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FYN (NM_002037) Human Untagged Clone
CAT＃: SC323428
FYN (untagged)-Kinase deficient mutant (K299M) of Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1
CNY 6,008.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | p59-FYN; SLK; SYN |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002037, the custom clone sequence may differ by one or more nucleotides
ATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAACAAAACTGACGGAGGAGAGGGACGGCAGCCTGAACC AGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCCAGCTTCGGTGTGACCTCCAT CCCCAACTACAACAACTTCCACGCAGCCGGGGGCCAAGGACTCACCGTCTTTGGAGGTGTGAACTCTTCG TCTCATACGGGGACCTTGCGTACGAGAGGAGGAACAGGAGTGACACTCTTTGTGGCCCTTTATGACTATG AAGCACGGACAGAAGATGACCTGAGTTTTCACAAAGGAGAAAAATTTCAAATATTGAACAGCTCGGAAGG AGATTGGTGGGAAGCCCGCTCCTTGACAACTGGAGAGACAGGTTACATTCCCAGCAATTATGTGGCTCCA GTTGACTCTATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGAAAAGATGCTGAGCGACAGCTAT TGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGTGAAACCACCAAAGGTGCCTATTCACT TTCTATCCGTGATTGGGATGATATGAAAGGAGACCATGTCAAACATTATAAAATTCGCAAACTTGACAAT GGTGGATACTACATTACCACCCGGGCCCAGTTTGAAACACTTCAGCAGCTTGTACAACATTACTCAGAGA GAGCTGCAGGTCTCTGCTGCCGCCTAGTAGTTCCCTGTCACAAAGGGATGCCAAGGCTTACCGATCTGTC TGTCAAAACCAAAGATGTCTGGGAAATCCCTCGAGAATCCCTGCAGTTGATCAAGAGACTGGGAAATGGG CAGTTTGGGGAAGTATGGATGGGTACCTGGAATGGAAACACAAAAGTAGCCATAAAGACTCTTAAACCAG GCACAATGTCCCCCGAATCATTCCTTGAGGAAGCGCAGATCATGAAGAAGCTGAAGCACGACAAGCTGGT CCAGCTCTATGCAGTGGTGTCTGAGGAGCCCATCTACATCGTCACCGAGTATATGAACAAAGGAAGTTTA CTGGATTTCTTAAAAGATGGAGAAGGAAGAGCTCTGAAATTACCAAATCTTGTGGACATGGCAGCACAGG TGGCTGCAGGAATGGCTTACATCGAGCGCATGAATTATATCCATAGAGATCTGCGATCAGCAAACATTCT AGTGGGGAATGGACTCATATGCAAGATTGCTGACTTCGGATTGGCCCGATTGATAGAAGACAATGAGTAC ACAGCAAGACAAGGTGCAAAGTTCCCCATCAAGTGGACGGCCCCCGAGGCAGCCCTGTACGGGAGGTTCA CAATCAAGTCTGACGTGTGGTCTTTTGGAATCTTACTCACAGAGCTGGTCACCAAAGGAAGAGTGCCATA CCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAGCGAGGCTACAGGATGCCCTGCCCGCAGGAC TGCCCCATCTCTCTGCATGAGCTCATGATCCACTGCTGGAAAAAGGACCCTGAAGAACGCCCCACTTTTG AGTACTTGCAGAGCTTCCTGGAAGACTACTTTACCGCGACAGAGCCCCAGTACCAACCTGGTGAAAACCT GTAA >OriGene 5' read for mutant NM_002037 unedited
ACCGCCCGTCCAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTTTTTGAA GAAGCAGGATGCTGACTCTAAACGTGGAAAAAGACCAGTCCTGCCTCTGTTGTAGAAGACATGTGGTGTA TATAAAGCTTGTGATCGTTGGCGGAAATTTTGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAA GAAGCAACAAAACTGACGGAGGAGAGGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAG ACCCCACCCCTCAGCACTACCCCCAGCCTTCGGGTGGTGACCTCCATCCCCAACTACAACCAACTTTCCA CGCCAGCCCGGGGGCAAAGGACTCCCCGTCTTTGGAGGGTGTGAACCTCTTCTTCTATTACGGGAACCTT GCGTACAAAAGGAGGAACAGAGATTGACACTCTTGGTGGCCCTTTATAACAATGAGGAACGGACGGAGAA TGACTGGAGTTTTCAAAAAGGAAAAAAATTCAAATATTTAAAAGCCCTGGAAAGAGATTGGGGGGGAACC CCGCTCTCTGACCAATGGGGAGACACGTTACATTTCCAGCGCATATTGTGGGGCCCCGTTAGACTCTACC CACGCAGAGAGGTGACACTTTGAAACCTCTGCCAAAGATGCTGAACACAACTCTTGTGCTCTTTGAACCC AGAGAGCCTTCTTATACGCGAGATGAGAACACCCAAGTGGCCATACACTTCTACCTGATTGGATGATGAA AGGAACACTGTCACACTATAAATCCCACTTGACATGTGTATCACGTAACCGCAGTGACCTCACGACTTGC CAATCCAAGGTCAGTCTGCGCGAATTCGTCAGATGCAGCTA >SC323428 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GTGWCAGAGMTGGGAATGGGCAGTTTGGGGAAGTATGGATGGGTACCTGGAATGGAAACACAAAAGTAGC CATAATGACTCTTAAACCAGGCACAATGTCCCCCGAATCATTCCTTGAGGAAGCGCAGATCATGAAGAAG CTGAAGCACGACAAGCTGGTCCAGCTCTATGCAGTGGTGTCTGAGGAGCCCATCTACATCGTCACCGAGT ATATGAACAAAGGAAGTTTACTGGATTTCTTAAAAGATGGAGAAG |
| Restriction Sites | Please inquire |
| ACCN | NM_002037 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002037.3, NP_002028.1 |
| RefSeq Size | 2650 bp |
| RefSeq ORF | 1614 bp |
| Locus ID | 2534 |
| UniProt ID | P06241 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis |
| Gene Summary | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224691 | FYN (Myc-DDK-tagged)-Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1 |
CNY 6,000.00 |
|
| RC224691L1 | Lenti ORF clone of Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, Myc-DDK-tagged |
CNY 8,400.00 |
|
| RC224691L2 | Lenti ORF clone of Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, mGFP tagged |
CNY 8,400.00 |
|
| RC224691L3 | Lenti ORF clone of Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC224691L4 | Lenti ORF clone of Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, mGFP tagged |
CNY 8,400.00 |
|
| RG224691 | FYN (tGFP-tagged) - Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1 |
CNY 7,600.00 |
|
| SC118862 | FYN (untagged)-Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1 |
CNY 6,008.00 |
