United States
Germany
Japan
United Kingdom
China
MAPK15 (NM_139021) Human Untagged Clone
CAT＃: SC323484
MAPK15 (untagged)-Kinase deficient mutant (K42M) of Human mitogen-activated protein kinase 15 (MAPK15)
CNY 2,400.00
CNY 8,740.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ERK7; ERK8 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_139021, the custom clone sequence may differ by one or more nucleotides
ATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGGCAGGGGGCCT ATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGC TTTTAGGGATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGAGTTTGGGGACCAT CCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAGAGAACGACAGGGACATTTACCTGGTGTTTGAGT TTATGGACACTGACCTGAACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCAT CTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTGTGCACCGGGACCAGAAGCCG TCCAATGTGCTCCTGGATGCCAACTGCACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCG ACCTCCCCGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGTACCGAGCACCGGA GGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGACATGTGGAGTCTGGGCTGTATCCTGGGGGAG ATGCTGCGGGGGAGACCCCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCA TCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGCCGTGCCTCTGTGCTGCACCAGCT GGGGTCCCGGCCACGACAGACGCTGGATGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTC CTTAGGCGACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAGCACCCCTACG TGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGAGGCAGATGTGCGGCCCCGGGCACACGAAGG GGTCCAGCTCTCTGTGCCTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGCAGC GGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGCACACCTGCACAAACCCAGAGCCG ACCCTCAGCTGCCTTCTAGGACACCTGTGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGA CCCTGCCGAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCCCCTGCTCCAA ACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGGCGAAGGAAGCGCCCCCCTTGACACTCTCGCTGG TGAAGCCAAGCGGGAGGGGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCAGGC CCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCGTACAACAGGTCCCTCCCCGG CTTCCTCCGGAGGCCCGGCCCGGCCGGAGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTG CCAGGGCTTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGGGCCACCTGCC CCTGCTGGAGGGGCACCATGTGTGA >OriGene 5' read for mutant NM_139021 unedited
CCCGCCCGTCTCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATCGACTCAACAGT AAGGCCCCGCGGGCGTCCTGGCCGCCATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACT CAGGCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTC GTGGCCATCATGAAAATCTTTGATGCTTTTTAGGGATAAGACAGATGCCCCAGAGAACATTTCCGGGAAA TCACGCTCCCTCCCAGGAGTTTTGGGGGACCCATCCCCAACATCATCAGCCCTCCTTGACGTTGATCCCG GGCAGAAGAACGAACAGGGACATTTTACCCTGGTTGTTTGAAGTTTTATGGTTTGCCCCCCCCAGCCCCC CCACCCCCGGACTGGAGTTGGCGCACCCTTCTGCAAACCTGACCTGAACGCAGTATTCCGGAAGGGCCGG CCGGGTGAGGAACTTCCCGTGCGTCACTTTTCACAAGTTCGGGGGCCACCGGTTCCTCACTCGGGAATGT GTGCCCGGACCAACCTCCAATGGTCTCGGATGCATTGCATGAACCTTGAATTGCCGGCGCGCCGGGAACT CCTGAGGCCTGGCCGCCGTGAAATCTTGCCACCGATCACACGGGGGGGCT >SC323484 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSATKMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATCGACTCAACAGTAAGGCCCCGC GGGCGTCCTGGCCGCCATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAG CTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGG |
| Restriction Sites | Please inquire |
| ACCN | NM_139021 |
| Insert Size | 2328 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139021.1, NP_620590.1 |
| RefSeq Size | 1904 bp |
| RefSeq ORF | 834 bp |
| Locus ID | 225689 |
| UniProt ID | Q8TD08 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:22948227, PubMed:24618899, PubMed:29021280, PubMed:21847093, PubMed:20733054). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3' UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:20638370, PubMed:19166846). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216589 | MAPK15 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 15 (MAPK15) |
CNY 4,048.00 |
|
| RC216589L1 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), Myc-DDK-tagged |
CNY 6,448.00 |
|
| RC216589L2 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), mGFP tagged |
CNY 6,650.00 |
|
| RC216589L3 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), Myc-DDK-tagged |
CNY 6,650.00 |
|
| RC216589L4 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), mGFP tagged |
CNY 6,448.00 |
|
| RG216589 | MAPK15 (tGFP-tagged) - Human mitogen-activated protein kinase 15 (MAPK15) |
CNY 5,648.00 |
|
| SC120623 | MAPK15 (untagged)-Human mitogen-activated protein kinase 15 (MAPK15) |
CNY 2,400.00 |
|
| SC317926 | MAPK15 (untagged)-Human mitogen-activated protein kinase 15 (MAPK15) |
CNY 8,740.00 |
