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TAOK3 (NM_016281) Human Untagged Clone
CAT＃: SC323536
TAOK3 (untagged)-Kinase deficient mutant (K53M) of Human TAO kinase 3 (TAOK3)
CNY 6,584.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DPK; hKFC-A; JIK; MAP3K18 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323536 sequence for NM_016281 edited (data generated by NextGen Sequencing)
ATGCGTAAAGGGGTGCTGAAGGACACAGAGATTGCCGATCTATTCTACAAAGATGATCCT GAGGAACTTTTTATTGGTTTGCATGAAATTGGACATGGAAGTTTTGGAGCAGTTTATTTT GCTACAAATGCTCACACCAATGAGGTGGTGGCAATTATGAAGATGTCCTATAGTGGGAAG CAGACCCATGAGAAATGGCAAGATATTCTTAAGGAAGTTAAATTTTTACGACAATTGAAG CATCCTAATACTATTGAGTACAAAGGCTGTTACTTGAAAGAACACACTGCTTGGTTGGTG ATGGAATATTGCTTAGGCTAAGCCTCTGATTTATTAGAAGTTCATAAAAAACCACTTCAG GAAGTGGAGATCGCTGCCATTACTCATGGAGCCTTGCATGGACTAGCCTACCTACATTCT CATGCATTGATTCATAGGGATATTAAAGCAGGAAATATTCTTCTAACAGAGCCAGGTCAG GTAAAACTAGCTGATTTTGGATCTGCTTCAATGGCTTCTCCTGCCAACTCCTTCGTGGGC ACACCTTACTGGATGGCTCCAGAGGTGATCTTAGCTATGGATGAAGGACAGTATGATGGG AAAGTTGATATTTGGTCACTTGGCATCACTTGTATTGAATTGGCGGAACGGAAGCCGCCC CTTTTCAACATGAATGCAATGAGTGCCTTATATCACATTGCCCAGAATGACTCCCCAACG TTACAGTCTAATGAATGGACAGACTCCTTTAGGAGATTTGTTGATTACTGCTTGCAGAAA ATACCTCAGGAAAGGCCAACATCAGCAGAACTATTAAGGCATGACTTTGTTCGACGAGAC CGGCCACTACGTGTCCTCATTGACCTCATACAGAGGACAAAAGATGCAGTTCGTGAGCTA GATAACCTACAGTACCGAAAAATGAAAAAAATACTTTTCCAAGAGACACGGAATGGACCC TTGAATGAGTCACAGGAGGATGAGGAAGACAGTGAACATGGAACCAGCCTGAACAGGGAA ATGGACAGCCTGGGCAGCAACCATTCCATTCCAAGCATGTCCGTGAGCACAGGCAGCCAG AGCAGCAGTGTGAACAGCATGCAGGAAGTCATGGACGAGAGCAGTTCCGAACTTGTCATG ATGCACGATGACGAAAGCACAATCAATTCCAGCTCCTCCGTCGTGCATAAGAAAGATCAT GTATTCATAAGGGATGAGGCGGGCCACGGCAATCCCAGGCCTGAGCCGCGGCCTACCCAG TCAGTTCAGAGCCAGGCCCTCCACTACCGGAACAGAGAGCGCTTTGCCACGATCAAATCA GCATCTTTGGTTACACGACAGATCCATGAGCATGAGCAGGAGAACGAGTTGCGGGAACAG ATGTCAGGTTATAAGCGGATGCGGCGCCAGCACCAGAAGCAGCTGATCGCCCTGGAGAAC AAGCTGAAGGCTGAGATGGACGAGCACCGCCTCAAGCTACAGAAGGAGGTGGAGACGCAT GCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGAAGCAAGTGGCTATCATAGAA AAGGAGGCAAAGGTAGCTGCAGCAGATGAGAAGAAGTTCCAGCAACAGATCTTGGCCCAG CAGAAGAAAGATTTGACAACTTTCTTAGAAAGTCAGAAGAAGCAGTATAAGATTTGTAAG GAAAAAATAAAAGAGGAAATGAATGAGGACCATAGCACACCCAAGAAAGAGAAGCAAGAG CGGATCTCCAAACATAAAGAGAACTTGCAGCACACACAGGCTGAAGAGGAAGCCCACCTT CTCACTCAACAGAGACTGTACTACGACAAAAATTGTCGTTTCTTCAAGCGGAAAATAATG ATCAAGCGGCACGAGGTGGAGCAGCAGAACATTCGGGAGGAACTAAATAAAAAGAGGACC CAGAAGGAGATGGAGCATGCCATGCTAATCCGGCACGACGAGTCCACCCGAGAGCTAGAG TACAGGCAGCTGCACACGTTACAGAAGCTACGCATGGATCTGATCCGTTTACAGCACCAG ACGGAACTGGAAAACCAGCTGGAGTACAATAAGAGGCGAGAAAGAGAACTGCACAGAAAG CATGTCATGGAACTTCGGCAACAGCCAAAAAACTTAAAGGCCATGGAAATGCAAATTAAA AAACAGTTTCAGGACACTTGCAAAGTACAGACCAAACAGTATAAAGCACTCAAGAATCAC CAGTTGGAAGTTACTCCAAAGAATGAGCACAAAACAATCTTAAAGACACTGAAAGATGAG CAGACAAGAAAACTTGCCATTTTGGCAGAGCAGTATGAACAGAGTATAAATGAAATGATG