United States
Germany
Japan
United Kingdom
China
FGR (NM_005248) Human Untagged Clone
CAT＃: SC323444
FGR (untagged)-Kinase deficient mutant (K291M) of Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1
CNY 3,944.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-fgr; c-src2; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; SRC2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323444 sequence for NM_005248 edited (data generated by NextGen Sequencing)
ATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGC CTGGAAGGGGACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGCCTGACCCCACTAAG GCCCGGCCTGCATCCTCATTTGCCCACATCCCCAACTACAGCAACTTCTCCTCTCAGGCC ATCAACCCTGGCTTCCTTGATAGTGGCACCATCAGGGGTGTGTCAGGGATTGGGGTGACC CTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGGATGACCTCACCTTCACCAAG GGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGGAGGCTCGGTCTCTC AGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTGTTGACTCAATCCAA GCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGAGGCAGCTGCTTTCA CCAGGCAACCCCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCACCAAAGGTGCCTAC TCCCTGTCCATCCGGGACTGGGATCAGACCAGAGGCGATCATGTGAAGCATTACAAGATC CGCAAACTGGACATGGGCGGCTACTACATCACCACACGGGTTCAGTTCAACTCGGTGCAG GAGCTGGTGCAGCACTACATGGAGGTGAATGACGGGCTGTGCAACCTGCTCATCGCGCCC TGCACCATCATGAAGCCGCAGACGCTGGGCCTGGCCAAGGACGCCTGGGAGATCAGCCGC AGCTCCATCACGCTGGAGCGCCGGCTGGGCACCGGCTGCTTCGGGGATGTGTGGCTGGGC ACGTGGAACGGCAGCACTAAGGTGGCGGTGATGACGCTGAAGCCGGGCACCATGTCCCCG AAGGCCTTCCTGGAGGAGGCGCAGGTCATGAAGCTGCTGCGGCACGACAAGCTGGTGCAG CTGTACGCCGTGGTGTCGGAGGAGCCCATCTACATCGTGACCGAGTTCATGTGTCACGGC AGCTTGCTGGATTTTCTCAAGAACCCAGAGGGCCAGGATTTGAGGCTGCCCCAATTGGTG GACATGGCAGCCCAGGTAGCTGAGGGCATGGCCTACATGGAACGCATGAACTACATTCAC CGCGACCTGAGGGCAGCCAACATCCTGGTTGGGGAGCGGCTGGCGTGCAAGATCGCAGAC TTTGGCTTGGCGCGTCTCATCAAGGACGATGAGTACAACCCCTGCCAAGGTTCCAAGTTC CCCATCAAGTGGACAGCCCCAGAAGCTGCCCTCTTTGGCAGATTCACCATCAAGTCAGAC GTGTGGTCCTTTGGGATCCTGCTCACTGAGCTCATCACCAAGGGCCGAATCCCCTACCCA GGCATGAATAAACGGGAAGTGTTGGAACAGGTGGAGCAGGGCTACCACATGCCGTGCCCT CCAGGCTGCCCAGCATCCCTGTACGAGGCCATGGAACAGACCTGGCGTCTGGACCCGGAG GAGAGGCCTACCTTCGAGTACCTGCAGTCCTTCCTGGAGGACTACTTCACCTCCGCTGAA CCACAGTACCAGCCCGGGGATCAGACATAG Clone variation with respect to NM_005248.2 872 a=>t >OriGene 5' read for mutant NM_005248 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGCACTCGCGGC TGCTGGAGCCCCGGCTGGCTCACCCCGGGGCCGGGCAGAATTGGGCTCCAGGTCTCTGACCCCTCCCAAG GATCATGCCGCAGCCCCACTGACCCAGGAGTAGGGGCCTAAGGGCAGGGAACCTGGAATGGGCTGTGTGT TCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGCCTGGAAGGGACTTCAGAAAGCTA CGGGGCAGCAGACCACTATGGGCCTGACCCCCACTAAGGCCCGGCCTGCATCCCTCATTTGCCCCACATC CCCAACTACAGCAACTTCTCCTCTCAGGCCATCAACCCTGCTTCCTTGATAGTGGCACCATCAGGGTGTG TCAGGATGGGGTGACCTGGTCATTGCCTGTGACTTTAAGCTCGAACTAAGAGACTACTTCCCCAAGGCAA AAGTTCCATCCTGACATCCTGAGGAACTGTGAGCTCGTTCTCACTCGAAAACTGCTCATTCCAGCACACT GCCCTGTTGACTATCCACCTAAAAGTGACTTGAAATTGGAAAGGACGAAAGCATCTTTCACGACCAGGGC CTTACTCGGACAACCAGGCTTCGGTCTGTGTGTAAAAAA >SC323444 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CKGCRCGCGGCTGGGCACGGCTGCTTCGGGGATGTGTGGCTGGGCACGTGGAACGGCAGCACTAAGGTGG CGGTGATGACGCTGAAGCCGGGCACCATGTCCCCGAAGGCCTTCCTGGAGGAGGCGCAGGTCATGAAGCT GCTGCGGCACGACAAGCTGGTGCAGCTGTACGCCGTGGTGTCGGAGGAGCCCATCTACATCGTGACCGAG TTCATGTGTCACGGCAGCTTGCTGGATTTTCTCAAGAACCCAGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_005248 |
| Insert Size | 2450 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005248.1, NP_005239.1 |
| RefSeq Size | 2354 bp |
| RefSeq ORF | 1590 bp |
| Locus ID | 2268 |
| UniProt ID | P09769 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway |
| Gene Summary | This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) initiates at exon M4 and represents the longest transcript. It is expressed in myelomonocytic cells. Variants 1, 2, and 3 encode the same isoform. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214458 | FGR (Myc-DDK-tagged)-Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1 |
CNY 3,936.00 |
|
| RC214458L1 | Lenti ORF clone of Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1, Myc-DDK-tagged |
CNY 6,336.00 |
|
| RC214458L2 | Lenti ORF clone of Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC214458L3 | Lenti ORF clone of Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214458L4 | Lenti ORF clone of Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG214458 | FGR (tGFP-tagged) - Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1 |
CNY 5,536.00 |
|
| SC116870 | FGR (untagged)-Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1 |
CNY 3,944.00 |
