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MEK3 (MAP2K3) (NM_145109) Human Untagged Clone
CAT＃: SC323493
MAP2K3 (untagged)-Kinase deficient mutant (K93M) of Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323493 sequence for NM_145109 edited (data generated by NextGen Sequencing)
ATGGAGTCGCCCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCAAAGGAAAATCCAAG AGGAAGAAGGATCTACGGATATCCTGCATGTCCAAGCCACCCGCACCCAACCCCACACCC CCCCGGAACCTGGACTCCCGGACCTTCATCACCATTGGAGACAGAAACTTTGAGGTGGAG GCTGATGACTTGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATGGGGTGGTAGAGAAG GTGCGGCACGCCCAGAGCGGCACCATCATGGCCGTGATGCGGATCCGGGCCACCGTGAAC TCACAGGAGCAGAAGCGGCTGCTCATGGACCTGGACATCAACATGCGCACGGTCGACTGT TTCTACACTGTCACCTTCTACGGGGCACTATTCAGAGAGGGAGACGTGTGGATCTGCATG GAGCTCATGGACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGGATAAAAACATGACA ATTCCAGAGGACATCCTTGGGGAGATTGCTGTGTCTATCGTGCGGGCCCTGGAGCATCTG CACAGCAAGCTGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATGTCCTTATCAACAAG GAGGGCCATGTGAAGATGTGTGACTTTGGCATCAGTGGCTACTTGGTGGACTCTGTGGCC AAGACGATGGATGCCGGCTGCAAGCCCTACATGGCCCCTGAGAGGATCAACCCAGAGCTG AACCAGAAGGGCTACAATGTCAAGTCCGACGTCTGGAGCCTGGGCATCACCATGATTGAG ATGGCCATCCTGCGGTTCCCTTACGAGTCCTGGGGGACCCCGTTCCAGCAGCTGAAGCAG GTGGTGGAGGAGCCGTCCCCCCAGCTCCCAGCCGACCGTTTCTCCCCCGAGTTTGTGGAC TTCACTGCTCAGTGCCTGAGGAAGAACCCCGCAGAGCGTATGAGCTACCTGGAGCTGATG GAGCACCCCTTCTTCACCTTGCACAAAACCAAGAAGACGGACATTGCTGCCTTCGTGAAG GAGATCCTGGGAGAAGACTCATAG Clone variation with respect to NM_145109.2 278 a=>t >OriGene 5' read for mutant NM_145109 unedited
ACCGCCCGTTCTCAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTA GTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGTC GCGGACTCGTCCTTGCTGCAGTCGCCGCCGCAGTCCTCGCCGCAGTCGCCGCCGCCGCCGCCGCCGCCGC CGCTGCTCCTCCGCCTGGCCTGGGCCGTCTGCCCGCAGCCATGAGCGTGCTCGGCCCCGGTGGAGCCCGC AGTCCTCTAGATTAGTCTCCCCCCGCCGTCCAGGACCCACTTGCAGCATGGAGTCGCCCGCCTCGGGCCC GGCGCCGCCAGCATGCCCCCATTCCAAAGGAAAATCCAAGAGAAAAAAGGATCTCCGGAAAGTCCTGGGT GTTCCAACCACCCGCCCCTGACCCCAAACCCCCGGAAACCTGGAACCCCGAGCCCTCATTCACATTTAAA AACAAAGCCTATGAAGGGAGGGCAGGA >SC323493 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TKTCWTCTMSACTGGGCGTGGAGCCTATGGGGTGGTAGAGAAGGTGCGGCACGCCCAGAGCGGCACCWTC ATGGCCGTGATGCGGATCCGGGCCACCGTGAACTCACAGGAGCAGAAGCGGCTGCTCATGGACCTGGACA TCAACATGCGCACGGTCGACTGTTTCTACACTGTCACCTTCTACGGGGCACTATTCARAGAGGGAGACGT GTGGATCTGCATGGAGCTCATGGACACATCCTTGGACAAGTTCTA |
| Restriction Sites | Please inquire |
| ACCN | NM_145109 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145109.1, NP_659731.1 |
| RefSeq Size | 2024 bp |
| RefSeq ORF | 1044 bp |
| Locus ID | 5606 |
| UniProt ID | P46734 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (B) encodes the longer isoform (B). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207115 | MAP2K3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B |
CNY 3,656.00 |
|
| RC207115L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207115L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B, mGFP tagged |
CNY 5,890.00 |
|
| RC207115L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207115L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B, mGFP tagged |
CNY 5,890.00 |
|
| RG207115 | MAP2K3 (tGFP-tagged) - Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B |
CNY 5,256.00 |
|
| SC109628 | MAP2K3 (untagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B |
CNY 3,656.00 |
