Protein Kinase D2 (PRKD2) (NM_001079882) Human Untagged Clone

CAT#: SC323487

PRKD2 (untagged)-Kinase deficient mutant (K580M) of Human protein kinase D2 (PRKD2), transcript variant 4



  "NM_001079882" in other vectors (5)

CNY 5,288.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-PRKD2 (Protein Kinase D2) mouse monoclonal antibody, clone OTI5G1 (formerly 5G1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HSPC187; nPKC-D2; PKD2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323487 sequence for NM_001079882 edited (data generated by NextGen Sequencing)
ATGCTCTTCGGCCTAGTGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAACTACCAC
AAGCGCTGTGCCTTCAGCATCCCCAACAACTGTAGTGGGGCCCGCAAACGGCGCCTGTCA
TCCACGTCTCTGGCCAGTGGCCACTCGGTGCGCCTCGGCACCTCCGAGTCCCTGCCCTGC
ACGGCTGAAGAGCTGAGCCGTAGCACCACCGAACTCCTGCCTCGCCGTCCCCCGTCATCC
TCTTCCTCCTCTTCTGCCTCATCGTATACGGGCCGCCCCATTGAGCTGGACAAGATGCTG
CTCTCCAAGGTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACGGCCCACCGTT
TGCCAGGCTTGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGAC
TGCAAGTTTAACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAG
GCCCTTATCAATGGAGATGTGCCGATGGAGGAGGCCACCGATTTCAGCGAGGCTGACAAG
AGCGCCCTCATGGATGAGTCAGAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAAT
GCGCTCCACGCCAGTGAGGAGGAGGAAGGCGAGGGAGGCAAGGCCCAGAGCTCCCTGGGG
TACATCCCCCTAATGAGGGTGGTGCAATCGGTGCGACACACGACGCGGAAATCCAGCACC
ACGCTGCGGGAGGGTTGGGTGGTTCATTACAGCAACAAGGACACGCTGAGAAAGCGGCAC
TATTGGCGCCTGGACTGCAAGTGTATCACGCTCTTCCAGAACAACACGACCAACAGATAC
TATAAGGAAATTCCGCTGTCAGAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTT
GTGCCGCCGGGCACCAACCCACACTGCTTTGAGATCGTCACTGCCAATGCCACCTACTTC
GTGGGCGAGATGCCTGGCGGGACTCCGGGTGGGCCAAGTGGGCAGGGGGCTGAGGCCGCC
CGGGGCTGGGAGACAGCCATCCGCCAGGCCCTGATGCCCGTCATCCTTCAGGACGCACCC
AGCGCCCCAGGCCACGCGCCCCACAGACAAGCTTCTCTGAGCATCTCTGTGTCCAACAGT
CAGATCCAAGAGAATGTGGACATTGCCACTGTCTACCAGATCTTCCCTGACGAAGTGCTG
GGCTCAGGGCAGTTTGGAGTGGTCTATGGAGGAAAACACCGGAAGACAGGCCGGGACGTG
GCAGTTAAGGTCATTGACAAACTGCGCTTCCCTACCAAGCAGGAGAGCCAGCTCCGGAAT
GAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTGGAGTGCATGTTC
GAGACGCCTGAGAAAGTGTTTGTGGTGATGGAGAAGCTGCATGGGGACATGTTGGAGATG
ATCCTGTCCAGTGAGAAGGGCCGGCTGCCTGAGCGCCTCACCAAGTTCCTCATCACCCAG
ATCCTGGTGGCTTTGAGACACCTTCACTTCAAGAACATTGTCCACTGTGACTTGAAACCA
GAAAACGTGTTGCTGGCATCAGCAGACCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGC
TTTGCTCGCATCATCGGCGAGAAGTCGTTCCGCCGCTCAGTGGTGGGCACGCCGGCCTAC
CTGGCACCCGAGGTGCTGCTCAACCAGGGCTACAACCGCTCGCTGGACATGTGGTCAGTG
GGCGTGATCATGTACGTCAGCCTCAGCGGCACCTTCCCTTTCAACGAGGATGAGGACATC
AATGACCAGATCCAGAACGCCGCCTTCATGTACCCGGCCAGCCCCTGGAGCCACATCTCA
GCTGGAGCCATTGACCTCATCAACAACCTGCTGCAGGTGAAGATGCGCAAACGCTACAGC
GTGGACAAATCTCTCAGCCACCCCTGGTTACAGGAGTACCAGACGTGGCTGGACCTCCGA
GAGCTGGAGGGGAAGATGGGAGAGCGATACATCACGCATGAGAGTGACGACGCGCGCTGG
GAGCAGTTTGCAGCAGAGCATCCGCTGCCTGGGTCTGGGCTGCCCACGGACAGGGATCTC
GGTGGGGCCTGTCCACCACAGGACCACGACATGCAGGGGCTGGCGGAGCGCATCAGTGTT
CTCTGA

Clone variation with respect to NM_001079882.1
>OriGene 5' read for mutant NM_001079882 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG
ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCCACCCCAGCT
CCCCCTTCCAGCCACCAAACCCACGCCGGCGCCCCCTCCCCGTACAATTGGGGCGCTGGCATCCTGCCCG
GCTCGCGCTGGGGTTGGGAGGGGGCAGGCAGGAAGCGAGGGCCTGCGGGGTCTCTGCGTTTCCGGGGGAA
ACAGCCGGCCCTGCCCTGGGAGGGTCACAGTCCGCCCGCTGCTGAAGGCGGGCTCTGGAGGCTTTTCCGT
CGCCACATCCCCTCTCCCCGCCCCCTTCAGTTTCCCTGGAGTTGTGGGCTTTCTTACGGGCCTTAACAAC
AAGAATCCTGCCTTTTTCAAAACATGGACCCCACCTTCGGCAAACCTCTGCAACCTTGTGTGCCCCTCGT
CCGGAGCATTCCAGAAGGGCACCCTGTTGAAGTTGTTGCTGTCGGCCTGGCCCACTTTCAGGAACTTCCA
AACCCCCCCCCCCCCCCAGGGGCACTCATTCGGGGCGCGTGCTTTGTGGATCTGGGGGGAAAACCCTTTT
GGCCCTAGGGCCCGGGCCCAAGGGCAGGGGCGGGCCTGAAACACAAACGCTGCCTCTTCACACCAAACTG
TGTGGGGCCCCCACACGGCCCGGTCACATTCTGGAGCGGGGCAATTGTCCCTGCGACTCAGTACTCCCGC
GCGGAGTGTAATAGAGATATAACAGCGCTTCCGCGGCGCGC
>SC323487 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGCMTTKACACTGTCTACCAGATCTTCCCTGACGAAGTGCTGGGCTCAGGGCAGTTTGGAGTGGTCTATG
GAGGAAAACACCGGAAGACAGGCCGGGACGTGGCAGTTATGGTCATTGACAAACTGCGCTTCCCTACCAA
GCAGGAGAGCCAGCTCCGGAATGAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTG
GAGTGCATGTTCGAGACGCCTGAGAAAGTGTTTGTGGTGATGGAG
Restriction Sites Please inquire     
ACCN NM_001079882
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001079882.1, NP_001073351.1
RefSeq Size 2939 bp
RefSeq ORF 2166 bp
Locus ID 25865
UniProt ID Q9BZL6
Protein Families Druggable Genome, Protein Kinase
Gene Summary The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (4) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (B) has a shorter N-terminus when compared to isoform A.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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