United States
Germany
Japan
United Kingdom
China
MAPKAP Kinase 3 (MAPKAPK3) (NM_004635) Human Untagged Clone
CAT＃: SC323455
MAPKAPK3 (untagged)-Kinase deficient mutant (K73M) of Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3; MK-3; MK3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323455 sequence for NM_004635 edited (data generated by NextGen Sequencing)
ATGGATGGTGAAACAGCAGAGGAGCAGGGGGGCCCTGTGCCCCCGCCAGTTGCACCCGGC GGACCCGGCTTGGGCGGTGCTCCGGGGGGGCGGCGGGAGCCCAAGAAGTACGCAGTGACC GACGACTACCAGTTGTCCAAGCAGGTGCTGGGCCTGGGTGTGAACGGCAAAGTGCTGGAG TGCTTCCATCGGCGCACTGGACAGAAGTGTGCCCTGATGCTCCTGTATGACAGCCCCAAG GCCCGGCAGGAGGTAGACCATCACTGGCAGGCTTCTGGCGGCCCCCATATTGTCTGCATC CTGGATGTGTATGAGAACATGCACCATGGCAAGCGCTGTCTCCTCATCATCATGGAATGC ATGGAAGGTGGTGAGTTGTTCAGCAGGATTCAGGAGCGTGGCGACCAGGCTTTCACTGAG AGAGAAGCTGCAGAGATAATGCGGGATATTGGCACTGCCATCCAGTTTCTGCACAGCCAT AACATTGCCCACCGAGATGTCAAGCCTGAAAACCTACTCTACACATCTAAGGAGAAAGAC GCAGTGCTTAAGCTCACCGATTTTGGCTTTGCTAAGGAGACCACCCAAAATGCCCTGCAG ACACCCTGCTATACTCCCTATTATGTGGCCCCTGAGGTCCTGGGTCCAGAGAAGTATGAC AAGTCATGTGACATGTGGTCCCTGGGTGTCATCATGTACATCCTCCTTTGTGGCTTCCCA CCCTTCTACTCCAACACGGGCCAGGCCATCTCCCCGGGGATGAAGAGGAGGATTCGCCTG GGCCAGTACGGCTTCCCCAATCCTGAGTGGTCAGAAGTCTCTGAGGATGCCAAGCAGCTG ATCCGCCTCCTGTTGAAGACAGACCCCACAGAGAGGCTGACCATCACTCAGTTCATGAAC CACCCCTGGATCAACCAATCGATGGTAGTGCCACAGACCCCACTCCACACGGCCCGAGTG CTGCAGGAGGACAAAGACCACTGGGACGAAGTCAAGGAGGAGATGACCAGTGCCTTGGCC ACTATGCGGGTAGACTACGACCAGGTGAAGATCAAGGACCTGAAGACCTCTAACAACCGG CTCCTCAACAAGAGGAGAAAAAAGCAGGCAGGCAGCTCCTCTGCCTCACAGGGCTGCAAC AACCAGTAG Clone variation with respect to NM_004635.4 218 a=>t >OriGene 5' read for mutant NM_004635 unedited
ACGCCCGTCCAGCAACGGGCGGAGGCGTCACGGAGGGAGGACACACAAGAGAGCCCGTTAGCGAACCGTC AGAACACCGTAATACGACACACCACAGGGCGGCCGCGAACACGGCACCAGCACCCGCGACCGCCTCTCCC GCCCCTCGCCGGCACCTCAGCAAGGCGCCACTAGAAGCGCCAGGCTGGGGCCGCCTCTGAGCGCCCCGCG GGGGCCATGGATGGTGAAACAGCAGAGGAGCAGGGGGGCCCTGTGCCCCCGCCAGTCGCACCCGGCGGAC CCGGCTTGGGCGGTGCTCCGGGGGGGCGGCGGGGAGCCCAAGAAGTACGCAGTGACCGACGACTACCAGT TGTCCCAAGCAGGTGCTGGGGCCTGGGGTGTGAACGGCAAAGTGCTTGGAGTTGCTTTCCTTCGCCCCAC TGGGACAAAATTGTGCCCTGAATGCTTCTTGTTTAACACCCCCAAGGCCGGGCAGGAGGTAAACAATCAC TGCCGGGCTTTGGGCGGCCCCATTTTTGCTGCCTTCTGGAAGTTTTTGAAAAAACATGCCCATGGGCAGC CCCTGCTTCTTATCCTTATGGGATGGATGGAAAGGGGGGAATTTTGTCAACAAGATTCAAGGGCGTGGCC AACAGGCTTTTACTGAAAAAAAAACTGCAGAGATAATGCGGGAAATTGGCACTCTGCATCCCGTTTCGCA CAGCCATAAAAATGCCCACCCAAATGTGTAAGCCCTGAAAACCTACTCTTCCACATCTTAAGGAGAAAAA AAGCAGTGCTTAAGCCTCACCCGAATTTGGGCTTTTGCTAAGAGACCCACCAAATGCCCTGCAAACCCCC TGCTATACTCCTATATGTGCCCTGAGTTCTGGTCAGAAAGTTGACAGTCATGTGACATGTGTCCTTGTGT CACATGTACTCTCTGTGCTTCCACCTCTACTCACACGCCAGCACTCTCCCAG >SC323455 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TAGCAGGTGCTGGGCCTGGGTKGACGGCAAAGTGCTGGAGTGCTTCCATCGGCGCACTGGACAGAAGTGT GCCCTGATGCTCCTGTATGACAGCCCCAAGGCCCGGCAGGAGGTAGACCATCACTGGCAGGCTTCTGGCG GCCCCCATATTGTCTGCATCCTGGATGTGTATGAGAACATGCACCATGGCAAGCGCTGTCTCCTCATCAT CATGGAATGCATGGAAGGTGGTGAGTTGTTCAGCAGGATTCAGGA |
| Restriction Sites | Please inquire |
| ACCN | NM_004635 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004635.3, NP_004626.1 |
| RefSeq Size | 2500 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 7867 |
| UniProt ID | Q16644 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway, VEGF signaling pathway |
| Gene Summary | This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200358 | MAPKAPK3 (Myc-DDK-tagged)-Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) |
CNY 3,656.00 |
|
| RC200358L1 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200358L2 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), mGFP tagged |
CNY 5,890.00 |
|
| RC200358L3 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200358L4 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), mGFP tagged |
CNY 5,890.00 |
|
| RG200358 | MAPKAPK3 (tGFP-tagged) - Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) |
CNY 5,256.00 |
|
| SC117238 | MAPKAPK3 (untagged)-Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) |
CNY 3,656.00 |
其它MAPKAP Kinase 3产品
