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Protein Kinase D2 (PRKD2) (NM_001079882) Human Untagged Clone
CAT＃: SC323487
PRKD2 (untagged)-Kinase deficient mutant (K580M) of Human protein kinase D2 (PRKD2), transcript variant 4
CNY 5,288.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPC187; nPKC-D2; PKD2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323487 sequence for NM_001079882 edited (data generated by NextGen Sequencing)
ATGCTCTTCGGCCTAGTGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAACTACCAC AAGCGCTGTGCCTTCAGCATCCCCAACAACTGTAGTGGGGCCCGCAAACGGCGCCTGTCA TCCACGTCTCTGGCCAGTGGCCACTCGGTGCGCCTCGGCACCTCCGAGTCCCTGCCCTGC ACGGCTGAAGAGCTGAGCCGTAGCACCACCGAACTCCTGCCTCGCCGTCCCCCGTCATCC TCTTCCTCCTCTTCTGCCTCATCGTATACGGGCCGCCCCATTGAGCTGGACAAGATGCTG CTCTCCAAGGTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACGGCCCACCGTT TGCCAGGCTTGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGAC TGCAAGTTTAACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAG GCCCTTATCAATGGAGATGTGCCGATGGAGGAGGCCACCGATTTCAGCGAGGCTGACAAG AGCGCCCTCATGGATGAGTCAGAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAAT GCGCTCCACGCCAGTGAGGAGGAGGAAGGCGAGGGAGGCAAGGCCCAGAGCTCCCTGGGG TACATCCCCCTAATGAGGGTGGTGCAATCGGTGCGACACACGACGCGGAAATCCAGCACC ACGCTGCGGGAGGGTTGGGTGGTTCATTACAGCAACAAGGACACGCTGAGAAAGCGGCAC TATTGGCGCCTGGACTGCAAGTGTATCACGCTCTTCCAGAACAACACGACCAACAGATAC TATAAGGAAATTCCGCTGTCAGAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTT GTGCCGCCGGGCACCAACCCACACTGCTTTGAGATCGTCACTGCCAATGCCACCTACTTC GTGGGCGAGATGCCTGGCGGGACTCCGGGTGGGCCAAGTGGGCAGGGGGCTGAGGCCGCC CGGGGCTGGGAGACAGCCATCCGCCAGGCCCTGATGCCCGTCATCCTTCAGGACGCACCC AGCGCCCCAGGCCACGCGCCCCACAGACAAGCTTCTCTGAGCATCTCTGTGTCCAACAGT CAGATCCAAGAGAATGTGGACATTGCCACTGTCTACCAGATCTTCCCTGACGAAGTGCTG GGCTCAGGGCAGTTTGGAGTGGTCTATGGAGGAAAACACCGGAAGACAGGCCGGGACGTG GCAGTTAAGGTCATTGACAAACTGCGCTTCCCTACCAAGCAGGAGAGCCAGCTCCGGAAT GAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTGGAGTGCATGTTC GAGACGCCTGAGAAAGTGTTTGTGGTGATGGAGAAGCTGCATGGGGACATGTTGGAGATG ATCCTGTCCAGTGAGAAGGGCCGGCTGCCTGAGCGCCTCACCAAGTTCCTCATCACCCAG ATCCTGGTGGCTTTGAGACACCTTCACTTCAAGAACATTGTCCACTGTGACTTGAAACCA GAAAACGTGTTGCTGGCATCAGCAGACCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGC TTTGCTCGCATCATCGGCGAGAAGTCGTTCCGCCGCTCAGTGGTGGGCACGCCGGCCTAC CTGGCACCCGAGGTGCTGCTCAACCAGGGCTACAACCGCTCGCTGGACATGTGGTCAGTG GGCGTGATCATGTACGTCAGCCTCAGCGGCACCTTCCCTTTCAACGAGGATGAGGACATC AATGACCAGATCCAGAACGCCGCCTTCATGTACCCGGCCAGCCCCTGGAGCCACATCTCA