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IRE1 (ERN2) (NM_033266) Human Untagged Clone
CAT＃: SC323535
ERN2 (untagged)-Kinase deficient mutant (K596M) of Human endoplasmic reticulum to nucleus signaling 2 (ERN2)
CNY 7,144.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hIRE2p; IRE1-BETA; IRE1b; IRE2p |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_033266, the custom clone sequence may differ by one or more nucleotides
ATGAGAAGGGCGGGGATCGGCGAGGACTCCAGGCTGGGGTTGCAGGCCCAGCCAGGGGCGGAGCCTTCTC CGGGTCGGGCGGGGACAGAGCGCTCCCTTGGAGGCACCCAGGGACCTGGCCAGCCGTGCAGCTGCCCAGG CGCTATGGCGAGTGCGGTCAGGGGGTCGAGGCCGTGGCCCCGGCTGGGGCTCCAGCTCCAGTTCGCGGCG CTGCTGCTCGGGACGCTGAGTCCACAGGTTCATACTCTCAGGCCAGAGAACCTCCTGCTGGTGTCCACCT TGGATGGAAGTCTCCACGCACTAAGCAAGCAGACAGGGGACCTGAAGTGGACTCTGAGGGATGATCCCGT CATCGAAGGACCAATGTACGTCACAGAAATGGCCTTTCTCTCTGACCCAGCAGATGGCAGCCTGTACATC TTGGGGACCCAAAAACAACAGGGATTAATGAAACTGCCATTCACCATCCCTGAGCTGGTTCATGCCTCTC CCTGCCGCAGCTCTGATGGGGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTGGTGGACCCTGA GTCAGGGGAGACCCAGATGACACTGACCACAGAGGGTCCCTCCACCCCCCGCCTCTACATTGGCCGAACA CAGTATACGGTCACCATGCATGACCCAAGAGCCCCAGCCCTGCGCTGGAACACCACCTACCGCCGCTACT CAGCGCCCCCCATGGATGGCTCACCTGGGAAATACATGAGCCACCTGGCGTCCTGCGGGATGGGCCTGCT GCTCACTGTGGACCCAGGAAGCGGGACGGTGCTGTGGACACAGGACCTGGGCGTGCCTGTGATGGGCGTC TACACCTGGCACCAGGACGGCCTGCGCCAGCTGCCGCATCTCACGCTGGCTCGAGACACTCTGCATTTCC TCGCCCTCCGCTGGGGCCACATCCGACTGCCTGCCTCAGGCCCCCGGGACACAGCCACCCTCTTCTCTAC CTTGGACACCCAGCTGCTAATGACGCTGTATGTGGGGAAGGATGAAACTGGCTTCTATGTCTCTAAAGCA CTGGTCCACACAGGAGTGGCCCTGGTGCCTCGTGGACTGACCCTGGCCCCCGCAGATGGCCCCACCACAG ATGAGGTGACACTCCAAGTCTCAGGAGAGCGAGAGGGCTCACCCAGCACTGCTGTTAGATACCCCTCAGG CAGTGTGGCCCTCCCAAGCCAGTGGCTGCTCATTGGACACCACGAGCTACCCCCAGTCCTGCACACCACC ATGCTGAGGGTCCATCCCACCCTGGGGAGTGGAACTGCAGAGACAAGACCTCCAGAGAATACCCAGGCCC CAGCCTTCTTCTTGGAGCTATTGAGCCTGAGCCGAGAGAAACTTTGGGACTCCGAGCTGCATCCAGAAGA AAAAACTCCAGACTCTTACTTGGGGCTGGGACCCCAAGACCTGCTGGCAGCTAGCCTCACTGCTGTCCTC CTGGGAGGGTGGATTCTCTTTGTGATGAGGCAGCAACAGCCGCAGGTGGTGGAGAAGCAGCAGGAGACCC CCCTGGCACCTGCAGACTTTGCTCACATCTCCCAGGATGCCCAGTCCCTGCACTCGGGGGCCAGCCGGAG GAGCCAGAAGAGGCTTCAGAGTCCCTCAAAGCAAGCCCAGCCACTCGACGACCCTGAAGCTGAGCAACTC ACCGTAGTGGGGAAGATTTCCTTCAATCCCAAGGACGTGCTGGGCCGCGGGGCAGGCGGGACTTTCGTTT TCCGGGGACAGTTTGAGGGACGGGCAGTGGCTGTCAAGCGGCTCCTCCGCGAGTGCTTTGGCCTGGTTCG GCGGGAAGTTCAACTGCTGCAGGAGTCTGACAGGCACCCCAACGTGCTCCGCTACTTCTGCACCGAGCGG GGACCCCAGTTCCACTACATTGCCCTGGAGCTCTGCCGGGCCTCCTTGCAGGAGTACGTAGAAAACCCGG ACCTGGATCGCGGGGGTCTGGAGCCCGAGGTCGTGCTGCAGCAGCTGATGTCTGGCCTGGCCCACCTGCA CTCTTTACACATAGTGCACCGGGACCTGAAGCCAGGAAATATTCTCATCACCGGGCCTGACAGCCAGGGC CTGGGCAGAGTGGTGCTCTCAGACTTCGGCCTCTGCAAGAAGCTGCCTGCTGGCCGCTGTAGCTTCAGCC TCCACTCCGGCATCCCCGGCACGGAAGGCTGGATGGCGCCCGAGCTTCTGCAGCTCCTGCCACCAGACAG TCCTACCAGCGCTGTGGACATCTTCTCTGCAGGCTGCGTGTTCTACTACGTGCTTTCTGGTGGCAGCCAC CCCTTTGGAGACAGTCTTTATCGCCAGGCAAACATCCTCACAGGGGCTCCCTGTCTGGCTCACCTGGAGG AAGAGGTCCACGACAAGGTGGTTGCCCGGGACCTGGTTGGAGCCATGTTGAGCCCACTGCCGCAGCCACG