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AMPK alpha 1 (PRKAA1) (NM_006251) Human Untagged Clone
CAT＃: SC323464
PRKAA1 (untagged)-Kinase deficient mutant (K47M) of Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1
CNY 17,088.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMPK; AMPKa1; AMPK alpha 1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006251, the custom clone sequence may differ by one or more nucleotides
ATGCGCAGACTCAGTTCCTGGAGAAAGATGGCGACAGCCGAGAAGCAGAAACACGACGGGCGGGTGAAGA TCGGCCACTACATTCTGGGTGACACGCTGGGGGTCGGCACCTTCGGCAAAGTGAAGGTTGGCAAACATGA ATTGACTGGGCATAAAGTAGCTGTGAAGATACTCAATCGACAGAAGATTCGGAGCCTTGATGTGGTAGGA AAAATCCGCAGAGAAATTCAGAACCTCAAGCTTTTCAGGCATCCTCATATAATTAAACTGTACCAGGTCA TCAGTACACCATCTGATATTTTCATGGTGATGGAATATGTCTCAGGAGGAGAGCTATTTGATTATATCTG TAAGAATGGAAGGCTGGATGAAAAAGAAAGTCGGCGTCTGTTCCAACAGATCCTTTCTGGTGTGGATTAT TGTCACAGGCATATGGTGGTCCATAGAGATTTGAAACCTGAAAATGTCCTGCTTGATGCACACATGAATG CAAAGATAGCTGATTTTGGTCTTTCAAACATGATGTCAGATGGTGAATTTTTAAGAACAAGTTGTGGCTC ACCCAACTATGCTGCACCAGAAGTAATTTCAGGAAGATTGTATGCAGGCCCAGAGGTAGATATATGGAGC AGTGGGGTTATTCTCTATGCTTTATTATGTGGAACCCTTCCATTTGATGATGACCATGTGCCAACTCTTT TTAAGAAGATATGTGATGGGATCTTCTATACCCCTCAATATTTAAATCCTTCTGTGATTAGCCTTTTGAA ACATATGCTGCAGGTGGATCCCATGAAGAGGGCCACAATCAAAGATATCAGGGAACATGAATGGTTTAAA CAGGACCTTCCAAAATATCTCTTTCCTGAGGATCCATCATATAGTTCAACCATGATTGATGATGAAGCCT TAAAAGAAGTATGTGAAAAGTTTGAGTGCTCAGAAGAGGAAGTTCTCAGCTGTCTTTACAACAGAAATCA CCAGGATCCTTTGGCAGTTGCCTACCATCTCATAATAGATAACAGGAGAATAATGAATGAAGCCAAAGAT TTCTATTTGGCGACAAGCCCACCTGATTCTTTTCTTGATGATCATCACCTGACTCGGCCCCATCCTGAAA GAGTACCATTCTTGGTTGCTGAAACACCAAGGGCACGCCATACCCTTGATGAATTAAATCCACAGAAATC CAAACACCAAGGTGTAAGGAAAGCAAAATGGCATTTAGGAATTAGAAGTCAAAGTCGACCAAATGATATT ATGGCAGAAGTATGTAGAGCAATCAAACAATTGGATTATGAATGGAAGGTTGTAAACCCATATTATTTGC GTGTACGAAGGAAGAATCCTGTGACAAGCACTTACTCCAAAATGAGTCTACAGTTATACCAAGTGGATAG TAGAACTTATCTACTGGATTTCCGTAGTATTGATGATGAAATTACAGAAGCCAAATCAGGGACTGCTACT CCACAGAGATCGGGATCAGTTAGCAACTATCGATCTTGCCAAAGGAGTGATTCAGATGCTGAGGCTCAAG GAAAATCCTCAGAAGTTTCTCTTACCTCATCTGTGACCTCACTTGACTCTTCTCCTGTTGACCTAACTCC AAGACCTGGAAGTCACACAATAGAATTTTTTGAGATGTGTGCAAATCTAATTAAAATTCTTGCACAATAA >OriGene 5' read for mutant NM_006251 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCTCGTGCCGAATTC GGCACGAGGCAGCCGAGAAGCAGAAACACGACGGGCGGGTGAAGATCGGCCACTACATTCTGGGTGACAC GCTGGGGGTCGGCACCTTCGGCAAAGTGAAGGTTGGCAAACATGAATTGACTGGGCATAAAGTAGCTGTG ATGATACTCAATCGACAGAAGATTCGGAGCCTTTGATGTGGTAGGGAAAAATCCGCAGAGAAAATTCAGA ACCTCAAGCTTTTTCAGGCATCCCTCAATATAATTAAACTGTACCCAGGTCATCCAGTACACCATTCTGA ATTTTTTCATGGTGATGGGATTATGTTCTAAGGGAGGAAAGCTTTTTTGATATATTCCTTGTAGGAATGA AAGGCTTGGTTGAAAAGGAAGGCCGCCGTCTGTTCCACCCGGATCCTTCCGGTGTGTGATTTATGGCACA CAGCATTAGGGTTGGTCATTGAGAGATTTGAACCCTGAATGTGTCCTGCTGTTATGCAACATGAGAATGC AGATAGCTGAGTTTTGTGTCTTCACATGATATGTCGAAGTGTGATTTTAGAGAACGTGTTGGGGCCACAC CCACTGTGCGCACGAGATATATTTCGAGAGTGTGTGCCAGCCCAAGAGTAATATTGAGACGTGGGTATTC CTAGCGCTATAATGGAACCCTCTATGTGAGAGACACGGGCCACTCTTTTAAGAATTGGTGGGTCTCTACA CCCAGTTATCCTGGTAACCTTGGACATCGCATGGATCGAAGGCATTGAATCGAATGTTAGCTCGATCT >SC323464 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCTCGTGCCGAATTCGGCACG AGGCAGCCGAGAAGCAGAAACACGACGGGCGGGTGAAGATCGGCCACTACATTCTGGGTGACACGCTGGG GGTCGGCACCTTCGGCAAAGTGAAGGTTGGCAAACATGAATTGAC |
| Restriction Sites | Please inquire |
| ACCN | NM_006251 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006251.4, NP_006242.4 |
| RefSeq Size | 5085 bp |
| RefSeq ORF | 5085 bp |
| Locus ID | 5562 |
| UniProt ID | Q13131 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy |
| Gene Summary | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks an in-frame coding exon compared to variant 2. The resulting isoform (1) lacks an internal segment, as compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218572 | PRKAA1 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1 |
CNY 6,240.00 |
|
| RC218572L1 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1, Myc-DDK-tagged |
CNY 8,640.00 |
|
| RC218572L2 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC218572L3 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC218572L4 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1, mGFP tagged |
CNY 8,640.00 |
|
| RG218572 | PRKAA1 (tGFP-tagged) - Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1 |
CNY 7,840.00 |
|
| SC125441 | PRKAA1 (untagged)-Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1 |
CNY 17,088.00 |
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