GCTGGAGCCATTGACCTCATCAACAACCTGCTGCAGGTGAAGATGCGCAAACGCTACAGC GTGGACAAATCTCTCAGCCACCCCTGGTTACAGGAGTACCAGACGTGGCTGGACCTCCGA GAGCTGGAGGGGAAGATGGGAGAGCGATACATCACGCATGAGAGTGACGACGCGCGCTGG GAGCAGTTTGCAGCAGAGCATCCGCTGCCTGGGTCTGGGCTGCCCACGGACAGGGATCTC GGTGGGGCCTGTCCACCACAGGACCACGACATGCAGGGGCTGGCGGAGCGCATCAGTGTT CTCTGA Clone variation with respect to NM_001079882.1 >OriGene 5' read for mutant NM_001079882 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCCACCCCAGCT CCCCCTTCCAGCCACCAAACCCACGCCGGCGCCCCCTCCCCGTACAATTGGGGCGCTGGCATCCTGCCCG GCTCGCGCTGGGGTTGGGAGGGGGCAGGCAGGAAGCGAGGGCCTGCGGGGTCTCTGCGTTTCCGGGGGAA ACAGCCGGCCCTGCCCTGGGAGGGTCACAGTCCGCCCGCTGCTGAAGGCGGGCTCTGGAGGCTTTTCCGT CGCCACATCCCCTCTCCCCGCCCCCTTCAGTTTCCCTGGAGTTGTGGGCTTTCTTACGGGCCTTAACAAC AAGAATCCTGCCTTTTTCAAAACATGGACCCCACCTTCGGCAAACCTCTGCAACCTTGTGTGCCCCTCGT CCGGAGCATTCCAGAAGGGCACCCTGTTGAAGTTGTTGCTGTCGGCCTGGCCCACTTTCAGGAACTTCCA AACCCCCCCCCCCCCCCAGGGGCACTCATTCGGGGCGCGTGCTTTGTGGATCTGGGGGGAAAACCCTTTT GGCCCTAGGGCCCGGGCCCAAGGGCAGGGGCGGGCCTGAAACACAAACGCTGCCTCTTCACACCAAACTG TGTGGGGCCCCCACACGGCCCGGTCACATTCTGGAGCGGGGCAATTGTCCCTGCGACTCAGTACTCCCGC GCGGAGTGTAATAGAGATATAACAGCGCTTCCGCGGCGCGC >SC323487 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGCMTTKACACTGTCTACCAGATCTTCCCTGACGAAGTGCTGGGCTCAGGGCAGTTTGGAGTGGTCTATG GAGGAAAACACCGGAAGACAGGCCGGGACGTGGCAGTTATGGTCATTGACAAACTGCGCTTCCCTACCAA GCAGGAGAGCCAGCTCCGGAATGAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTG GAGTGCATGTTCGAGACGCCTGAGAAAGTGTTTGTGGTGATGGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001079882 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001079882.1, NP_001073351.1 |
| RefSeq Size | 2939 bp |
| RefSeq ORF | 2166 bp |
| Locus ID | 25865 |
| UniProt ID | Q9BZL6 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (B) has a shorter N-terminus when compared to isoform A. |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214956 | PRKD2 (Myc-DDK-tagged)-Human protein kinase D2 (PRKD2), transcript variant 4 |
CNY 5,800.00 |
|
| RC214956L3 | Lenti-ORF clone of PRKD2 (Myc-DDK-tagged)-Human protein kinase D2 (PRKD2), transcript variant 4 |
CNY 7,700.00 |
|
| RC214956L4 | Lenti-ORF clone of PRKD2 (mGFP-tagged)-Human protein kinase D2 (PRKD2), transcript variant 4 |
CNY 7,700.00 |
|
| RG214956 | PRKD2 (tGFP-tagged) - Human protein kinase D2 (PRKD2), transcript variant 4 |
CNY 6,370.00 |
|
| SC315827 | PRKD2 (untagged)-Human protein kinase D2 (PRKD2), transcript variant 4 |
CNY 13,870.00 |
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