CCCCTCTGCCCCCCAGGTGCTGGCCCACCCCTTCTTTTGGAGCAGAGCCAAGCAACTCCAGTTCTTCCAG GACGTCAGTGACTGGCTGGAGAAGGAGTCCGAGCAGGAGCCCCTGGTGAGGGCACTGGAGGCGGGAGGCT GCGCAGTGGTCCGGGACAACTGGCACGAGCACATCTCCATGCCGCTGCAGACAGATCTGAGAAAGTTCCG GTCCTATAAGGGGACATCAGTGCGAGACCTGCTCCGTGCTGTGAGGAACAAGAAGCACCACTACAGGGAG CTCCCAGTTGAGGTGCGACAGGCACTCGGCCAAGTCCCTGATGGCTTCGTCCAGTACTTCACAAACCGCT TCCCACGGCTGCTCCTCCACACGCACCGAGCCATGAGGAGCTGCGCCTCTGAGAGCCTCTTCCTGCCCTA CTACCCGCCAGACTCAGAGGCCAGGAGGCCATGCCCTGGGGCCACAGGGAGGTGA >OriGene 5' read for mutant NM_033266 unedited
ACGCCCTTCCCGCAACGGGCGGAGGCGTAACGGAGGGAGGACACATAAGCAGAGCACGAACAGCGAACCG TCAGAACAAAGTAATACGACACACAATAGGGCGGCCGCGAAAACGGCACCAGCATCGAAGGACCAATGTA CGACACAGAAACGGCCTTTCTCTCTGACCCAGCAGATGGCAGCCTGTACATCTTGGGGACCCAAAAACAA CAGGGATTAATGAAACTGCCATTTCACCATCCCTGAGCTGGTTTCATGCCTCTCCCTGCCGCAGCTCTGA TGGGGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTTGTGGTGGACCCTGAGTCAGGGGAGACCCA GATGACACTGACCACAGAAGGGTCCCCTCCAACCCCCCCGCCTCTACATTGCCCGAACCACAGTATACGG TCACCATGGCATGAACCCAAGAGCCCCCAGCCCTGCCGCTGGAACCACCACCTACCCGCGCTACTCAGCC GCCCCCCCATGGGATGCCTAACCTGGGGAAAATCCATGGACCCACCTGGCCGTCCCTGCGGGATGGGGCC TGGCTGCTTAACGTTGGACCCCAGAAGCGGGACGTTCCTTTGAACAAGGACCCTGGCGTTGCCTGTGAAT GGCCTTTCACCCTGGCACCCAGACGGGCTGGCCAGCTTCGCGATTTTAAGCTGGGTCGGACACTCTGCAA TTCCGCCTCTGCTGTGGGCCATTCGAATGCCTGCGCTAGGCCCCGGAACAACACCCTTTCTACTTGGACA CCACTGCTATAGACCTTAGTGGAGGATAACCGGTCTATGTTTAAGACTGGTCACGATGCCTGTCCTTGAT GACTGCCCGATGCCACAGATGTACTCAGTCAGACCAAGTCAACGACTGGTATCTACGTGCTCACAT >SC323535 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TCATGAGTGCTGGGCCGCGGGGCAGGCGGGACTTTCGTTTTCCGGGGACAGTTTGAGGGACGGGCAGTGG CTGTCATGCGGCTCCTCCGCGAGTGCTTTGGCCTGGTTCGGCGGGAAGTTCAACTGCTGCAGGAGTCTGA CAGGCACCCCAACGTGCTCCGCTACTTCTGCACCGAGCGGGGACCCCAGTTCCACTACATTGCCCTGGAG CTCTGCCGGGCCTCCTTGCAGGAGTACGTAGAAAACCCGGACCTG |
| Restriction Sites | Please inquire |
| ACCN | NM_033266 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033266.2, NP_150296.2 |
| RefSeq Size | 3549 bp |
| RefSeq ORF | 2925 bp |
| Locus ID | 10595 |
| UniProt ID | Q76MJ5 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224780 | ERN2 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 2 (ERN2) |
CNY 7,136.00 |
|
| RC224780L1 | Lenti ORF clone of Human endoplasmic reticulum to nucleus signaling 2 (ERN2), Myc-DDK-tagged |
CNY 9,536.00 |
|
| RC224780L2 | Lenti ORF clone of Human endoplasmic reticulum to nucleus signaling 2 (ERN2), mGFP tagged |
CNY 9,030.00 |
|
| RC224780L3 | Lenti ORF clone of Human endoplasmic reticulum to nucleus signaling 2 (ERN2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC224780L4 | Lenti ORF clone of Human endoplasmic reticulum to nucleus signaling 2 (ERN2), mGFP tagged |
CNY 9,030.00 |
|
| RG224780 | ERN2 (tGFP-tagged) - Human endoplasmic reticulum to nucleus signaling 2 (ERN2) |
CNY 8,736.00 |
|
| SC308731 | ERN2 (untagged)-Human endoplasmic reticulum to nucleus signaling 2 (ERN2) |
CNY 7,144.00 |