GCCTCTCAAGCGTTACGGCTAGATGAGGCTCAAGAAGCAGAATGCCAGGCCTTGAGGCTA CAGCTCCAGCAGGAAATGGAGCTGCTCAACGCCTACCAGAGCAAAATCAAGATGCAAACA GAGGCACAACATGAACGTGAGCTCCAGAAGCTAGAGCAGAGAGTGTCTCTGCGCAGAGCA CACCTTGAGCAGAAGATTGAAGAGGAGCTGGCTGCCCTTCAGAAGGAACGCAGCGAGAGA ATAAAGAACCTATTGGAAAGGCAAGAGCGAGAGATTGAAACTTTTGACATGGAGAGCCTC AGAATGGGATTTGGGAATTTGGTTACATTAGATTTTCCTAAGGAGGACTACAGATGA Clone variation with respect to NM_016281.3 25 c=>a;140 g=>a;158 a=>t;320 c=>a;1231 g=>a >OriGene 5' read for mutant NM_016281 unedited
CCCGCCCGTCTCAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTTCTATATAAGCAGAGCTCGTTTA GTGAACCGTCAGAATCCTGTAATACGACTCACTATAGGGCGGCCGCGAATTTCGGCACGAGGCAAAAAGC AGAGCCTGCTCTACTGGCCATCATGCGTAAAGGGGCGCTGAAGGACACAGAGATTGCCGATTCTATTCTA CAAAGATGACTCCTGAGGAACTTTTTATTGGTTTGCATGAAATTGGACATGGAAGCTTTGGAGCAGTTTA TTTTGCTACAAATGCTCACACCAATGAGGTGGTGGCAATTATGAAGATGTCCTATAGTGGGGAAGCAGAC CCATGAGAAAATGGCAAGATATTTCTTTAAGGAAGTTTAAATTTTTTACGACAATTTGAAGCATCCCTAA TACTATTTGAGTTACAAAGGCTGTTACTTTGAAAGAACAACACTGCTTTGTTTGGTTGATTGAATTATTG CTAAGGCCTAACCCTTCTGATTTATTTAGAAGTTCCATAAAAAAACCACTTCCAGGAGTTGGAGATTGCC TGGCCATTAACTAATGGGACCCTTGCATGACCTAGCTCACCTCCCTTTCTATTGCATTTGATCATTAGGG ATTATAAGCCAAGGAATATTTTTTTCAAACGAAGCCAGTTCAGGTAAAACTGCTGGATTTGGATCTCTGC TTATAGGGTCTTCTGGCCATCTCTTGCGTGGACACACTATACGGGTGTGTCTCGAGGTGTGATCTATCTG TGAAGAGACTTGATGTGGAATGTATTGGCACCTGGACACATGGATGAATGCGGACGAGAACGCGCCTTTC ACGATGCCGAAGTGCTCTATACACATGCGCAATGATTCCCAGTCAGCGTAGTTGGCACTCTTAGAATTGT GTACTGTGCAACCTGAGGACATCGACATTACGACTTGCGACGCACTAGTCTAACC >SC323536 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CRAWGWCTGAGGACTTTTTATTGGTTTGCATGAATTGGACATGGAAGTTTTGGAGCAGTTTATTTTGCTA CAAATGCTCACACCAATGAGGTGGTGGCAATTATGAAGATGTCCTATAGTGGGAAGCAGACCCATGAGAA ATGGCAAGATATTCTTAAGGAAGTTAAATTTTTACGACAATTGAAGCATCCTAATACTATTGAGTACAAA GGCTGTTACTTGAAAGAACACACTGCTTGGTTGGTGATGGAATAT |
| Restriction Sites | Please inquire |
| ACCN | NM_016281 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016281.2, NP_057365.2 |
| RefSeq Size | 4188 bp |
| RefSeq ORF | 2697 bp |
| Locus ID | 51347 |
| UniProt ID | Q9H2K8 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (2), as well as variants 3 and 4, encodes isoform b. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201031 | TAOK3 (Myc-DDK-tagged)-Human TAO kinase 3 (TAOK3) |
CNY 6,576.00 |
|
| RC201031L1 | Lenti ORF clone of Human TAO kinase 3 (TAOK3), Myc-DDK-tagged |
CNY 8,976.00 |
|
| RC201031L2 | Lenti ORF clone of Human TAO kinase 3 (TAOK3), mGFP tagged |
CNY 9,030.00 |
|
| RC201031L3 | Lenti ORF clone of Human TAO kinase 3 (TAOK3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC201031L4 | Lenti ORF clone of Human TAO kinase 3 (TAOK3), mGFP tagged |
CNY 9,030.00 |
|
| RG201031 | TAOK3 (tGFP-tagged) - Human TAO kinase 3 (TAOK3) |
CNY 7,890.00 |
|
| SC114377 | TAOK3 (untagged)-Human TAO kinase 3 (TAOK3) |
CNY 2,950.00 |
