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DRAK2 (STK17B) (NM_004226) Human Untagged Clone
CAT＃: SC323478
STK17B (untagged)-Kinase deficient mutant (K62M) of Human serine/threonine kinase 17b (STK17B)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DRAK2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323478 sequence for NM_004226 edited (data generated by NextGen Sequencing)
ATGTCGAGGAGGAGATTTGATTGCCGAAGTATTTCAGGCCTACTAACTACAACTCCTCAA ATTCCAATAAAAATGGAAAACTTTAATAATTTCTATATACTTACATCTAAAGAGCTAGGG AGAGGTAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGCCAAGAATATGCT GCAATGTTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGGGCAGAAATTTTACACGAG ATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTAT GAAAATACAAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAATTTTCAGC CTGTGTTTACCTGAGTTGGCTGAAATGGTTTCTGAAAATGATGTTATCAGACTCATTAAA CAAATACTTGAAGGAGTTTATTATCTACATCAGAATAACATTGTACACCTTGATTTAAAG CCACAGAATATATTACTGAGCAGCATATACCCTCTCGGGGACATTAAAATAGTAGATTTT GGAATGTCTCGAAAAATAGGGCATGCGTGTGAACTTCGGGAAATCATGGGAACACCAGAA TATTTAGCTCCAGAAATCCTGAACTATGATCCCATTACCACAGCAACAGATATGTGGAAT ATTGGTATAATAGCATATATGTTGTTAACTCACACATCACCATTTGTGGGAGAAGATAAT CAAGAAACATACCTCAATATCTCTCAAGTTAATGTAGATTATTCGGAAGAAACTTTTTCA TCAGTTTCACAGCTGGCCACAGACTTTATTCAGAGCCTTTTAGTAAAAAATCCAGAGAAA AGACCAACAGCAGAGATATGCCTTTCTCATTCTTGGCTACAGCAGTGGGACTTTGAAAAC TTGTTTCACCCTGAAGAAACTTCCAGTTCCTCTCAAACTCAGGATCATTCTGTAAGGTCC TCTGAAGACAAGACTTCTAAATCCTCCTGTAATGGAACCTGTGGTGATAGAGAAGACAAA GAGAATATCCCAGAGGATAGCAGCATGGTTTCCAAAAGATTTCGTTTCGATGACTCATTA CCCAATCCCCATGAACTTGTTTCAGATTTGCTCTGTTAG Clone variation with respect to NM_004226.3 126 a=>t;185 a=>t;186 a=>g;222 a=>g;741 t=>c >OriGene 5' read for mutant NM_004226 unedited
ACGCCCGTCCAGCAACGGGCGGTAGGCGCTGCACGGTGGGAGGTTCTATATAAGCAGAGCCCGTCTAGCG AACCGTCAGAATCCCGTAATACGACCCACTATAGGGCGGCCGCGAATCCGGCACGAGGGCCCTCCCCGCT GCTGTCGCCAGGAGACACTTTCACGAGAAGCCAGGTCACAACCGTCGGCCCTTTGTCTGGAAAAGCAAAA GTGGATCCTGCCACGTTCGGAGCTCCCTGGCGCCTCGCCCGGCTGGAGCTAGAGAACTCGTCCTGTGGCG GCCCCCGGCGTGGGGCGGGACAGCGGCCCCCTGGAGGGGGCAGTCCCGGGAGAACCTGCGGCGGCCGGAG CGGTAAAAATAAGTGACTAAAGAGCAGACCTGGGATCACCTAACATGTCGAGGAAGAGATTTGATTGCCG AGTATTTCAGGCCTACTACCTACCAACTCCTCAAATTCAATAAAAAATGAAAACTTTAATAATTTCTAAT ATACCTACCATCCTAAGGAGCTAGGGAAGAGGTAAATTTGCTTTGGTTAGAACATGGTATTCAAAATCTA ACTGCAAGAATATGCCTGCAATTGTTCTAAAAGAGAGAAAAGGACGGATTGTCCGGCAAAATTTAACCGA ATGCGTGCTGAATTGGCAAGTCTGTCCCGGTATAACTTCTGAGGCTTGAATCATGAAACTTGATGGAATG CCGTGGGAATACCGGTTACGATGCGAAGGTTCGGATAGTTCAACTACACTAGACTAATCCGAACTGACGG GTGAAGCGGATTCAGACAAAATCCGCATAATAG >SC323478 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TCTTACATCTAATGAGCTAGGGAGAGGTAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGC CAAGAATATGCTGCAATGTTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGGGCAGAAATTTTACACG AGATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTATGAAAATAC AAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAAT |
| Restriction Sites | Please inquire |
| ACCN | NM_004226 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004226.2, NP_004217.1 |
| RefSeq Size | 1707 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 9262 |
| UniProt ID | O94768 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204169 | STK17B (Myc-DDK-tagged)-Human serine/threonine kinase 17b (STK17B) |
CNY 3,656.00 |
|
| RC204169L1 | Lenti ORF clone of Human serine/threonine kinase 17b (STK17B), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204169L2 | Lenti ORF clone of Human serine/threonine kinase 17b (STK17B), mGFP tagged |
CNY 5,890.00 |
|
| RC204169L3 | Lenti ORF clone of Human serine/threonine kinase 17b (STK17B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204169L4 | Lenti ORF clone of Human serine/threonine kinase 17b (STK17B), mGFP tagged |
CNY 5,890.00 |
|
| RG204169 | STK17B (tGFP-tagged) - Human serine/threonine kinase 17b (STK17B) |
CNY 4,370.00 |
|
| SC117533 | STK17B (untagged)-Human serine/threonine kinase 17b (STK17B) |
CNY 3,656.00 |
