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CLK1 (NM_004071) Human Untagged Clone
CAT＃: SC323440
CLK1 (untagged)-Kinase deficient mutant (K191M) of Human CDC-like kinase 1 (CLK1)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLK; CLK/STY; STY |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323440 sequence for NM_004071 edited (data generated by NextGen Sequencing)
ATGAGACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGA AAATGGAGGAGCAGCAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAG AACAAGCGCTGCAAATACAATCACTCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGG TCTATAAATGAGAAAGATTATCATAGTCGACGCTACATTGATGAGTACAGAAATGACTAC ACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATGAAAGCCGGTATCAGAACCAT AGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACACAGGATTCAC CACAGTACTTCACATCGTCGTTCACATGGGNAGAGTCACCGAAGGAAAAGAACCAGGAGT GTAGAGGATGATGAGGAGGGTCACCTGATCTGTCAGAGTGGAGACGTACTAAGTGCAAGA NATGAAATTGTTGATACTTTAGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGAT CATAAAGCGGGAGGTAGACATGTAGCAGTAATGATAGTTAAAAATGTGGATAGATACTGT GAAGCTGCTCGCTCAGAAATACAAGTTCTGGAACATCTGAATACAACAGACCCCAACAGT ACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATCATGGTCACATTTGCATTGTT TTTGAACTATTGGGACTTAGTACTTACGACTTCATTAAAGAAAATGGGTTTCTACCATTT CGACTGGATCATATCAGAAAGATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCAC AGTAATAAGTTGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGAC TACACAGAGGCGTATAATCCCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGAT ATTAAAGTTGTAGACTTTGGTAGTGCAACATATGATGACGAACATCACAGTACATTGGTA TCTACAAGACATTATAGAGCACCTGAAGTTATTTTAGCCCTAGGGTGGTCCCAACCATGT GATGTCTGGAGCATAGGATGCATTCTTATTGAATACTATCTTGGGTTTACCGTATTTCCA ACACACGATAGTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTCTACCAAAA CATATGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATTAGACTGGGAT GAACACAGTTCTGCCGGCAGATATGTTTCAAGACGCTGTAAACCTCTGAAGGAATTTATG CTTTCTCAAGATGTTGAACATGAGCGTCTCTTTGACCTCATTCAGAAAATGTTGGAGTAT GATCCAGCCAAAAGAATTACTCTCAGAGAAGCCTTAAAGCATCCTTTCTTTGACCTTCTG AAGAAAAGTATATAG Clone variation with respect to NM_004071.3 391 a=>n;481 t=>n;572 a=>t;573 a=>g;768 t=>g >OriGene 5' read for mutant NM_004071 unedited
CGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACC GTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACGCAGTCAGCTG CGTGATTCCCGTGATTGCGTTACAAGCTTTGTCTCCTTCGACTTGGAGTCTTTGTCCAGGACGATGAGAC ACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGAGCAGCAG CAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCGCCAGGAGAACAAGCGCTGCAAATACAATCACTC TAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAAGATTATCATAGTCGACGCTA CATTGATGGAGTACAGAAATGACTCACTCAGGGATGGTGACCCTGGGACATCGCCAAGAAGACAATGAAA CCCGTTATCAAACCCATGGTAGCAGTTCTTTCGGGTAAAGTGGGAGAAGGTGGTTATAAAGCCAAAACCA GGATTACCCAGGACCTTACCACCGGTGTTTCATTGGGGAAAGACCGTTTTAAAACATTTGGAATTTATTC CATGGGAACGGGGAACTTGATTTGTGTGATATATTAAAAGTTTTAAAAGATCTCTCACTTTTACCGGGAG ATTTTTGTGTGTTGCCTGGGAGGAATTCCAGAAAAGACACATTGTGTCAATATATATGTGTGGCTCATGC ACCCGGAGATGAGGGTATAGTGTATTTGAAGTTGAAATTTCCAAGTCTCACGGTTGTGTGCTCCGAACTG CTATGTTCTAGTACCCAGTGACCGACGCTCTGGTACTCTAAGATGTCGTCTGCGCATAGTATATC >SC323440 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGAMGTMTAGTGCAGATGTWAGAATATTTTTCACACTTATTAACTTTTCAGATAACATAATCTATATATA GATTAAGCTTTCAGGGATTTGGAAATCTTTTTTTCTTTCTCTTTTTTGTTTTTGTTTTATTTTTCCATTT CTTTTGGTGGGGGGGATTGTATTTTTGCTTTCTTTAGAAATGTAATGTTTGTTATATAGAACTTCCAGAA CAGTAATCAAATTAATGAAATTAGACCTAATAATTATGTTTTTTG |
| Restriction Sites | Please inquire |
| ACCN | NM_004071 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004071.2, NP_004062.2 |
| RefSeq Size | 1887 bp |
| RefSeq ORF | 1455 bp |
| Locus ID | 1195 |
| UniProt ID | P49759 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009] Transcript Variant: This variant (1) encodes the shorter isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204443 | CLK1 (Myc-DDK-tagged)-Human CDC-like kinase 1 (CLK1), transcript variant 1 |
CNY 3,656.00 |
|
| RC204443L1 | Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204443L2 | Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204443L3 | Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204443L4 | Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG204443 | CLK1 (tGFP-tagged) - Human CDC-like kinase 1 (CLK1), transcript variant 1 |
CNY 5,256.00 |
|
| SC117574 | CLK1 (untagged)-Human CDC-like kinase 1 (CLK1), transcript variant 1 |
CNY 3,656.00 |
|
| SC323558 | CLK1 (untagged)-Kinase deficient mutant (K191M) of Human CDC-like kinase 1 (CLK1) |
CNY 3,656.00 |
|
| SC324172 | CLK1 (untagged)-Human CDC-like kinase 1 (CLK1), transcript variant 1 |
CNY 3,656.00 |
