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Casein Kinase 1 alpha (CSNK1A1) (NM_001892) Human Untagged Clone
CAT＃: SC323470
CSNK1A1 (untagged)-Kinase deficient mutant (K46M) of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CK1; CK1a; CKIa; HEL-S-77p; HLCDGP1; PRO2975 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323470 sequence for NM_001892 edited (data generated by NextGen Sequencing)
ATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGGGAAATATAAACTGGTA CGGAAGATCGGGTCTGGCTCCTTCGGGGACATCTATTTGGCGATCAACATCACCAACGGC GAGGAAGTGGCAGTGAAGCTAGAATCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAG AGCAAGCTCTATAAGATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGT CAGGAAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAAGACCTC TTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTATGTTAGCTGACCAGATG ATCAGTAGAATTGAATATGTGCATACAAAGAATTTTATACACAGAGACATTAAACCAGAT AACTTCCTAATGGGTATTGGGCGTCACTGTAATAAGTTATTCCTTATTGATTTTGGTTTG GCCAAAAAGTACAGAGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAAC CTCACTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAGAGTCGC CGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAATAGAACCAGCCTGCCA TGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAAAATATGAAAAGATTAGTGAAAAGAAG ATGTCCACGCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTA AACTATTGTCGTGGGCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTA TTCCGCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGGACAATG TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA ACCCCCACAGGCAAGCAAACTGACAAAACCAAGAGTAACATGAAAGGTTTCTAA Clone variation with respect to NM_001892.4 >OriGene 5' read for mutant NM_001892 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCT CTGGGCCGGAGCGCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGC CCGCGACGTCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGCCCAGCCTGGTAACCGCC GAGAAGCCCTTCACAAACTGCGGCCTGGCAAAAAGAAACCTGACTGAGCGGCGGTGATCAGGTGTCCCGC TCTGCTGATTCTGGGCCCCGAACCCCCGGTAAAGGCCTCCGTGTTCCGTTTTCCTGCCGCCCCTCCTCCG TAGCCCTTGCCTATGTAGGACCCCCGAGGCCTCCGTCCTCTTCCCAGAGGTTGTCGGGGCTTGCCCCAGC CCTCCATCTTCGTTCTCTAAGGATGGCAAGTAGCAGCGGGTCCAAGGCTGATTTCATTGCCGGGAGGGAA TTTACCTGGTACGGAGATCGGGTCTGGTCCCTCGGGACATATTTTGGCGTTAACTCCCACGGAGGGAATG GGCATGATGCTAATCTAAAGGGCGAGCTCAATGTGTGTCAGAGACAGCTATAGAGATTTCAAGGGGGTGG CCCACCATCGTGTGATGACGGAACACAACAGATATCTCGGCGTA >SC323470 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCTCTGGGCC GGAGCCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGCCCGCGACG TCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGC |
| Restriction Sites | Please inquire |
| ACCN | NM_001892 |
| Insert Size | 2630 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001892.4, NP_001883.4 |
| RefSeq Size | 3065 bp |
| RefSeq ORF | 1014 bp |
| Locus ID | 1452 |
| UniProt ID | P48729 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Protein Pathways | Hedgehog signaling pathway, Wnt signaling pathway |
| Gene Summary | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame internal exon, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201797 | CSNK1A1 (Myc-DDK-tagged)-Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2 |
CNY 3,656.00 |
|
| RC201797L1 | Lenti ORF clone of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201797L2 | Lenti ORF clone of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2, mGFP tagged |
CNY 6,056.00 |
|
| RC201797L3 | Lenti ORF clone of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201797L4 | Lenti ORF clone of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201797 | CSNK1A1 (tGFP-tagged) - Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2 |
CNY 4,370.00 |
|
| SC118967 | CSNK1A1 (untagged)-Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2 |
CNY 3,656.00 |
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