAATCACTTCTGCATCACCT |
| Restriction Sites | Please inquire |
| ACCN | NM_003845 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003845.1, NP_003836.1 |
| RefSeq Size | 1860 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 8798 |
| UniProt ID | Q9NR20 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206929 | DYRK4 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) |
CNY 3,872.00 |
|
| RC206929L1 | Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), Myc-DDK-tagged |
CNY 6,272.00 |
|
| RC206929L2 | Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), mGFP tagged |
CNY 5,890.00 |
|
| RC206929L3 | Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206929L4 | Lenti ORF clone of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), mGFP tagged |
CNY 5,890.00 |
|
| RG206929 | DYRK4 (tGFP-tagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) |
CNY 5,472.00 |
|
| SC106952 | DYRK4 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) |
CNY 3,880.00 |
