MEK5 (MAP2K5) (NM_145161) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dual specificity mitogen-activated protein kinase kinase 5; HsT17454; MAPK/ERK kinase 5; MAP kinase kinase MEK5b; MAPKK5; MEK5; MEK5, MAPKK5, PRKMK5, HsT17454; mitogen-activated protein kinase kinase 5; PRKMK5
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_145161, the custom clone sequence may differ by one or more nucleotides ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCC CAAATAGTGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGT GATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATT ACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTT CCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAAC AAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCC GAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGA ACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTA CATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGTGATTTTGGAG TTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGATTCAG AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAG GCCAGCACCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTG TCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCTGGCTTCCCTGCCCTCGCCTTCACCTCTGT CAGCAGGTGGCCTTGCCTGGGGAGCCCCATGTGTGGCCCACCCCACCAGGCCATCCCCATACCTTCTGGT TTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant NM_145161 unedited ACGCCCGTCTCAGCAATGGGCGGTAGGCGCTGTACGGTGGGAGGATCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGACCTTCAC CATAGCGTTCGCTCAACTCCAGAACCTTCCGACCTCCGCTAGTTCCTGCGGGCCTTTGCCCGCTTCCCGG TGCACCCTCCCCGGGAGACACCTCAGACCCCCGACAGCCTGGGCAGGCTCGGTGCCTGCGGGTGCGTTCC TGATCACCCCTCCCCTCTTCCCTCCCCCCTCATCCCTCCATTCCCCTTGTTTTTCACCCCTCTGTCCTCT GCCCGTCACTCCCCCTTGGTCACCTCTTTGGAGCCCCCCTTCCTACCCAGCGGCAAGTGGGGTTTTCCCA TACCCCCAGAAGGTGGAGCCTTCTTTTAACCTGGTATTGCTGGTGGCTTAGCCCTGGGCCCCTTTCTGGC ATTGGGAAAAACAAGTTGCTGTTATTTGGCATCAGGATCCCAATAGTGGGCCGTTGAACTGACCGTTGCA TTCCGGCCGCGAGTATTCTCTTCAGGGAGTGCTGGATGTGTGATAGCCCAGTTTTTGCCGAAACACACCT CAACCGCATTTAAATATAGATAAGATAGTGATATCATTTACTGTGAATGTGAGAGATGAGGGCAGTGTGT CATATATTCTCCACTATGTGCACACGTTAGTGACTTATATAACTCTCTGATATTCCAGACGCGACCCTCG GAAGACAACATAGCGCAGGAGTATCGCGCGATCTTAACAGCACAGCTGGTATCTACGACTATAAACTCTC TGTACGAAAATCACTCGCGCGTATGTACCACCATTGAACCTGATGACGCAGACCTACAACATGCTGGGAT TACTGGTGACACACAT Kinase domain sequence (showhide) >SC323547 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation GCAGATGATGACAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCA TATCATGTCCCGAGTGGGAAAATATTAGCTGTAATGGTCATACTACTAGATATTACACTGGAACTTCAGA AGCAAATTATGTCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGC ATTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCGGCAC
Restriction Sites	Please inquire
ACCN	NM_145161
Insert Size	5500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_145161.1, NP_660144.1
RefSeq Size	2229 bp
RefSeq ORF	2229 bp
Locus ID	5607
Domains	PB1, pkinase, TyrKc, S_TKc
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway
Gene Summary	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011] Transcript Variant: This variant (C) encodes the longest isform (C).

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