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BUB1 (NM_004336) Human Untagged Clone
CAT＃: SC323526
BUB1 (untagged)-Kinase deficient mutant (K821M) of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)
CNY 7,560.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BUB1A; BUB1L; hBUB1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323526 sequence for NM_004336 edited (data generated by NextGen Sequencing)
ATGGACACCCCGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGC AATGACCCTCTTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAG AATAAAGAATACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAG AAATACCACAATGACCCAAGATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGT GACCTCCATCAATTTTTTGAGTTTCTGTACAACCATGGGATTGGAACCCTGTCATCCCCT CTGTACATTGCCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCATGCCAGTGCT GTCCTTCAGAGAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATAC AGGTTATTTCAGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAA CCTCTGCATAATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAAT AACATGGCCTGCATTTCTAAGAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCT TGTGATAAAGAGTCAAATATGGAACGAAGAGTGATCACGATTTCTAAATCAGAATATTCT GTGCACTCATCTTTGGCATCCAAAGTTGATGTTGAGCAGGTTGTTATGTATTGCAAGGAG AAGCTTATTCGTGGGGAATCAGAATTTTCCTTTGAAGAATTGAGAGCCCAGAAATACAAT CAACGGAGAAAGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAAAAGGAAAGAA GCAAATGCTTTTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTG CATCAGGTGGTGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTT AATCCAGCACGTATGGGGCCAAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTT CCAGTAACCTATCAGCAGACACCAGTGAACATGGAAAAGAACCCAAGAGAGGCACCTCCT GTTGTTCCTCCTTTGGCAAATGCTATTTCTGCAGCTTTGGTGTCCCCAGCCACCAGCCAG AGCATTGCTCCTCCTGTTCCTTTGAAAGCCCAGACAGTAACAGACTCCATGTTTGCAGTG GCCAGCAAAGATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCCACAGAGTGGA GCAGAGATCAAAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACA ACTCCAAACACATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCC ACCGTGCACACAAAAGAAGCATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTT CCTGATATTTCTGATGACAAAGATGAATGGCAATCTCTAGATCAAAATGAAGATGCATTT GAAGCCCAGTTTCAAAAAAATGTAAGGTCATCTGGGGCTTGGGGAGTCAATAAGATCATC TCTTCTTTGTCATCTGCTTTTCATGTGTTTGAAGATGGAAACAAAGAAAATTATGGATTA CCACAGCCTAAAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTCTGTCAGCAGA CTTCCTTCAAAACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACT GTATGGGGTATTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTC ACATCTGCTGCACAACTTGCGTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCAC ATTTTAGAAGATAAAGAAAATGTGGTAGCAAAACAGTGTACCCAGGCGACTTTGGATTCT TGTGAGGAAAACATGGTGGTGCCTTCAAGGGATGGAAAATTCAGTCCAATTCAAGAGAAA AGCCCAAAACAGGCCTTGTCGTCTCACATGTATTCAGCATCCTTACTTCGTCTGAGCCAG CCTGCTGCAGGTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGCTTGCAGACTC ACAGACACTGACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCA GAATGGATGCAGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAAC CCATGGGATGATAAGCTGATTTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCC TATCCAAATACTTTTGAATGGCAATGTAAACTTCCAGCCATCAAGCCCAAGACTGAATTT CAATTGGGTTCTAAGCTGGTCTATGTCCATCACCTTCTTGGAGAAGGAGCCTTTGCCCAG GTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAAATTTGTTTTA ATGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGA CTAAAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAAT GGCAGTGTATTAGTAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACCTC TATAAAAATACCCCTGAAAAAGTGATGCCTCAAGGTCTTGTCATCTCTTTTGCTATGAGA ATGCTTTACATGATTGAGCAAGTGCATGACTGTGAAATCATTCATGGAGACATTAAACCA GACAATTTCATACTTGGAAACGGATTTTTGGAACAGGATGATGAAGATGATTTATCTGCT GGCTTGGCACTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTTCCAAAAGGAACT ATATTCACAGCAAAGTGTGAAACATCTGGTTTTCAGTGTGTTGAGATGCTCAGCAACAAA CCATGGAACTACCAGATCGATTACTTTGGGGTTGCTGCAACAGTATATTGCATGCTCTTT GGCACTTACATGAAAGTGAAAAATGAAGGAGGAGAGTGTAAGCCTGAAGGTCTTTTTAGA AGGCTTCCTCATTTGGATATGTGGAATGAATTTTTTCATGTTATGTTGAATATTCCAGAT TGTCATCATCTTCCATCTTTGGATTTGTTAAGGCAAAAGCTGAAGAAAGTATTTCAACAA CACTATACTAACAAGATTAGGGCCCTACGTAATAGGCTAATTGTACTGCTCTTAGAATGT AAGCGTTCACGAAAATAA Clone variation with respect to NM_004336.3 2462 a=>t >OriGene 5' read for mutant NM_004336 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTTCTAGTTTGC GGTTCAGGTTTGGCCGCTGCCGGCCAGCGTCCTCTGGCCATGGACACCCCGGAAAATGTCCTTCAGATGC TTGAAGCCCACATGCAGAGCTACAAGGGCAATGACCCTCTTGGTGAATGGGAAAGATACATACAGTGGGT AGAAGAGAATTTTCCTGAGAATAAAGAATACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTA GATAAGAAAGAAAATACCACAATGACCCAAGATTCATCAAGTTAATTGTTTAAAATTTGCTGAGTACAAC AGTTGACCCTCCATCATTTTTTTGAAGTTTTCTGTACCAACCCATGGGGATGGGACCCCTGGTCATCCCC CTTCTGTACATTGGCTGGGCCGGGGCATTCTGGAAGCCCAAGGAAGACCTGCGCATGGCAGGGCTGTCCT TCGAGAGGGAATTCAAAACCAGGCTGGACCCAGAGAGATTCCTCGACACACATACAGGTTATTTACGACC GGCTTACTGAAACCACATTTGGCAGCTAAGCTAACCTCAAACCTCGCATAGTCAAGTTATCAGTAACCTC ATCATTCGGAAAACTGCGCGATTCTAGAACAGGTCAGCCTCGAGATCTCACCTGTGCAGTCATGGACAAG TCAATTACGAGCTGGCCACTGACCAATGTGACCCGTAGATCCAGAAACTTCGATATA >SC323526 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TTGCTAGRCTTTGCCAGGTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAAATT TGTTTTAATGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGACTA AAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAATGGCAGTGTATTAG TAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACC |
| Restriction Sites | Please inquire |
| ACCN | NM_004336 |
| Insert Size | 3900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004336.2, NP_004327.1 |
| RefSeq Size | 3486 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 699 |
| UniProt ID | O43683 |
| Domains | pkinase, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207013 | BUB1 (Myc-DDK-tagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 7,552.00 |
|
| RC207013L1 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), Myc-DDK-tagged |
CNY 9,952.00 |
|
| RC207013L2 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), mGFP tagged |
CNY 9,952.00 |
|
| RC207013L3 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC207013L4 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), mGFP tagged |
CNY 10,260.00 |
|
| RG207013 | BUB1 (tGFP-tagged) - Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 9,152.00 |
|
| SC110841 | BUB1 (untagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 7,560.00 |
